Cordyceps militaris illumination culture method

A technology for light cultivation and Cordyceps militaris, applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems of fruit body bending, affecting the harvesting, processing and selling of Cordyceps militaris, and achieve the effect of improving the survival rate.

Inactive Publication Date: 2018-01-09
宁波佗鹊堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Cordyceps militaris has phototaxis, which causes the fruiting body to bend and grow, which affects the harvesting, processing and selling of the fruiting body of Cordyceps militaris

Method used

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  • Cordyceps militaris illumination culture method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Cordyceps militaris light culture method, comprises the following steps:

[0023] Step 1: Prepare medium: Stir 50 parts of cicada pupae powder, 50 parts of walnut shell powder, 5 parts of glucose, 2 parts of magnesium sulfate, 0.2 parts of vitamin B1 and 15 parts of water into a paste by weight, and then make a 5cm ×5cm×5cm brick-shaped culture medium;

[0024] Step 2: Sterilization: Put the culture medium at 110°C to sterilize for more than 30 minutes, then cool it down to room temperature naturally, clean and disinfect the incubator with 8% potassium permanganate solution, and then dry it;

[0025] Step 3: Embedding: embedding the inoculated pupal body in the culture medium;

[0026] Step 4: Cultivation: Use a Cordyceps incubator to cultivate for 80-100 days, and the Cordyceps incubator is as attached figure 1 As shown, it includes a closed culture medium box 2 and a lampshade 3 that can isolate light. The lampshade 3 is fastened on the top of the culture medium box...

Embodiment 2

[0029] The only difference with Embodiment 1 is:

[0030] Step 1: Prepare medium: Stir 60 parts of cicada pupae powder, 60 parts of walnut shell powder, 10 parts of glucose, 3 parts of magnesium sulfate, 0.5 parts of vitamin B1 and 30 parts of water into a paste by weight, and then make a 5cm ×5cm×5cm brick-shaped culture medium;

[0031] Step 4: The illuminance of the LED lamp 7 is 240 lux. In the mycelial growth stage, add distilled water through the water pipe 5 to make the water content of the culture medium 65%, the temperature is 22°C, and the relative humidity is 70%. Turn off the LED lamp 7; After the fruit body reproductive growth stage, use sterilized tweezers to fiddle with the 3 fruit bodies in the early growth stage into each culture tube 6, turn on the LED light 7, add distilled water through the water supply pipe 5 to make the water content of the medium 70%, and the temperature 23 ℃, air relative humidity 90%.

[0032] Get the fruiting bodies harvested in emb...

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Abstract

The invention relates to the technical field of culture, and discloses a cordyceps militaris illumination culture method. The method comprises the following steps of preparing a culture medium, wherein cicada chrysalis powder, walnut shell flour, glucose, magnesium sulfate, vitamin B1 and water are stirred into a paste, and then a 5cm*5cm*5cm brick-shaped culture medium is obtained; performing sterilization, wherein the culture medium is sterilized; burying seeds, wherein inoculated pupa bodies are buried into the culture medium; performing culturing, wherein a cordyceps sinensis incubator isused for culturing for 80-100 days, culture conditions are controlled through the incubator, and according to the culture conditions, in the mycelial growth stage, water is added through a water adding pipe, the water content of the culture medium is 60-65%, the temperature is 18-22 DEG C, the relative humidity is 60-70%, and an LED lamp is turned off; after the sporocarp reproductive growth stageis reached, the LED lamp is turned on, the water content of the culture medium is 65-70%, the temperature is 20-23 DEG C, and the relative air humidity is 80-90%; harvesting the sporocarp, wherein the growing sporocarp is harvested through tweezers. According to the cordyceps militaris illumination culture method, the linear sporocarp cordyceps militaris is cultured.

Description

technical field [0001] The invention relates to the field of cultivation technology. Background technique [0002] Cordyceps militaris is a model species of Ascomycetes subphylum, Ergotaceae, Ergotaceae, Cordyceps; also known as Cordyceps militaris, Cordyceps militaris, Cordyceps militaris, etc.; It is cheap and contains 16 times more nutritious cordycepin than wild Cordyceps sinensis. It has been proven to have a positive therapeutic effect on lymphoma and some leukemias, and its application prospects are very broad. [0003] The artificial large-scale cultivation of Cordyceps militaris needs to plant the pupae inoculated with Cordyceps militaris in the medium, and the Cordyceps militaris germinates on the pupae to form mycelium, and the mycelium decomposes various tissues and organs in the pupae, grow into fruiting bodies. [0004] The traditional large-scale cultivation device of Cordyceps militaris is to cultivate the glass bottles or plastic boxes inoculated with Cord...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20C05G1/00
Inventor 王健
Owner 宁波佗鹊堂生物科技有限公司
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