Glioma prognostic marker circ15:98707562|98708107 and applications

A glioma and prognosis technology, applied in the application of circRNA markers, and the application field of reagents in the preparation of glioma prognosis preparations, can solve the problems of the survival rate of glioma patients is not significantly improved, and the survival rate is not optimistic.

Inactive Publication Date: 2018-01-19
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not been significantly improved.
The diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information, and once diagnosed, most of them are in the middle and advanced stages, and the survival rate after surgery is not optimistic

Method used

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  • Glioma prognostic marker circ15:98707562|98708107 and applications
  • Glioma prognostic marker circ15:98707562|98708107 and applications
  • Glioma prognostic marker circ15:98707562|98708107 and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of reagents for detecting the expression of circRNA circ15:98707562|98708107 for the preparation of a kit for the prognosis of glioma patients (50 reactions)

[0019] 1. RNA stabilization solution 50ml

[0020] 2. Isopropanol 100ml

[0021] 3. Chloroform 100ml

[0022] 4. Trizol (from Molecular Research Center company) 50ml

[0023] 5. Enzyme-free water 10ml

[0024] 6. 1μM random reverse transcription primer (Thermo Company) 50μl

[0025] 7. 5× reverse transcription buffer (Thermo company) 200ml

[0026] 8. 10mM base triphosphate deoxynucleotide (Thermo company) 100μl

[0027] 9. 40U / μl RNase inhibitor (Thermo Company) 500μl

[0028] 10. 200U / μl MMLV reverse transcriptase (Thermo Company) 50μl

[0029] 11. Premix Ex Taq (Thermo Company) 50μl

[0030] 12. 10μM circRNA circ15:98707562|98708107 real-time fluorescence quantitative PCR specific primer 30μl

[0031] circRNA circ15:98707562|98708107 Forward primer: 5'-CCGATGTGTGAGAAGACCA-3',

...

Embodiment 2c

[0036] Example 2 The relationship between the expression level of circRNA circ15:98707562|98708107 in glioma tissue and prognosis

[0037] 1. Preservation of glioma tissue: collect the glioma tissue to be tested and store it in a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use.

[0038] 2. Extraction of RNA in tissues: Take an appropriate amount of specimen, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours, grind the specimen, grind to powder, add 1ml Trizol mortar specimen to the mortar, and grind into After the liquid was transferred to a tube tube, it was lysed on ice for 15 minutes. After the lysis, centrifuge at 12000rpm for 10min at 4°C, and transfer the supernatant to a new tube. Add 200μl of chloroform to the tube, shake it by hand for 15-30s, place on ice for 15min, centrifuge at 12000rPm at 4°C for 15min; carefully take the upper aqueous phase into a new tube, add 0.5ml of pre-cooled...

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Abstract

The invention belongs to the biotechnical field, and discloses a glioma prognostic marker circ15:98707562|98708107 and applications, namely a reagent for detecting the expression quantity of circRNA circRNA circ15:98707562|98708107; and the glioma prognostic marker is used for preparing a prognostic preparation for patients with glioma. Study proves that patients with glioma having higher expression quantity of circ15:98707562|98708107 have higher postoperative survival rate. The prognostic prediction on the patients with glioma can be made by detecting the expression level of circRNA circ15:98707562|98708107 in glioma tissues of patients with glioma.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the application of a brain tissue-derived circRNA marker for glioma prognosis, that is, the application of a reagent for detecting the marker in the preparation of a glioma prognosis preparation. Background technique [0002] Glioma is the most frequent brain tumor in adults, accounting for 40.49% of intracranial tumors. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. In order to diagnose and treat glioma, a malignant disease highly correlated with genetic material, it is necessary to explore its pathogenesis at the level of molecular biology and from the expression of genetic information. At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not improved significantly. The diagnosis of glioma is still in the empirical stage based o...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
Inventor 武明花许梓豪李沛瑶王维蒋健晖冯剑波付海娟
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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