Sowing method for promoting germination of Paphiopedilum seeds in original place

A technology of native soil and seeds, applied in botany equipment and methods, planting substrates, germination equipment, etc., can solve the problem of restricting the in-depth development of Paphiopedilum plant conservation research, affecting germination fungi and the acquisition and success rate of fungi at each growth stage Low-level problems, easy to recycle and observe, convenient to observe the dynamic development process of embryos, and occupy a small space

Active Publication Date: 2021-02-26
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In conclusion, at present, the success rate of seed germination and seedling development in the native place of Paphiopedilum is low, and the effect is poor, which affects the acquisition of germination fungi and fungi at each growth stage, and limits the in-depth development of research on the conservation of Paphiopedilum species

Method used

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  • Sowing method for promoting germination of Paphiopedilum seeds in original place
  • Sowing method for promoting germination of Paphiopedilum seeds in original place

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034](1) Artificial pollination

[0035]Select robust and good-character flowering plants of Paphiopedilum fortunei for artificial cross-breeding. After 150 days of pollination, pick up mature pods with full morphology for later use.

[0036](2) Seed clip making

[0037]Use nylon cloth with 50μm pore size and cut it into a 22×20cm rectangle. Lay the cut nylon cloth flat and fold it in half along the outer sides of the long side to the center line to obtain a new 20×11cm rectangle, and then follow the long sides of the new rectangle. The outer sides of the two ends are 0.5cm, 4.5cm, 5.5cm, and 9.5cm respectively. 4 edges are heat-sealed. A total of 8 edges are sealed on both sides. Then there will be 8 5.5×4cm rectangular small rooms, two rows of upper and lower sides, each There are 4 in a row, and the two rows have opposite openings, and the openings are reserved for planting openings. Use a transparent milk straw with a diameter of 0.3cm and a length of 15.7cm to be folded into a rectangu...

Embodiment 2

[0048](1) Artificial pollination

[0049]The robust and good-character flowering plants of Paphiopedilum were selected for artificial cross-breeding. After 180 days of pollination, the mature pods were picked for later use.

[0050](2) Seed clip making

[0051]Use nylon cloth with 50μm pore size and cut it into a 22×20cm rectangle. Lay the cut nylon cloth flat and fold it in half along the outer sides of the long side to the center line to obtain a new 20×11cm rectangle, and then follow the long sides of the new rectangle. The outer sides of the two ends are 0.5cm, 4.5cm, 5.5cm, and 9.5cm respectively. 4 edges are heat-sealed. A total of 8 edges are sealed on both sides. Then there will be 8 5.5×4cm rectangular small rooms, two rows of upper and lower sides, each There are 4 in a row, and the two rows have opposite openings and reserved as sowing openings. Use a transparent milk straw with a diameter of 0.4cm and a length of 15.7cm to be folded into a rectangular frame of 4.5×3.5cm, with the...

Embodiment 3

[0062](1) Artificial pollination

[0063]The robust and good-character flowering Paphiopedilum species were selected for artificial cross-breeding. After 210 days of pollination, the mature fruit pods were picked for later use.

[0064](2) Seed clip making

[0065]Cut the nylon cloth with a hole diameter of 48μm into a 22×20cm rectangle, lay the nylon cloth flat and fold it in half along the outer sides of the long side toward the center line to obtain a new 20×11cm rectangle, and then follow the long sides of the new rectangle. The outer sides of the two ends are 0.5cm, 4.5cm, 5.5cm, and 9.5cm respectively. 4 edges are heat-sealed. A total of 8 edges are sealed on both sides. Then there will be 8 5.5×4cm rectangular small rooms, two rows of upper and lower sides, each There are 4 in a row, and the two rows have opposite openings, and the openings are reserved for planting openings. Use a transparent milk straw with a diameter of 0.3cm and a length of 15.7cm to be folded into a rectangular b...

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Abstract

The invention discloses a paphiopedilum seed clip, which is made of square nylon cloth, and the square nylon cloth has two rows of upper and lower seed chambers with opposite openings. Accordingly, the inventor has also established a corresponding sowing method for promoting the germination of Paphiopedilum seeds in the original place. The above-mentioned seed clips are used to sow 150-210d Pachyphytum seeds. Sowing in the original place. In a word, the operation method of the present invention is simple, flexible, high germination rate, well-developed seedlings, convenient to observe the dynamic development process of seed embryos, easy to separate mycorrhizal fungi in different stages, and reduce the impact of multiple observations or material collection on other seed embryos. At the same time, it is suitable for simultaneous sowing of multiple Paphiopedilum seeds, and opens up a new sowing method for conservation research such as reintroduction and symbiotic germination of endangered Paphiopedilum.

Description

Technical field[0001]The invention belongs to the technical field of Paphiopedilum seed germination, and particularly relates to a sowing method for promoting the germination of Paphiopedilum seeds in their native place.Background technique[0002]Paphiopedilum is one of the most popular orchid plants. It is a valuable ornamental orchid widely cultivated all over the world. Due to excessive man-made excavation and habitat destruction, the number of wild paphiopedilum resources is rapidly decreasing, and the distribution area is gradually shrinking. Critically endangered, all species of this genus are listed in Appendix I of the Convention on International Trade (CITES).[0003]Like other orchids, Paphiopedilum has a large number of small seeds, but the endosperm of the seeds is missing. Under natural conditions, Paphiopedilum seeds can only germinate normally with symbiosis with fungi, and the germination rate is very low. At present, tissue culture of Paphiopedilum is still very diffic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/02A01G31/00A01G24/28A01G24/23A01G24/12
CPCA01C1/02A01G31/00
Inventor 陈宝玲王华新龚建英周千淞唐遒冥唐庆龙定建陈尔苏莉花杨舒婷林茂
Owner GUANGXI FORESTRY RES INST
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