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Detection method for phosphatidylserine content
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A phosphatidylserine and detection method technology, which is applied in the field of detection of phosphatidylserine content, can solve problems such as poor peak shape and long retention time, and achieve the effects of changing peak time, shortening retention time and high sensitivity
Active Publication Date: 2018-02-13
WEIHAI BAIHE BIOTECH
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[0004] In order to overcome the shortcomings of too long retention time and poor peak shape in existing detection methods, the purpose of the present invention is to provide a detection method for fatty acylserine content with better phosphorus chromatographic peak separation, accuracy, rapidity and high sensitivity
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Embodiment 1
[0064] A method for detecting the content of phosphatidylserine, which undergoes the following steps:
[0066] Accurately weigh 120mg of the standard sample phosphatidylserine (PS) and dissolve it in 25mL of chloroform / methanol (90 / 10) solvent to make a standard solution, ultrasonically dissolve the powder fully, store it at 4°C, and then store it at 10, 15, 20, 25ul and 30ul standard solutions were injected to make a standard curve;
[0067] (II) Sample solution preparation:
[0068] Accurately and repeatedly weigh a certain amount of commercially available phosphatidylserine-containing samples (a manufacturer of phosphatidylserine-containing formula milk powder), add it to a 50ml volumetric flask, add 10ml of chloroform / methanol (90 / 10), vortex After oscillating to disperse evenly, sonicate for 5 minutes, cool to room temperature, make up to 50mL with chloroform / methanol solution, shake well, prepare sa...
Embodiment 2
[0081] A method for detecting the content of phosphatidylserine, which undergoes the following steps:
[0082] (1) standard solution preparation and drawing standard curve: with embodiment 1;
[0083] (II) Sample solution preparation:
[0084] Accurately and repeatedly weigh a certain amount of commercially available phosphatidylserine-containing samples (phosphatidylserine tablet candy from a certain manufacturer), first grind the tablet candy to a fine powder state, then add it to a 50ml volumetric flask, add 10ml Chloroform / methanol (90 / 10), vortex to disperse evenly, sonicate for 5 minutes, cool to room temperature, make up to 50mL with chloroform / methanol solution, shake well, prepare sample solution, filter through 0.45um microporous membrane, enter Sample;
[0085] (III) Injection detection:
[0086] The standard solution and sample solution are measured under the above-mentioned specific chromatographic conditions, and the retention time of the sample during the elu...
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technical field [0001] The invention relates to testing materials by using adsorption, such as chromatography, to separate materials into components, especially a detection method for detecting phosphatidylserine content in raw materials and finished products. Background technique [0002] As a new food raw material, phosphatidylserine is gradually applied to various foods and health products due to its unique brain-building and intellectual-improving effects. The detection method is stipulated, which leads to no unified basis for the quality control of raw materials containing phosphatidylserine and the quality control of finished products. [0003] At present, there are two published detection methods for phosphatidylserine. The first one is: "a high-efficiency liquid for phosphatidylserine" disclosed in the Chinese invention patent application specification published on February 3, 2016 with the application number: 201510874442.3 Phase chromatographyanalysis method”; th...
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