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TabHLH44 protein and coding gene and application thereof

A protein and coding technology, applied in the field of TabHLH44 protein and its encoding gene and application, can solve the problem of less research on the function of TabHLH transcription factor

Active Publication Date: 2018-01-19
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is relatively little research on the function of the TabHLH transcription factor related to stress resistance in wheat

Method used

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  • TabHLH44 protein and coding gene and application thereof
  • TabHLH44 protein and coding gene and application thereof
  • TabHLH44 protein and coding gene and application thereof

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Experimental program
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Embodiment 1

[0048] Embodiment 1, the discovery of TabHLH44 gene

[0049] 1. Discovery of the TabHLH44 gene

[0050] The previous work of the laboratory constructed several full-length cDNA libraries of wheat. By sequencing the cDNA plasmids, the bHLH transcription factor was predicted according to the structural characteristics of the transcription factor, and the full-length cDNA sequence of the TabHLH44 gene was obtained.

[0051] The nucleotide sequence of the TabHLH44 gene in wheat is sequence 1, and the open reading frame is sequence 1 nucleotides 271-1458; the amino acid sequence of the encoded protein TabHLH44 is sequence 2.

[0052] 2. Expression of TabHLH44 gene under stress conditions

[0053] Chinese spring, drought-resistant wheat Hanxuan No. 10, and salt-resistant wheat Chadianhong used for stress treatment were cultured at 25°C for 10 days under a 16h light / 8h dark photoperiod, and then subjected to different stress treatments. Chinese spring was used for cold treatment at...

Embodiment 2

[0055] Embodiment 2, the application of TabHLH44 gene in stress resistance

[0056] 1. Preparation of transgenic Arabidopsis thaliana

[0057] 1. Using the Gateway method to construct an overexpression vector for Arabidopsis transformation

[0058] The BP plasmid is to insert the TabHLH44 gene shown in the 271-1458th position of sequence 1 into the entry vector pDONR TM / Zeo (Invitrogen, 12535035) obtained plasmid.

[0059] LR reaction: The above-mentioned BP plasmid and pEarleyGate 100 overexpression vector were subjected to LR reaction by the method of Gateway technology to obtain a recombinant vector. The LR reaction system is shown in Table 2:

[0060] Table 2 is the LR response

[0061]

[0062] The recombinant vector is a vector obtained by inserting nucleotides 271-1458 of sequence 1 between the two attB sites of the pEarleyGate 100 vector, and is a vector for overexpressing the TabHLH44 gene, named pEarleyGate 100-TabHLH44.

[0063] 2. Transformation of Agrobac...

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Abstract

The invention discloses TabHLH44 protein and a coding gene and application thereof. The invention provides the protein called TabHLH44 which is described as (1) or (2): (1) protein represented by a sequence 2 in a sequence list, and (2) protein which has the same functions as the protein represented by the sequence 2 and is derived from the sequence 2 by subjecting the protein sequence representedby the sequence 2 in the sequence list to substitution and / or deficiency and / or addition of one or more amino acid residues. According to experiments of the invention, it is proven that the transcription factor TabHLH44 gene which responds to adversity stress is screened by conducting treatment of adversity stress on wheat, and is induced by salt, PEG, cold stress and ABA, and by introducing thetranscription factor TabHLH44 gene into Arabidopsis thaliana, the arabidopsis thaliana modified by the TabHLH44 gene is obtained; by identifying the functions of the arabidopsis thaliana modified by the TabHLH44 gene, it is indicated that the drought resistance, salt resistance and frost resistance of the transgenic arabidopsis thaliana of the transgenic arabidopsis thaliana are enhanced through the TabHLH44 gene, and a basis is provided for breeding of resistant plants.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a TabHLH44 protein and its coding gene and application. Background technique [0002] Harsh growth environment (extreme temperature, salinity, drought, etc.) seriously affects the growth and development of plants and reduces the yield of crops. Plants cannot avoid adversity stress by moving, so in order to eliminate or reduce the harm caused by adversity stress, plants have formed a complex regulatory network in the process of evolution, so that plants can pass cellular level, molecular Levels and other adjustments to adapt to unsuitable growth environments. In order to reduce the huge loss of crops caused by unfavorable growth environment and ensure food security, how to improve the stress resistance of crops has attracted the attention of governments all over the world. It is an effective method to genetically improve crops to improve their stress resistance. [0003] Transcript...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/11A01H5/00A01H6/20
Inventor 孔秀英翟亦倩张立超夏川符思路赵光耀贾继增
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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