Method for preparing flocculant by using compound bacterial agent

A technology of composite bacterial agent and flocculant, applied in the field of water purification, can solve the problems of increased sludge treatment load, color, strong carcinogenicity, etc.

Active Publication Date: 2020-07-07
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aluminum ions in aluminum-based flocculants can easily cause Alzheimer's disease; iron ions in iron-based flocculants have a corrosive effect on metals, which can easily cause color in the treated water and increase the load of sludge treatment; PAM monomers have strong neurotoxicity. Toxic, highly carcinogenic

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Pseudoalteromonas Pseudoalteromonas sp. GHS18 and Pseudomonas alternata Pseudoalteromonas A flocculant made from the exopolysaccharide obtained by co-fermentation of sp. JP. Among them, Pseudoalteromonas Pseudoalteromonas The taxonomic designation of sp. GHS18 is Pseudoalteromonas ( Pseudoalteromonas sp.), was deposited on August 29, 2016 in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microorganisms, with the preservation number CGMCC No. 12913. Pseudomonas alternata Pseudoalteromonas The taxonomic designation of sp. JP is Pseudomonas alternata ( Pseudoalteromonas sp.), was deposited on August 29, 2016 in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures, and the preservation number is CGMCC No. 12914. The General Microorganism Center (CGMCC) of China Committee for Culture Collection of Microorganisms is located at No. 3, Yard No. 1, Beichen West Road, Chaoyang District...

Embodiment 2

[0031] The method for preparing a flocculant by using a composite bacterial agent comprises the following steps:

[0032] 1) Seed liquid culture: the seed liquid culture step is to put Pseudoalteromonas Pseudoalteromonas sp. GHS18 was inoculated on the slant medium, and cultured at 25°C for 24 hours, then added sterile water 1.3 times the volume of the medium, and oscillated to obtain seed solution 1; the strain was replaced by Pseudomonas alternata Pseudoalteromonas sp. JP, repeat the above steps to obtain seed solution 2, mix seed solution 1 and seed solution 2 at a mass ratio of 1:2 to obtain seed solution 3. The composition of the slant medium is: glucose 22g / L, urea 0.48g / L, ammonium sulfate 0.20g / L, yeast extract 0.6g / L, dipotassium hydrogen phosphate 3.1 g / L, potassium dihydrogen phosphate 1.2g / L, agar 15g / L and the rest artificial seawater; adjust the pH to 7.3, autoclave at 115°C for 30min. Among them, the artificial seawater formula is: sodium chloride 25.0 g / L, ma...

Embodiment 3

[0036] Example 3: Determination of flocculation activity

[0037] Adopt flocculation activity test method (R.Kurane, K.Takeda, T.Suzuki.Screening and Characteristics of Microbial Flocculants.Agric.Biol.Chem.1986: 2301-2307) to measure the flocculation activity of kaolin suspension, take 5g / L of kaolin suspension 93ml, add 5ml 1% (m / v) CaCl 2 As a coagulation aid, add 2ml of the sample of Example 1, adjust the pH value to 9, mix and stir quickly for 1min, then slowly stir for 5min, let stand for 10min, take the mixed solution at 1cm below the water level, and measure the absorbance A at 550nm with a visible spectrophotometer , while using 2ml of distilled water instead of the sample as a control, measure the absorbance B, and calculate the flocculation rate, FR(%) = (A-B) / A×100%. Then the flocculation rate of the flocculant to the kaolin suspension is 93.17%.

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PUM

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Abstract

The invention discloses a method for preparing a flocculating agent by use of a compound microbial agent. A remarkable synergetic function is produced on growth and reproduction of Pseudoalteromonas sp. GHS18 and Pseudoalteromonas sp. JP, and the prepared flocculating agent has the decolorization flocculation effect superior to that obtained through fermentation by single strains. The preparationmethod of the flocculating agent comprises steps as follows: culture of a seed solution, preparation of a liquid culture medium, expanded fermenting culture and preparation of the flocculating agent.A hydrolysate obtained by hydrolyzing crop waste with inorganic acid is used for replacing a carbon source in the culture medium, and the cost is saved; sandy soil is taken as a carrier, so that the strains are attached to the carrier, and effective ingredients of the flocculating agent can be simply and thoroughly separated from a culture solution.

Description

technical field [0001] The invention relates to the technical field of water purification, in particular to a method for preparing a flocculant by using a composite microbial agent. Background technique [0002] Flocculation technology is widely used in water supply, wastewater flocculation purification, fermentation industry, solid-liquid separation, etc. The flocculation effect depends on the type of flocculant selected. At present, there are two main types of flocculants used in the water treatment industry: inorganic flocculants represented by aluminum-based, iron-based and their polymers, and organic polymer flocculants represented by PAM and their derivatives. They are widely used due to their good flocculation effect and low cost during use, but their unsafety to the environment has attracted enough attention. Aluminum ions in aluminum-based flocculants can easily cause Alzheimer's disease; iron ions in iron-based flocculants have a corrosive effect on metals, which ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12N1/20C12R1/01
Inventor 穆军杨桥崔霞王东方王玉霞
Owner ZHEJIANG OCEAN UNIV
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