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Method special for rapid massive extraction of oral epithelial cell DNA

A kind of oral epithelial cells, a large number of technologies, applied in the field of molecular biology experiments, can solve the problems of high cost, long time, and high cost of magnetic beads, and achieve the effects of reducing experimental costs, increasing the amount, and shortening the DNA extraction time

Inactive Publication Date: 2018-03-13
TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the magnetic beads that specifically adsorb DNA cannot be reused, and their cost is relatively high, the cost of the magnetic bead method is high
Moreover, the operation steps of these two methods are relatively cumbersome and take a long time.

Method used

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  • Method special for rapid massive extraction of oral epithelial cell DNA
  • Method special for rapid massive extraction of oral epithelial cell DNA
  • Method special for rapid massive extraction of oral epithelial cell DNA

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Experimental program
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Embodiment Construction

[0028] The present invention will be further described in detail below in conjunction with the accompanying drawings and through specific embodiments. The following embodiments are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0029] A method specially used for rapidly extracting a large amount of oral epithelial cell DNA, the steps are as follows:

[0030] ⑴Collection of sample cells

[0031] Use 2 oral swabs to scrape the oral epithelial cells on the left and right sides of the oral cavity, and transfer the swab heads to 2 1.5ml centrifuge tubes containing 1ml deionized water. Vibrate the centrifuge tube to remove as many cells as possible from the swab, then centrifuge two 1.5ml tubes containing oral epithelial cells at 800×g for 2 minutes. Discard the supernatant, add 500 μl sterile water to each EP tube, transfer the cells in one EP tube to another EP tube, and centrifuge at 800×g for 2 minutes.

[0032] ⑵ lysed cel...

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PUM

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Abstract

The invention relates to a method special for rapid massive extraction of oral epithelial cell DNA, wherein the method comprises the following steps: (1), collecting sample cells; (2) cracking the cells; (3) removing proteins; (4) precipitating DNA; (5) washing with ethanol; (6) dissolving the DNA, and (7) preserving the DNA, marking the DNA sample, placing the DNA sample in a specified position at the temperature of -20 DEG C or -80 DEG C, and preserving. Compared with a centrifugal column method and a magnetic bead method, the method provided by the invention greatly reduces the experimentalcost; compared with the centrifugal column method and the magnetic bead method, the method has the DNA extraction time greatly shortened; compared with the centrifugal column method, the method greatly increases the amount of DNA extraction. The purity of the DNA is within a reasonable range. The extracted genomic DNA does not affect downstream molecular experiments.

Description

technical field [0001] The invention belongs to the field of molecular biology experiments, in particular to a method specially used for rapidly and massively extracting oral epithelial cell DNA. Background technique [0002] Genetic testing is a technology that detects DNA by extracting blood, other fluids or tissue cells from the subject. At present, with the development of biotechnology, genetic testing has gradually become an emerging means of disease and health testing in addition to traditional physical examination. [0003] Deoxyribonucleic acid (DNA) extraction is a basic step in genetic testing and the basis of the entire genetic testing. The concentration and purity of nucleic acid are directly related to the success of subsequent testing. Compared with traditional physical examination, genetic testing can directly extract the DNA of the oral epithelial cells of the subject, which has the advantage of being painless and non-invasive. Therefore, the extraction of ...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 孙麟马洁张同
Owner TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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