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A hyaluronic acid-mediated method for synthesizing and modifying cerium nanometer quantum dots and its products and applications

A technology of nano-quantum dots and hyaluronic acid, which is applied in the field of nano-materials, can solve the problems that have not been seen and have not been seen based on hyaluronic acid-mediated synthesis and modification of cerium nano-quantum dots, and achieve stability and biocompatibility guarantee, Significant impetus, application prospects, flexible and easy-to-control effects of regulation methods

Active Publication Date: 2019-03-26
重庆市人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has not been any literature report on the modification of single cerium nanomaterials by HA molecule-mediated synthesis, let alone any literature report on the modification of cerium nano-quantum dots based on hyaluronic acid-mediated synthesis.

Method used

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  • A hyaluronic acid-mediated method for synthesizing and modifying cerium nanometer quantum dots and its products and applications
  • A hyaluronic acid-mediated method for synthesizing and modifying cerium nanometer quantum dots and its products and applications
  • A hyaluronic acid-mediated method for synthesizing and modifying cerium nanometer quantum dots and its products and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, the preparation of cerium nano quantum dots (HA molecular weight: 17kd; Cerium salt: Ce(NO 3 ) 3 .6H 2 O)

[0032] Take 50mg HA (molecular weight 17kd) and add it into 8.7mL distilled aqueous solution, take 500μL 0.1mol / L Ce(NO 3 ) 3 ·6H2 O was added to it, and under 600r / min magnetic stirring, after reacting at room temperature (25°C) for 30 minutes, 800 μL of 1mol / L NaOH was added to the above system, and the reaction was stopped after continuing the magnetic stirring reaction for 6 hours. Distilled water was dialyzed into a dialysis bag with a molecular weight cut off of 10,000 for 24 hours, and the water was changed every 6 hours, and the water change volume was 3 L / time. To remove unreacted cerium salt, hydroxide (OH - ) and other inorganic ions. Finally, it was placed in a 100kd (15mL) sleeve centrifuge tube and washed by centrifugation at 4500r / min for 4 times to remove excess unreacted HA to obtain cerium nano-quantum dots (HA@CQDs-1, 5mL, wi...

Embodiment 2

[0033] Embodiment 2, the preparation of cerium nano quantum dots (HA molecular weight: 5kd; Cerium salt: Ce(NO 3 ) 3 .6H 2 O)

[0034] Take 50mg HA (molecular weight 5kd) and add it into 8.5mL distilled aqueous solution, take 500μL 0.1mol / L Ce(NO 3 ) 3 ·6H 2 Add O to it, under 600r / min magnetic stirring, react at room temperature (25°C) for 15 minutes, take 1000 μL of 1mol / L NaOH and add it to the above system, continue the magnetic stirring reaction for 24 hours, stop the reaction, and place the above reaction solution in the Distilled water into a dialysis bag with a molecular weight cut off of 10,000 for dialysis for 24 hours, wherein the water was changed every 6 hours, and the water change volume was 3 L / time. To remove unreacted cerium salt, hydroxide (OH - ) and other inorganic ions. Finally, it was placed in a 100kd (15mL) sleeve centrifuge tube and washed by centrifugation at 4500r / min for 4 times to remove excess unreacted HA to obtain cerium nano-quantum dots...

Embodiment 3

[0035] Embodiment 3, the preparation of cerium nano quantum dots (HA molecular weight: 45kd; Cerium salt: Ce(NO 3 ) 3 .6H 2 O)

[0036] Take 50mg HA (molecular weight 45kd) and add it into 8.5mL distilled aqueous solution, take 500μL 0.1mol / L Ce(NO 3 ) 3 ·6H 2 Add O to it, under 1000r / min magnetic stirring, react at room temperature (25°C) for 1h, take 1000μL of 1mol / L NaOH and add it to the above system, continue the magnetic stirring reaction for 24h, stop the reaction, and place the above reaction solution in the Distilled water was dialyzed into a dialysis bag with a molecular weight cut off of 10,000 for 24 hours, and the water was changed every 6 hours, and the water change volume was 3 L / time. To remove unreacted cerium salt, hydroxide (OH - ) and other inorganic ions. Finally, it was placed in a 100kd (15mL) sleeve centrifuge tube and washed by centrifugation at 4500r / min for 4 times to remove excess unreacted HA to obtain cerium nano-quantum dots with an averag...

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Abstract

The invention relates to a method for synthesizing modified cerium nanometer quantum dots under mediation of hyaluronic acid and a product and application thereof. The method comprises the following specific steps: dissolving hyaluronic acid by adding water, then adding trivalent cerium salt, reacting for 0.5-1h under a magnetic stirring condition to synthesize a precursor, then adding an alkalinesolution into a reaction system, performing a nucleating and curing reaction, and purifying to remove unreacted inorganic ions and the unreacted hyaluronic acid to obtain the hyaluronic acid-coated cerium nanometer quantum dots. By the method, the reaction is environmentally friendly and economical, the regulative way is flexible and easy to control, and the repeatability is strong; the obtainedquantum dots are uniform in shape and size and good in dispersibility and stability; the HA for modifying the surfaces of the cerium nanometer quantum dots has excellent biocompatibility and a large amount of carboxyl functional groups for modifying, so that the HA can further modify other functional molecules in a grafting manner, and a foundation is laid for further in-vivo application of the cerium nanometer quantum dots.

Description

technical field [0001] The invention belongs to the field of nanomaterials, and relates to a hyaluronic acid-mediated method for synthesizing and modifying cerium nanometer quantum dots, as well as products and applications prepared by the method. Background technique [0002] Ce nanomaterials due to surface Ce 3+ / C 4+ Coexistence and mutual conversion, so that it has a variety of simulated enzymatic activities, such as simulated superoxide dismutase, catalase, phosphatase, oxidase, etc. (Nanoscale.2011, 3, 1411-1420, NPG Asia Mater. 2014, 6, e90). Therefore, it can effectively scavenge a variety of active free radicals, such as O 2 ·, H 2 o 2 , OH·, NO·, ONOO·, etc., and at the same time have the high efficiency, long-term effect and reproducibility of free radical scavenging [Antioxidants,2016,5(2):15]. It has very important and broad application prospects in chemical catalytic reaction, radiation protection, tumor radiotherapy and chemotherapy, treatment of antioxi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/61A61K33/24B82Y40/00B82Y5/00A61P39/06A61P17/18A61P35/00B82Y30/00B22F1/00B22F9/24
Inventor 刘俊利曾一平郝玉徽曾伟南谭祖键周明全贾小林张胜利
Owner 重庆市人民医院
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