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Infusion liquid for repairing of mesenchymal stem cells and preparation method thereof

A technology of high-quality stem cells and injections, applied in the field of injections, can solve the problems of decreased activity of stem cells and inability to guarantee cell activity for a long time, and achieve high survival rate, high stability, and guaranteed stability

Active Publication Date: 2018-04-13
BEIJING ZHEN HUIKANG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, unlike other injections, the active ingredients in mesenchymal stem cell injections are stem cells. Traditionally used to prepare injections such as sodium chloride, DMSO and other auxiliary materials to preserve mesenchymal stem cells will make the activity of stem cells Significant decline, unable to guarantee cell viability for a long time

Method used

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  • Infusion liquid for repairing of mesenchymal stem cells and preparation method thereof
  • Infusion liquid for repairing of mesenchymal stem cells and preparation method thereof
  • Infusion liquid for repairing of mesenchymal stem cells and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 An injection

[0039] The injection is composed of mesenchymal stem cell microspheres and an injection mixture. The mesenchymal stem cell microspheres are composed of mesenchymal stem cells and a microsphere carrier. Each mL of the injection mixture contains 0.5×10 6 Each mesenchymal stem cell contains 2.5g of microsphere carrier prepared from the following weight components:

[0040] Propylene carbonate 0.7g

[0041] Methionine 0.3g

[0042] Pullulan-polyacrylic acid cross-linked 1.5g

[0043] The injection mixture is prepared from the following components by weight:

[0044] Deionized water 8g

[0045] Cocamidopropyl betaine 0.5g

[0046] Chitosan Microcapsules

[0047] Chitosan 1g

[0048] Polyethylene glycol lactate 0.2g

[0049] Polyacrylamide 0.3g.

Embodiment 2

[0050] Example 2 An injection

[0051] The injection is composed of mesenchymal stem cell microspheres and an injection mixture. Among them, the mesenchymal stem cell microspheres are composed of mesenchymal stem cells and a microsphere carrier. Each mL of the injection mixture contains 1×10 6 Each mesenchymal stem cell contains 3.9g of microsphere carrier, which is prepared from the following weight components:

[0052] Propylene carbonate 1g

[0053] Methionine 0.4g

[0054] Pullulan-polyacrylic acid cross-linked 2.5g

[0055] The injection mixture is prepared from the following components by weight:

[0056] Deionized water 10g

[0057] Cocamidopropyl Betaine 0.7g

[0058] Chitosan Microcapsules

[0059] Chitosan 15g

[0060] Polyethylene glycol lactate 0.3g

[0061] Polyacrylamide 0.4g

[0062] The preparation method of the injection is:

[0063] S1: Preparation of mesenchymal stem cell microspheres:

[0064] Preparation of pullulan-polyacrylic acid cross-linked product: configure a pullulan...

Embodiment 3

[0069] Example 3 An injection

[0070] The injection is composed of mesenchymal stem cell microspheres and an injection mixture. The mesenchymal stem cell microspheres are composed of mesenchymal stem cells and a microsphere carrier. Each mL of the injection mixture contains 2×10 6 Each mesenchymal stem cell contains 6g of microsphere carrier prepared from the following components by weight:

[0071] Propylene carbonate 1.5g

[0072] Methionine 0.5g

[0073] Pullulan-polyacrylic acid crosslinked product 4g

[0074] The injection mixture is prepared from the following components by weight:

[0075] Deionized water 12.5g

[0076] Cocamidopropyl Betaine 1g

[0077] Chitosan Microcapsules

[0078] Chitosan 1.9g

[0079] Polyethylene glycol lactate 0.5g

[0080] Polyacrylamide 0.5g.

[0081] The preparation method is the same as in Example 2.

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Abstract

The invention provides an infusion liquid for repairing of mesenchymal stem cells and a preparation method thereof. The infusion liquid consists of mesenchymal stem cell microspheres and an injectionmixing solution, wherein each mesenchymal stem cell microsphere consists of mesenchymal stem cells and a microsphere carrier; each microsphere carrier mainly is prepared from propylene carbonate, methionine and pullulan-polyacrylic acid crosslinking matter; every 1 mL of injection mixing solution contains 0.5*10<6> to 2*10<6> mesenchymal stem cells, and 2.5 to 6g of microsphere carrier. The infusion liquid for the repairing of the mesenchymal stem cells has the advantages that after the mesenchymal stem cells are prepared into the microspheres, and the special injection mixing matter is selected, the stability of the injection liquid is obviously improved; the mesenchymal stem cells can be stored for a long time; the survival rate of the mesenchymal stem cells is higher.

Description

Technical field [0001] The invention belongs to the technical field of injections, and particularly relates to a mesenchymal stem cell repair injection and a preparation method thereof. Background technique [0002] Mesenchymal stem cells are important members of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development. Mesenchymal stem cells were first discovered in the bone marrow. They have multidirectional differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and other functions, so they have attracted much attention. Mesenchymal stem cells can differentiate into adipose, bone, nerve and other tissue cells under induction in vivo or in vitro, and continuous subculture still has the potential for multidirectional differentiation. [0003] Mesenchymal stem cell injection can be used clinically to prevent and treat immune system diseases, diabetes, etc. But unlike other injections, the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/00A61K47/24A61K47/36A61K47/32A61K47/18A61K47/34A61P3/10A61P37/02
CPCA61K9/0019A61K35/28A61K47/183A61K47/24A61K47/32A61K47/34A61K47/36
Inventor 曹毓琳刘俊江时兆田白志惠
Owner BEIJING ZHEN HUIKANG BIOLOGICAL TECH CO LTD