Fecal sample processing, fecal occult blood detection method and kit
A kit and feces technology, applied in the field of intestinal disease detection, can solve the problems of reducing enthusiasm and preventing the widespread application of fecal occult blood detection, and achieve the effects of improving detection sensitivity, simplifying sampling procedures, and reducing disgust and repulsion
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Embodiment 1
[0092] In order to determine whether the protein liquid itself affects the results of fecal occult blood detection, the inventors conducted tests under different blood concentrations and milk powder concentrations. Take human blood, milk powder and 10ml of purified water, prepare mixtures containing various concentrations of human blood and milk powder according to Table 1 below, and then use FIT test paper for testing. The test results are shown in Table 1.
[0093] Table 1. Effect of different milk powder and blood concentration on FIT test results
[0094]
[0095] In the above table, if no blood is detected with the FIT test strip, the result is negative, otherwise, if blood is detected, the result is positive.
[0096] It can be seen from the data in Table 1 that the presence of milk fluid does not have any adverse effect on the results of fecal occult blood testing.
Embodiment 2
[0098] In order to further determine whether the protein liquid itself affects the results of fecal occult blood detection, the inventor further conducted tests under different blood concentrations and soybean meal concentrations. Take human blood, soybean powder and 10ml of purified water, prepare mixtures containing various concentrations of human blood and soybean powder according to Table 2 below, and then use FIT test paper for testing. The test results are shown in Table 2.
[0099] Table 2. Effect of different soybean meal and blood concentration on FIT test results
[0100]
[0101] In the above table, the same as in Example 1, if no blood is detected with the FIT test strip, the result is expressed as negative, otherwise, if blood is detected, the result is expressed as positive.
[0102] It can be seen from the data in Table 2 that the presence of soy milk has no adverse effect on the results of fecal occult blood detection.
Embodiment 3
[0104] In order to determine the effect of different concentrations of protein powder on the detection results of fecal occult blood in the presence of toilet paper, the inventors further tested the detection results of fecal occult blood in the presence of toilet paper and milk powder of various concentrations in the extract. Take human blood, milk powder and 10ml of pure water, prepare a mixture containing various concentrations of human blood and milk powder according to Table 3, put it into toilet paper (double-layer, size 10x20cm), stir for 3 minutes, and then use FIT detection Dipsticks for testing. The test results are shown in Table 3 below.
[0105] Table 3. Detection results in the presence of toilet paper and various concentrations of milk powder
[0106]
[0107] In the above table, the same as in Example 1, if no blood is detected with the FIT test strip, the result is expressed as negative, otherwise, if blood is detected, the result is expressed as positive....
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