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Bacillus coagulans E21 capable of improving oxidative stress resistance of livestock and poultry animals after toxic attack, and application thereof

A Bacillus coagulans, anti-oxidative stress technology, applied in applications, animal feed, animal feed, etc., can solve the problems of anti-bile salt and poor high temperature resistance, and achieve good effect and good heat resistance

Active Publication Date: 2018-04-03
QINGDAO GENYUAN BIOLOGICAL TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, probiotics widely used at home and abroad include Bacillus, Lactobacillus, Bifidobacterium and yeast, etc., but most lactic acid bacteria have poor resistance to gastric acid, bile salts and high temperature, while Bacillus has strong stress resistance, High temperature and high pressure resistance, easy storage and other advantages have become a research hotspot in probiotics

Method used

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  • Bacillus coagulans E21 capable of improving oxidative stress resistance of livestock and poultry animals after toxic attack, and application thereof
  • Bacillus coagulans E21 capable of improving oxidative stress resistance of livestock and poultry animals after toxic attack, and application thereof
  • Bacillus coagulans E21 capable of improving oxidative stress resistance of livestock and poultry animals after toxic attack, and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] The strain comes from the cecal digesta of healthy chickens. After multiple screening and purification tests, the pure strain of the bacteria can be obtained. The bacteria can produce acid and spores. The DNA of the bacteria is extracted after the pure bacteria are cultured in liquid medium, and then 16S Universal primers were used to amplify the full length of 16S rRNA, and the amplified product was sent to a sequencing company for sequencing. The sequencing results were compared with Bacillus coagulans and found to be 99% similar to Bacillus coagulans, confirming that the bacterium was Bacillus coagulans. The whole genome of the strain was sequenced using the third-generation sequencing PacBio technology, and it was found that the strain was inconsistent with the strains on the market, and was identified as Bacillus coagulans E21.

[0022] The physiological and biochemical properties of the Bacillus coagulans E21 screened by the present invention are as follows: Gram-p...

Embodiment 2

[0032] In this experiment, diquat, a bipyridine herbicide, was used to attack white-feathered broiler chickens. The specific operations are as follows:

[0033] The test selects 1-day-old white-feathered broiler chickens, pre-feeds them for 7 days, removes the chicks with large body weight differences, and the rest are grouped. The test is divided into 3 treatment groups, and each treatment group has 4 repetitions (i.e. 4 stalls). Repeat 15 chickens, and 3 groups are challenged and processed in the following way:

[0034] Control group: without adding any probiotics, on the 21st day of the test, the same amount of normal saline was injected;

[0035] Test group 1: without adding any probiotics, on the 21st day of the test, intraperitoneal injection of diquat (15 mg / kg body weight, diquat dilution concentration of 15 mg / mL);

[0036] Test group 2: Add 10 6 CFU / mL of Bacillus coagulans, on the 21st day of the test, inject diquat (15mg / kg body weight, diquat dilution concentrat...

Embodiment 3

[0061] The Bacillus coagulans obtained in the present invention is fermented and cultivated. The fermentation of the bacteria utilizes a 15L fermenter, the fermentation period is 24-30h, and the amount of fermented bacteria reaches 10 billion CFU / mL, wherein the formula of the fermentation medium is: soybean meal 3-5%, Peptone 1-2%, cornmeal 3-5%, glucose 1-1.5%, dipotassium hydrogen phosphate 0.3-0.5%, manganese sulfate 0.02-0.1%, sodium nitrate 0.05-0.1%. 20 billion CFU / g Bacillus coagulans powder can be obtained after post-treatment and other processes after fermentation. The Bacillus coagulans powder can be used in combination with other probiotics, or used alone in feed, or fed to broilers Drinking water is added during the process and used alone.

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Abstract

The invention provides bacillus coagulans E21 capable of improving oxidative stress resistance of livestock and poultry animals after toxic attack, and the application thereof. The patent preservationnumber of the strain is CGMCC NO.13044. The strain is derived from the intestinal tract of a healthy animal; a series of experiments prove that the strain has high heat resistance, acid resistance, cholate tolerance, stability and capability of producing high-yield L-lactic acid; furthermore, the zymophyte quantity of the bacillus coagulans reaches to 10 billion CFU / mL, and the bacillus coagulanscan be applied to feed additives. The bacillus coagulans can improve the oxidation resistance of white-feather broiler chickens after toxic attack, the white-feather broiler chickens are subjected totoxic attack by aquacide, the organism is in an oxidative stress state, the morphological structure of jejunum is damaged, and the intestinal barrier function is influenced. Due to the addition of the bacillus coagulans, the oxidation resistance of the white-feather broiler chickens after toxic attack can be improved, the morphological structure of the jejunum is recovered, balance of inflammation-causing factors and inflammation-inhibiting factors is promoted, and the intestinal barrier function is protected.

Description

technical field [0001] The invention belongs to the technical field of microbial additives and applications, and in particular relates to a strain of bacillus coagulans E21 that improves the anti-oxidative stress ability of livestock and poultry animals after challenge and its application. Background technique [0002] The intestinal tract is not only the largest digestive and absorption organ in the body, but also an important immune organ in the body. The intestinal mucosa with complete physiological functions forms a barrier to bacteria and endotoxins. However, in a state of stress, a large number of bacteria and toxins enter the host through the gastrointestinal tract, reducing the resistance of intestinal mucosal epithelial cells, destroying tight junctions, and damaging the intestinal mucosal barrier. Therefore, enhancing the immune function and disease resistance of animals and alleviating intestinal damage caused by oxidative stress through nutritional regulation has...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/30A23K50/75C12R1/07
CPCA23K10/18A23K50/30A23K50/75C12N1/205C12R2001/07
Inventor 冯海霞王政张大伟王文博王泽宇张宗国赵志强
Owner QINGDAO GENYUAN BIOLOGICAL TECH GRP
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