Application of total glycoside from cornus officinalis to preparation of medicine for preventing and curing ventricular remodeling

A technology of ventricular remodeling and dogwood, applied in drug combinations, pharmaceutical formulas, plant raw materials, etc.

Inactive Publication Date: 2018-04-20
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on this, we speculate that the total glucosides of Cornus officinalis may have anti-ventricular remodeling effects, but there are no research reports on the application of total glycosides of cornus officinalis in the treatment of related diseases based on ventricular remodeling

Method used

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  • Application of total glycoside from cornus officinalis to preparation of medicine for preventing and curing ventricular remodeling
  • Application of total glycoside from cornus officinalis to preparation of medicine for preventing and curing ventricular remodeling
  • Application of total glycoside from cornus officinalis to preparation of medicine for preventing and curing ventricular remodeling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Preliminary study on the anti-ventricular remodeling effect and mechanism of total glucosides of Cornus officinalis

[0030] 1 Materials and methods

[0031] 1.1 Animals, reagents and main instruments

[0032]Healthy male Wistar rats with a body mass of (234±22) g were purchased from the Experimental Animal Center of Zhejiang Province, license number: SCXK (Zhejiang) 2015-0004. Total glucosides from cornus officinalis (TGCO), prepared in the laboratory, see the results for the method; rabbit anti-rat p-PKCα polyclonal antibody, purchased from Santa Cruz, USA; horseradish peroxidase-labeled goat anti-rabbit and Rabbit anti-mouse IgG and TGF-β1 monoclonal antibody were purchased from Wuhan Boster Biotechnology Co., Ltd.; enhanced chemiluminescence (ECL) kit was purchased from Yuntian Biotechnology Co., Ltd. Other reagents were commercially available analytically pure . Angiotensin II (Angiotensin II, Ang II) and tumor necrosis factor-α (tumor necrosis factor...

Embodiment 2

[0077] Example 2: Total glucosides of Cornus officinalis inhibits ventricular remodeling in rats after myocardial infarction

[0078] 1 Materials and methods

[0079] 1.1 Animals, reagents and main instruments

[0080] Healthy male Wistar rats, weighing (224±22) g, were purchased from the Experimental Animal Center of Zhejiang Province, license number: SCXK (Zhejiang) 2015-0007. Total glucosides from cornus officinalis (TGCO), prepared in the laboratory, the specific method is the same as above; captopril (captopril, Cap) Sino-US Shanghai Bristol-Myers Squibb Pharmaceutical Co., Ltd., 12.5mg / tablet, batch number 160104. Rabbit anti-rat NF-κB p65 polyclonal antibody; mouse anti-rat MMP-9 monoclonal antibody was purchased from Santa Cruz, USA; horseradish peroxidase-labeled goat anti-rabbit and rabbit anti-mouse IgG were purchased from Wuhan Boster Biotechnology Co., Ltd.; enhanced chemiluminescence (ECL) kit was purchased from Shanghai Biyuntian Biotechnology Co., Ltd.; other...

Embodiment 3

[0108] Example 3: Total glycosides of Cornus officinalis inhibit TGF-β1 / Smad pathway against ventricular remodeling in spontaneously hypertensive rats

[0109] 1. Materials and Methods

[0110] 1.1 Experimental animals, drugs, reagents and instruments

[0111] 40 male spontaneously hypertensive rats (spontaneous hypertensive rats, SHR) and 10 Wistar-Kyoto rats (WKY), 12 weeks old, weighing 210-240 g, were purchased from Shanghai Slack Experimental Animal Co., Ltd., licensed The certificate number is SCXK (Shanghai) 2016-0002. The rats were housed in separate cages and had free access to food (the feed was purchased from the Experimental Animal Center of Zhejiang Province) and water. Total glucosides from cornus officinalis (TGCO), prepared in the laboratory, with the same method as before; Captopril (Cap), Sino-US Shanghai Bristol-Myers Squibb Pharmaceutical Co., Ltd., 12.5mg / tablet, batch number 160104, add appropriate amount of distilled water before use , prepared into a...

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Abstract

The invention provides a total glycoside from cornus officinalis, a medicinal salt of the total glycoside from the cornus officinalis, or an application of a pharmaceutical composition comprising thetotal glycoside from the cornus officinalis or the medicinal salt of the total glycoside from the cornus officinalis and a human body acceptable pharmaceutic adjuvant to the preparation of a medicinefor preventing or curing ventricular remodeling or a diseases based on the ventricular remodeling; the total glycoside from the cornus officinalis is used to preliminarily study the anti-ventricular remodeling action on a mouse and the mechanism of the action, the total glycoside from the cornus officinalis inhibits the ventricular remodeling of the mouse after the myocardial infarction, and inhibits a passage TGF-beta1 / Smad to cope with the spontaneous hypertension mouse ventricular remodeling; the active ingredient of the traditional Chinese medicine, namely the total glycoside from the cornus officinalis, is used at different doses, the pharmacodynamics integral animal experiment study achieves the positive therapeutic effect, and a foundation is provided for screening the new medicine.

Description

(1) Technical field [0001] The invention relates to a new application of total glycosides of cornus, in particular to the application of total glycosides of cornus in the preparation of medicines for preventing and treating ventricular remodeling. (2) Background technology [0002] Cornus officinalis is a perennial small tree or shrub of the genus Cornus officinalis. Cornus officinalis as a traditional Chinese medicinal material is the dried and mature pulp of the dogwood plant Cornus officinalis, also known as shanying meat, dogwood meat, Shuzao, Shuzizi, Kou Si, Siyi, Yingrou, Zaopi, etc. Dogwood resources are widely distributed, and the production areas are mainly concentrated in the Funiu Mountains, Tianmu Mountains and Qinling Mountains in the Yangtze River and Yellow River basins. Tianmu Mountain in Zhejiang is also an important production area of ​​dogwood, mainly including Lin'an, Chun'an, Tonglu and other counties and cities. The flesh of Cornus officinalis is sou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/40A61P9/10A61P9/12A61P9/00A61P11/00A61P3/10A61P7/06
CPCA61K36/40A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 李乐寇文颖张彦军宋坤杰陶厚权
Owner ZHEJIANG UNIV OF TECH
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