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Enzymatic extraction method of serotonin

An extraction method, serotonin technology, applied in the field of natural substance extraction, can solve the problems of restricting development and application, and achieve the effects of high extraction efficiency, promotion of recycling, and low impurity content

Inactive Publication Date: 2018-03-02
CHENGDU SHENGERJIA SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no mature method for extracting serotonin from banana peels in the prior art, which limits its development and application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This example provides a method for enzymolysis and extraction of serotonin. This method uses complex enzymes to extract serotonin from banana peels by enzymolysis, and extracts serotonin with high efficiency. It provides the basis for a wide range of applications. The enzymatic extraction method of serotonin provided in this embodiment includes the following steps:

[0038] Step 1: Take fresh banana peels, add drinking water according to the mass ratio of banana peels to water of 1:4, crush and make pulp to obtain slurry.

[0039] Step 2: Add compound enzyme to the slurry for enzymolysis, the compound enzyme is composed of lipase, papain, phytase cellulase and hemicellulase with a mass ratio of 1:0.4:0.4:0.1:0.1, adding The mass concentration of the compound enzyme in the dispersion liquid is 1‰, the temperature is kept at 35°C, the enzymolysis is protected from light for 8 hours, and then the temperature is raised to 80°C to stop the enzymolysis to obtain the enzymoly...

Embodiment 2

[0044] This example provides a method for enzymolysis and extraction of serotonin. This method uses complex enzymes to extract serotonin from banana peels by enzymolysis, and extracts serotonin with high efficiency. It provides the basis for a wide range of applications. The enzymatic extraction method of serotonin provided in this embodiment includes the following steps:

[0045] Step 1: Take fresh banana peels, add drinking water according to the mass ratio of banana peels to water as 1:5, crush and make pulp to obtain slurry.

[0046] Step 2: Add compound enzyme to the slurry for enzymolysis. The compound enzyme is composed of lipase, papain, phytase cellulase and hemicellulase with a mass ratio of 1:0.5:0.3:0.1:0.1. The mass concentration of the compound enzyme in the dispersion is 4‰. Before the compound enzyme is added to the dispersion, disperse with warm water at 40°C and keep it warm for 30 minutes. Keep the temperature at 40° C., protect from light for 6 hours, and...

Embodiment 3

[0051]This example provides a method for enzymolysis and extraction of serotonin. This method uses complex enzymes to extract serotonin from banana peels by enzymolysis, and extracts serotonin with high efficiency. It provides the basis for a wide range of applications. The enzymatic extraction method of serotonin provided in this embodiment includes the following steps:

[0052] Step 1: Take fresh banana peels, add drinking water according to the mass ratio of banana peels to water as 1:6, crush and make pulp to obtain slurry.

[0053] Step 2: Add compound enzyme to the dispersion liquid for enzymolysis. The compound enzyme is composed of lipase, papain, phytase cellulase and hemicellulase with a mass ratio of 1:0.6:0.3:0.2:0.2. Add The mass concentration of the compound enzyme in the dispersion liquid is 8‰. Before adding the compound enzyme into the dispersion liquid, disperse it with warm water at 50°C and keep it warm for 10 minutes. Keep the temperature at 45° C., prot...

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PUM

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Abstract

The invention discloses an enzymatic hydrolysis extraction method of serotonin, and relates to the technical field of extraction of natural substances. The enzymolysis extraction method of serotonin of the present invention comprises: taking fresh banana peel, adding drinking water to crush and pulp to obtain slurry; Filtrate; add ammonium sulfate solution in the first filtrate, leave standstill to precipitate, filter and get the second filtrate; add dehydrated alcohol to its volume percentage in the second filtrate to be 40-50% in the second filtrate, leave standstill to precipitate , and the precipitate was collected by filtration; the precipitate was washed with absolute ethanol and ether, respectively. The enzymolysis extraction method of serotonin provided by the invention is simple and easy, adopts compound enzyme to carry out enzymolysis extraction on banana peel, extracts serotonin with high efficiency, provides a new way for the recovery and utilization of banana peel, and provides serotonin provides the basis for a wide range of applications.

Description

technical field [0001] The invention relates to the technical field of natural substance extraction, in particular to an enzymatic extraction method of serotonin. Background technique [0002] Banana is one of the four major fruits in the world, the most traded fruit in the world, and the most consumed fruit in the world. In China, bananas are the fourth largest fruit after apples, grapes and citrus. Because bananas are not resistant to storage and transportation, they often cause serious losses after harvest. Therefore, some major banana-producing countries have established deep-processing enterprises in the production areas to produce banana food to reduce losses. If it is discarded as waste in the field, it will waste resources and pollute the environment. At the same time, it will also spread some latent germs, which will have a negative impact on production. Therefore, the development and utilization of food by-products and wastes such as banana peels can not only pro...

Claims

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Application Information

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IPC IPC(8): C12P17/10
CPCC12P17/10
Inventor 卢晔
Owner CHENGDU SHENGERJIA SCI & TECH