Use Of Ginsenoside M1 For Preventing Or Treating Silicosis
A technology of ginsenoside and silicosis, which is applied in the field of ginsenoside M1 for preventing or treating silicosis, and can solve the problems of reporting the therapeutic efficacy of ginsenoside M1
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[0033] Ginsenoside M1 is known as one of metabolites of protopanaxadiol-type ginsenosides, which are produced via the gypenoside pathway of human intestinal bacteria. After the human body takes ginseng, ginsenoside M1 can be found in blood or urine. Ginsenoside M1 can be prepared from ginseng plants by existing methods in the art such as fungal fermentation, for example, China Taiwan Invention Patent Application No. 094116005 (I280982) and US Patent No. 7,932,057, the entire contents of which are incorporated herein This case is used as reference data. In some specific embodiments, the ginseng plants used to prepare ginsenoside M1 include Araliaceae (Araliaceae), Panax genus (Panax genus), such as ginseng (P.ginseng) and pseudo-ginseng (P.pseudo-ginseng) (also known as Panax notoginseng). Generally speaking, the preparation method of ginsenoside M1 comprises the steps of: (a) providing powder of ginseng plant material (such as leaf or stem); (b) providing fungi for fermentat...
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[0046] 1. Materials and methods
[0047] Ginsenoside M1, namely 20-β-D-glucopyranosyl protopanaxadiol (20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol) (hereinafter named as LCHK168), is based on existing prepared by the method, as disclosed in Chinese Taiwan Patent Application No. 094116005 (I280982) and US Patent No. 7,932,057.
[0048] Mouse macrophage cell line J774A.1 and human monocytogenes and leukocyte cell line THP-1 were purchased from the American Type Culture Collection (Rockville, MD) and cultured in the addition of 10% fetal bovine serum deactivated by high temperature and 2mM L- Glutamine-based RPMI 1640 medium (both purchased from Life Technologies, Carlsbad, CA) was incubated at 37°C, 5% CO 2 in the incubator. To induce differentiation of monocytes into macrophages, THP-1 was cultured in RPMI-1640 for 48 hours with the addition of 100 nM phorbol 12-myristate 13-acetate (Sigma-Aldrich).
[0049] figure 1 Medium, J774A.1 macrophages (1x10 5 cells / 500 μl cultu...
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