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Application of VDAC1 protein to preparation of breast cancer postoperative prognosis evaluation kit, breast cancer postoperative prognosis evaluation kit and method

A technology for prognostic assessment and breast cancer, applied in the biological field, can solve problems such as unclear molecular mechanism and lack of specific molecular targets for breast cancer treatment

Inactive Publication Date: 2018-01-12
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Breast cancer is one of the most threatening tumors to women's health. So far, the molecular mechanism of its occurrence is still unclear, and the treatment of breast cancer also lacks specific molecular targets. VDAC1, as a very important tumor oncogene, is currently There is no literature report that VDAC1 protein is related to breast cancer prognosis or breast cancer metastasis

Method used

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  • Application of VDAC1 protein to preparation of breast cancer postoperative prognosis evaluation kit, breast cancer postoperative prognosis evaluation kit and method
  • Application of VDAC1 protein to preparation of breast cancer postoperative prognosis evaluation kit, breast cancer postoperative prognosis evaluation kit and method
  • Application of VDAC1 protein to preparation of breast cancer postoperative prognosis evaluation kit, breast cancer postoperative prognosis evaluation kit and method

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Experimental program
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Embodiment 1

[0043] A kit for postoperative prognosis assessment of breast cancer, the kit includes: human VDAC1 monoclonal antibody or VDAC1 polyclonal antibody for detecting the relative expression of VDAC1 protein in breast cancer tissue, immunohistochemical experiment reagent. Among them, immunohistochemical reagents include: xylene, ethanol, 3% H 2 o 2 , 3% BSA blocking solution, DAB chromogenic reagent, hematoxylin, horseradish peroxidase, PBS and 0.01M EDTA repair solution.

[0044] The human VDAC1 polyclonal antibody of the present invention is obtained by immunizing rabbits with the VDAC1 protein whose sequence is shown in SEQ ID NO.1. It can be prepared by itself, or commercially available products can be used.

[0045] Immunohistochemical experiment reagent of the present invention comprises: Xylene, ethanol, 3%H 2 o 2 (aqueous solution), 3% BSA blocking solution (prepared in PBS), DAB chromogenic reagent, hematoxylin, horseradish peroxidase (for labeling secondary antibody...

Embodiment 2

[0047] The application of VDAC1 protein in the preparation of breast cancer postoperative prognosis assessment kit, this application is to use the kit of the present invention to carry out breast cancer postoperative prognosis assessment, the method is as follows:

[0048] (1) Take pathological specimens of breast cancer patients, and use human VDAC1 monoclonal antibody or human VDAC1 polyclonal antibody, as well as immunohistochemical experimental reagents, for immunohistochemical staining;

[0049] Among them, the pathological specimens come from the biopsy tissue of breast cancer patients or the pathological materials taken during and after the operation. The immunohistochemical method uses the SP staining method, and the specific steps are as follows:

[0050] (a) Paraffin sections of breast cancer tissues were prepared, and the oven was set at 60° C. overnight.

[0051] (b) Dewaxing of slices. Soak in sequence: Xylene I: 10min; Xylene II: 10min; Xylene III: 10min.

[0...

Embodiment 3

[0070] A breast cancer postoperative tumor sample was taken for paraffin-embedded section, and detected by the above-mentioned immunohistochemical method. After calculation, the VDAC1 immunohistochemical score of the cancer tissue was 0.695. After postoperative follow-up, it was found that the patient had recurrence and metastasis of breast cancer in the 10th month after the operation, and died 16 months after the operation.

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Abstract

The invention provides application of a VDAC1 protein to preparation of a breast cancer postoperative prognosis evaluation kit, the breast cancer postoperative prognosis evaluation kit and a method. The relative expression quantity of the VDAC1 protein in the breast cancer tissue is detected by an immunohistochemical method, so that the risk that breast cancer recurrence and metastasis occurs in patients suffering from breast cancer is judged. The invention has the following beneficial effects: the invention provides application of the VDAC1 protein to preparation of the breast cancer postoperative prognosis evaluation kit, and indicates that the protein can be used for preparing a protein molecular maker for judging prognosis of the patients suffering from the breast cancer and has important guiding significance in prognosis monitoring and sequential treatment of the patients suffering from the breast cancer.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of VDAC1 protein in the preparation of a breast cancer postoperative prognosis evaluation kit, a breast cancer prognosis evaluation kit and a method. Background technique [0002] The female mammary gland is composed of skin, fibrous tissue, mammary glands and fat. Breast cancer is a malignant tumor that occurs in the mammary gland epithelial tissue. 99% of breast cancers occur in women and only 1% in men. [0003] The mammary gland is not an important organ to maintain human life activities, and breast cancer in situ is not fatal; but because breast cancer cells lose the characteristics of normal cells, the connections between cells are loose and easy to fall off. Once the cancer cells fall off, the free cancer cells can spread throughout the body with the blood or lymph fluid, forming metastasis, which is life-threatening. At present, breast cancer has...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68
Inventor 林尧王清水
Owner FUJIAN NORMAL UNIV