Method for extracting macromolecular barley beta-glucan with assistance of centrifugal vortex wall-breaking way

A High Molecular Weight, Extraction-Assisted Technology

Inactive Publication Date: 2018-01-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the extraction of barley β-glucan, the reported extraction methods were initially based on the extraction methods of oat glucan, including hot water extraction, cold water extraction, acid-base extraction, and enzymatic hydrolysis. The time is longer, and the molecular chain of barley β-glucan is partially degraded during the extraction process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) The barley is husked and crushed to pass through a 60-mesh sieve to obtain barley flour;

[0053] (2) Accurately weigh 100g of barley flour, add 1L of 85% ethanol preheated to 80°C, reflux to inactivate the enzyme and degrease for 2h, cool to room temperature, centrifuge at 4000g for 30min, take the precipitate and dry it at room temperature;

[0054] (3) Add the enzyme-inactivated and degreased barley flour into distilled water preheated at 55°C with a solid-to-liquid ratio of 1:10, and adjust the pH value to 7.0;

[0055] (4) Adjust the speed of the colloid mill to 5000r / min, and the gap between the fixed gear and the rotating gear to be 2mm. Put the prepared materials in step (3) into the colloid mill and homogenize for 15 minutes to obtain barley β-glucan Extraction solution;

[0056] (5) Centrifuge the barley β-glucan extract at 8000g for 30min, take the supernatant and preheat it to 95°C, adjust the pH value to 6.0, and add high-temperature-resistant α-amylas...

Embodiment 2

[0062] (1) The barley is husked and crushed to pass through a 60-mesh sieve to obtain barley flour;

[0063] (2) Accurately weigh 500g of barley flour, add 5L of 85% ethanol preheated to 80°C, reflux to inactivate the enzyme and degrease for 2h, cool to room temperature, centrifuge at 4000g for 30min, take the precipitate and dry it at room temperature;

[0064] (3) Add the enzyme-inactivated and degreased barley flour into distilled water preheated at 55°C with a solid-to-liquid ratio of 1:15, and adjust the pH value to 7.0;

[0065] (4) Adjust the speed of the colloid mill to 5000r / min, and the gap between the fixed gear and the rotating gear is 1.5mm. Put the prepared materials in step (3) into the colloid mill and homogenize for 20min to obtain barley β-glucan sugar extract;

[0066] (5) Centrifuge the barley β-glucan extract at 8000g for 30min, take the supernatant and preheat it to 95°C, adjust the pH value to 6.0, add high-temperature-resistant α-amylase at 400u / L (amy...

Embodiment 3

[0072] (1) The barley is husked and crushed to pass through a 60-mesh sieve to obtain barley flour;

[0073] (2) Accurately weigh 1 kg of barley flour, add 10 L of 85% ethanol preheated to 80°C, reflux to inactivate the enzyme and degrease for 2 hours, cool to room temperature, centrifuge at 4000g for 30 minutes, take the precipitate and dry it at room temperature;

[0074] (3) Add the enzyme-inactivated and degreased barley flour into distilled water preheated at 55°C with a solid-to-liquid ratio of 1:15, and adjust the pH value to 7.0;

[0075] (4) Adjust the speed of the colloid mill to 6000r / min, and the gap between the fixed gear and the rotating gear is 1.5mm, put the prepared materials in step (3) into the colloid mill, homogenize for 30min, and obtain barley β-glucan sugar extract;

[0076] (5) Centrifuge the barley β-glucan extract at 8000g for 30min, take the supernatant and preheat it to 95°C, adjust the pH value to 6.0, add high-temperature-resistant α-amylase at ...

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Abstract

The invention discloses a method for extracting macromolecular barley beta-glucan with the assistance of a centrifugal vortex wall-breaking way. The method adopts barley husked grains as a raw material to be crushed, enzyme deactivated and degreased, wall-breaking extracted, enzymolysed, concentrated, precipitated, vacuum freeze-dried and the like to obtain the macromolecular barley beta-glucan byadopting the method of deactivating endogenesis beta-glucanase in the barley grains, extracting with a neutral solution, and extracting with a centrifugal wall-breaking assistance to shorten the extraction time. By adopting the method of the invention, the extraction time can be effectively shortened, the extraction rate of the obtained macromolecular barley beta-glucan can reach 28.5 percent, the purity can reach 91.6 percent, and the molecular weight can reach 1.8*106Da.

Description

technical field [0001] The invention relates to a method for extracting high-molecular-weight soluble functional dietary fiber, in particular to a method for extracting high-molecular-weight barley β-glucan assisted by centrifugal vortex wall breaking, and belongs to the technical field of plant active substance extraction and preparation methods. Background technique [0002] β-glucan is a linear homopolymer of D-glucopyranose linked by β-(1-3,1-4) glycosidic bonds, usually every two to three consecutive β-(1,4) glycosidic bonds are separated by a β-(1,3) glycosidic bond. Cellotriose units (DP3) and cellotetraose units (DP4) account for about 90% to 95% of the total oligosaccharides in the β-glucan molecule, and the content of oligosaccharides with a degree of polymerization greater than 5 is about 5% to 5%. 10%. Cereal-derived beta-glucans have the same basic structure but different characteristics, such as the presence and amount of long-segment cellulose-like fragments...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
Inventor 周惠明黄泽华朱科学郭晓娜彭伟
Owner JIANGNAN UNIV
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