Method of extracting and preparing high-purity verbascoside from flowers of Campsis plants

A technology of acteoside and acteoside, which is applied in the field of medicine, can solve the problems of little promotion and application value, cumbersome and complicated operation process, low yield of acteoside, etc., and achieves the advantages of rich drug sources, high yield and purity, and easy promotion Effect

Active Publication Date: 2018-04-06
中国人民解放军陆军第七十一集团军医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for full utilization of natural sources like Camellia sinensis or other plants from this genus called Campbellaceae (Bignonia). It also includes methods that can be easily done at lower costs compared to traditional ways. Additionally, it provides an efficient way to make pure compounds containing these active ingredients without harmful chemicals being added during processing steps. Overall, this process makes better medicine than what was previously possible by creating new ones through chemistry techniques alone.

Problems solved by technology

This patented technical problem addressed in this patent relates to improving the production efficiency and quality control over extracted or prepared vanilloid gamma glucopyranone dihydroxyproline(β)-malonyglutathiononesynthesis with different sources like bergenesscininosilane, lithochlorophyllotensitive receptor protein kinases, carboxysimuthene synthesis pathways, xanthanes, lycophoricospid biosynthetic intermediary polygrosimepodiphosphorus sulfurederivatives, hydroquinazines, coumarnsulfonapy drugs, quaternaries salts, cyclohexadienediones, beta lactobionic esters, pigments, bioactive agents, pesticidal compositions made up of them alone or combined together.

Method used

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  • Method of extracting and preparing high-purity verbascoside from flowers of Campsis plants
  • Method of extracting and preparing high-purity verbascoside from flowers of Campsis plants
  • Method of extracting and preparing high-purity verbascoside from flowers of Campsis plants

Examples

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Embodiment 1

[0028] Example 1: Extracting and preparing high-purity verbascoside from Ling Xiaohua

[0029]500g of Lingxiao dried flower medicinal material, cut into sections, soaked in 5 times the volume of ethyl acetate for 24 hours, extracted by percolation at 20 times the volume, the extract was concentrated under reduced pressure at 65°C until there was no ester smell, and 18g of liquid extract was obtained. 3.6%, retained for the preparation of high-purity ursolic acid. The extracted medicinal material residue was soaked in 5 times the volume of 60% ethanol aqueous solution for 24 hours, 20 times the volume was percolated and extracted, and the extract was concentrated under reduced pressure at 65°C until it had no alcohol taste, and 210g of acteoside liquid extract was obtained, with a yield of 42 %.

[0030] Add 3 times the volume of pure water to completely dissolve the above-prepared acteoside fluid extract, then perform macroporous adsorption resin column chromatography with a ...

Embodiment 2

[0038] Example 2: Extracting and preparing high-purity verbascoside from Lingxiao flower

[0039] 500g of dried flower medicinal material of American Lingxiao, cut into sections, soaked in 5 times the volume of ethyl acetate for 24 hours, heated and refluxed to extract 5 times the volume for 3 times, each time for 1 hour, the extract was concentrated under reduced pressure at 65°C until it had no ester smell, and obtained Fluid extract 21g, yield 4.2%, retained for the preparation of high-purity ursolic acid. Soak the extracted medicinal material residue in 5 times the volume of 60% ethanol aqueous solution for 24 hours, heat and reflux extraction for 3 times, each time for 1 hour, and concentrate the extract under reduced pressure at 65°C until it has no alcohol taste, so as to obtain 240g of verbascoside liquid extract. The rate is 48%.

[0040] Add 3 times the volume of pure water to completely dissolve the above-prepared acteoside fluid extract, then perform macroporous a...

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to a method of extracting and preparing high-purity verbascoside from flowers of Campsis plants. The extraction andpreparation method includes two extraction steps and two column chromatographic steps, comprising: ethyl acetate extraction to remove impurities, extraction with an ethanol water solution to prepare verbascoside fluid extract, column chromatography through a macro-pore adsorption resin column to obtain a verbascoside crude extract, and column chromatography through reverse phase silica gel columnto obtain the high-purity verbascoside. The purity of a final product of verbascoside powder is higher than 98%. The method is used for extracting and preparing the verbascoside by fully utilizing theCampsis plant medicine resources, so that raw material source is developed and is abundant. For the first time, the method employs the two-step extraction and two-step column chromatography, so thatthe extraction and preparation method is optimized and modified, and is simple in operations and low in cost. During operations, only are two organic reagents, ethanol and ethyl acetate, employed without toxic reagents such as methanol, acetone, etc. The method is toxic-free and harmless and green and environment-friendly, is high in yield and purity of the verbascoside, is easy to popularize andis valuable to recommend.

Description

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Claims

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Application Information

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Owner 中国人民解放军陆军第七十一集团军医院
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