A kind of application based on non-host plant volatiles as tea geometrid attractant

A technology of host plants and volatiles, applied in the direction of attracting pests, plant growth regulators, chemicals for biological control, etc., to achieve broad industrialization prospects, low cost, and environmental friendliness

Active Publication Date: 2020-05-26
XINYANG AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few domestic research reports on the technology of attracting or repelling tea geometrids

Method used

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  • A kind of application based on non-host plant volatiles as tea geometrid attractant
  • A kind of application based on non-host plant volatiles as tea geometrid attractant
  • A kind of application based on non-host plant volatiles as tea geometrid attractant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 : Extraction of volatiles

[0019] The test plants Nepeta chinensis and Artemisia annua were collected from Zhenlei Mountain in Xinyang and around the campus of Xinyang Agriculture and Forestry University respectively. Wash the test plants, cut them into pieces, dry them naturally, cut them into small pieces, put them in a steam distiller to distill and extract the volatile oil, and put the extracted volatile oil in a -4°C refrigerator for sealed storage for later use.

Embodiment 2

[0020] Example 2 : Antennapotential EAG responses of tea geometrid adults to volatiles

[0021] Pests tested: Tea geometrid larvae were collected from tea gardens around Ma'anshan, Xinyang, brought back to the laboratory and placed in an insect hood, where they were reared continuously for multiple generations. Insert the collected tea leaves with branches into the triangular bottle for 1-2 instar larvae to eat. When the larvae grow to 3-4 instar, they will be reared in an insect breeding box, and a layer of filter paper will be laid in the insect raising box to keep it moist and be replaced regularly. Fresh tea leaves and filter paper. The breeding temperature is 25±1°C, the relative humidity is 60%±5%, and the photoperiod is 16L:8D. After the larvae had pupated and emerged, the males and females were separated, fed with honey water with a volume concentration of 5%, and healthy adults 2-3 days after emergence were selected as test insects.

[0022] Test method: The anten...

Embodiment 3

[0029] Example 3 : Behavioral responses of tea geometrid adults to volatiles.

[0030] The "Y" type olfactometer was used to measure the chemotaxis response of tea geometrid adults to volatiles. During the olfactory measurement, the outlet of the atmospheric sampling bottle was used as the air source, and the air flow of each arm was controlled at 400 mL / min by the LZB-4 glass rotameter. Use a micro sampler to draw 5 μL of the substance solution to be tested, drop it onto a 2cm x 2cm filter paper strip, and put it into the side arm of the "Y" tube. Add acetone dropwise to the filter paper strip of the same specification, and put it into the other arm of the "Y" tube as a control.

[0031] The adults of tea geometrid were more active in the first half of the night, and the experiment was carried out from 18:00 to 23:00. The indoor temperature was kept at (25±1)℃ and the humidity was (60±5)%. 24 hours after the first eclosion, the female and male adults of tea geometrid with...

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PUM

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Abstract

The invention belongs to the technical field of green pest control performed by utilizing semiochemicals and in particular relates to application of volatile matters based on non-host plants serving as a tea geometrid attractant. The application of volatile matters based on non-host plants serving as the tea geometrid attractant is characterized in that the volatile matters based on non-host plants refer to chenopodium ambrosioides volatile matters or artemisia annua volatile matters, or mixtures of the two kinds of volatile matters. The tea geometrid attractant disclosed by the invention hasthe characteristics of being low in cost, high in efficiency, environment-friendly, safe to people and livestock and the like, is particularly suitable for popularization and application of tea growers and has excellent economic, social and ecological benefits and wide industrialization prospects.

Description

technical field [0001] The invention belongs to the technical field of pest green prevention and control by using chemical semiotics, and specifically relates to the use of non-host plant volatiles as an attractant for tea geometrids. Background technique [0002] Tea geometrid belongs to Lepidoptera and Geometridae, and it is an important pest of tea trees in the southeast tea region of my country. Leaf-eating larvae are the main damage mode, and all leaves and buds of tea trees can be eaten up in outbreaks. At present, the main method of controlling tea inchworm is still using organic pesticides. However, the large-scale use of organic pesticides over the years has brought about consequences such as the reduction of natural enemies, the enhancement of pest resistance, environmental pollution, and excessive pesticide residues in tea products. As far as China's tea industry is concerned, it is urgent to adopt biological control technology to reduce the use of pesticides. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/12A01N25/18A01P19/00
CPCA01N25/18A01N65/00A01N65/12
Inventor 张方梅潘鹏亮乔利金银利洪枫陈俊华
Owner XINYANG AGRI & FORESTRY UNIV
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