Method for rapid detection of impact of acid rain on rice health by means of microbial electrochemical sensor

A microbial electrochemistry and sensor technology, applied in the field of bioelectrochemistry, can solve the problems of high sensitivity, low cost, and difficulty in online continuous detection, and achieve the effect of overcoming slow response.

Active Publication Date: 2018-04-10
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing technology is difficult to meet the requirements of online continuous detection, low cost and high sensitivity, and to provide a method that uses microbial electrochemical sensors to respond to plant health status, in order to provide online, fast and sensitive plant health status method of information

Method used

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  • Method for rapid detection of impact of acid rain on rice health by means of microbial electrochemical sensor
  • Method for rapid detection of impact of acid rain on rice health by means of microbial electrochemical sensor
  • Method for rapid detection of impact of acid rain on rice health by means of microbial electrochemical sensor

Examples

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Effect test

Embodiment 1

[0023] Embodiment 1: A kind of method that the acid rain of pH2.5 rapidly detects the health impact of rice by microbial electrochemical sensor

[0024] Rice seeds were first floated and then treated with 5% H 2 o 2 Sterilize for 30 minutes, then soak the seeds in distilled water for 15 hours, and finally spread the seeds evenly on the gauze tray, seal them, and cultivate them under light. The incubator is set at 25 degrees for 14 hours of light and 10 hours of darkness. After 7 days, the rice was harvested and transferred to the hydroponic equipment for 5 days to prepare for the use of the sensor.

[0025] The size of the sensor container is 15cm in upper diameter, 15cm in lower diameter, and 12cm in height, the material is polytetrafluoroethylene, and the working electrode is 36cm 2 The carbon cloth electrode, the reference electrode is 3.5MAg / AgCl, the counter electrode is 1cm 2 platinum sheet. Such as figure 1 shown.

[0026] First prepare the microbial electrochemic...

Embodiment 2

[0028] Embodiment 2: A kind of method that the acid rain of pH3.5 rapidly detects the health impact of rice by microbial electrochemical sensor

[0029] Rice seeds were first floated and then treated with 10% H 2 o 2 Sterilize for 20 minutes, then soak the seeds in distilled water for 10 hours, finally spread the seeds evenly on the gauze tray, seal them, and cultivate them under light. The incubator is set at 25 degrees for 14 hours of light and 10 hours of darkness. After 5 days, the rice was harvested and transferred to the hydroponic equipment for 6 days to prepare for the use of the sensor.

[0030] The size of the sensor container is 18cm in upper diameter, 15cm in lower diameter, and 15cm in height, the material is polytetrafluoroethylene, and the working electrode is 50cm 2 The carbon cloth electrode, the reference electrode is 4MAg / AgCl, the counter electrode is 1cm 2 platinum sheet.

[0031] First prepare the microbial electrochemical sensor. The solution added t...

Embodiment 3

[0033] Embodiment 3: A kind of method that the acid rain of pH5.5 rapidly detects the health impact of rice by microbial electrochemical sensor

[0034] Rice seeds were first floated and then treated with 15% H 2 o 2 Sterilize for 10 minutes, then soak the seeds in distilled water for 5 hours, finally spread the seeds evenly on the gauze tray, seal them, and cultivate them under light. The incubator is set at 25 degrees for 14 hours of light and 10 hours of darkness. The rice harvested in 6 days was transferred to the hydroponic equipment for 7 days to prepare for the use of sensors.

[0035] The size of the sensor container is 20cm in diameter, 10cm in diameter, and 18cm in height. The material is polytetrafluoroethylene, and the working electrode is 100cm. 2 The carbon cloth electrode, the reference electrode is 3.5MAg / AgCl, the counter electrode is 10cm 2 platinum sheet.

[0036] First prepare the microbial electrochemical sensor, the solution added to the container is ...

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Abstract

Disclosed is a method for rapid detection of an impact of acid rain on rice health by means of a microbial electrochemical sensor. The plant microbial electrochemical sensor is composed of rice and asensor. A container of the sensor is composed of a teflon material with an upper diameter of 15-20 centimeters, a lower diameter of 10-15 centimeters and a height of 12-18 centimeters; a working electrode is carbon electrode with an area of 20-100 square centimeters, a reference electrode is 3.5M/4MAg/AgCl, and a counter electrode is composed of a platinum sheet with an area of 1-10 square centimeters. According to the method, in-situ rapid acquisition of health state of the rice is achieved by measn of of the sensor , and the sensor is stable and accurate in operation, and can timely respondto heath damage of the rice caused by the acid rain with a pH value between 2.5-5.5, thereby achieving the purpose of timely warning; defects of being slow in response, high in costs and incapable ofachieving timely detection of the traditional detection technology are overcome, and a new technology for later detection of the heath state of plants is provided.

Description

technical field [0001] The invention belongs to the technical field of bioelectrochemistry, and in particular relates to a method for on-line detection of rice health status using a bioelectrochemical sensor based on electrogenic microorganisms. Background technique [0002] As one of the most common environmental problems in the world, acid rain has seriously threatened the safety of plants, especially local crops, resulting in reduced photosynthetic efficiency, slow growth and ultimately reduced food production. Rice, as one of the three main crops, with the development of industrialization, its growth is often affected by acid rain, resulting in rice production has been affected. The health of plants such as rice is mainly manifested through the changes of roots, stems, leaves and fruits. In order to prevent the morbid characteristics of plants in advance, it is mainly by observing the characteristics of leaves and stems of plants. However, under the action of acid rain, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N17/02
CPCG01N17/02
Inventor 王鑫李田周启星
Owner NANKAI UNIV
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