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Use of estrogen-related receptor alpha as diagnostic marker for glioma and related application

A diagnostic marker, estrogen technology, applied in the field of molecular biology and tumor prevention and treatment, can solve problems such as pain and burden, and achieve the effect of reducing recurrence and improving survival rate

Inactive Publication Date: 2018-01-19
复旦大学附属华山医院北院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, glioma has caused a huge burden and pain to the patient's family and society, and has become one of the malignant tumors that seriously affect human health, and the factors that affect the prognosis of patients with glioma are also very complicated.

Method used

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  • Use of estrogen-related receptor alpha as diagnostic marker for glioma and related application
  • Use of estrogen-related receptor alpha as diagnostic marker for glioma and related application
  • Use of estrogen-related receptor alpha as diagnostic marker for glioma and related application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The effects of XCT790, ERRα shRNA and lentivirus on the transcriptional activity of ERRα gene in tissues and cells were tested by fluorescent quantitative PCR experiment.

[0031] This example illustrates that XCT790 and ERRα shRNA involved in the present invention can effectively inhibit the transcription of ERRα gene in glioma cell lines U87MG and U251, indicating that 1) ERRα mRNA is expressed in glioma cell lines U87MG and U251; 2) using XCT790 or ERRα shRNA significantly down-regulated ERRα mRNA levels in glioma cell lines U87MG and U251; 3) Lentivirus-mediated overexpression of ERRα significantly up-regulated ERRα mRNA levels in glioma cell lines U87MG and U251. Fluorescence quantitative PCR testing technology is a technology familiar to those skilled in the art.

[0032] 1. Cell plating:

[0033] Warm 10% FBS DMEM cell culture solution and trypsin at 37°C in advance, wipe and sterilize the aseptic operating table with alcohol, then put the trypsin and DMEM cell ...

Embodiment 2

[0064] Western Bloting was used to test the expression of ERRα in human normal brain tissue and glioma and the effect of ERRα shRNA on the expression level of ERRα protein in glioma cell lines. Western Blotting experiments are techniques familiar to those skilled in the art.

[0065] 1. Extract tissue and cell total protein

[0066] After the cells were intervened for a corresponding period of time, the old culture medium in the original cell culture class was discarded, and the cells were washed 2-3 times with pre-cooled PBS, and 200 μL of cell RIPA lysate was added to each well of a 6-well plate (the Add PMSF 10 minutes before the final concentration of 1 μM), and blow it with a gun for a few times to make the lysate fully contact with the cells. After 1-2s, the cells are fully lysed. After sufficient lysis, cells were harvested on ice with a cell scraper. Use a pipette gun to transfer the lysate into a 1.5mL centrifuge tube, blow and mix well, and place on ice for 30min....

Embodiment 3

[0096] CCK-8 cell proliferation assay was used to determine the effect of XCT790, ERRα shRNA and lentivirus on the proliferation of glioma cell lines U87MG and U251.

[0097] This example illustrates the overexpression of ERRα involved in the present invention mediated by the specific inverse agonist XCT790 or ERRα shRNA and lentivirus, and its effect on the proliferation of glioma cell lines U87MG and U251. The results show that XCT790 and ERRα shRNA down-regulate ERRα It can inhibit the proliferation of glioma cells, and the overexpression of ERRα mediated by lentivirus can promote the proliferation of glioma cells, indicating that the ERRα involved in the present invention shows an extremely important key effect in the in vitro proliferation of human glioma cells. The CCK-8 cell proliferation assay is a technique familiar to those skilled in the art.

[0098] 1. U87MG and U251 cell culture and seeding 96-well cell culture plate:

[0099] Take out the U87MG and U251 in the ...

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Abstract

The present invention discloses use of estrogen-related receptor alpha (ERR alpha) as a diagnostic marker for glioma and related application, also discloses application of the ERR alpha and an activecomponent for down-regulating or blocking of expression of ERR alpha molecule in the glioma in preparation of a related kit and drug for diagnosis and treatment of the glioma, and provides a new method for diagnosis and treatment of the glioma.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and tumor prevention and treatment, in particular to the use of estrogen-related receptor α as a diagnostic marker for glioma and related applications. Background technique [0002] Glioma is the most common intracranial malignant tumor. The incidence of glioma in China accounts for 35% to 60% of intracranial tumors. According to the WHO grading system, glioma is divided into WHO grade Ⅰ-Ⅳ, WHO grade Ⅰ and grade Ⅳ. WHO grade Ⅱ belongs to low-grade glioma, while WHO grade Ⅲ and WHO grade Ⅳ belong to high-grade glioma. In recent years, the incidence of glioma has been increasing year by year, with high malignancy, high recurrence rate and high mortality rate. Currently, The overall treatment effect of glioma is not optimistic. Except for a small number of patients with low-grade glioma (such as pilocytic astrocytoma) who can be cured by surgery, most patients with glioma are still very po...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574G01N33/68A61K45/00A61P35/00
Inventor 李群益张留弟祝迎锋施孝金陈海飞周玉付陈璐戚慧洁任国强唐剑敏钟明康
Owner 复旦大学附属华山医院北院
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