Application of gynostemma pentaphylla general flavone in preparation of medicines for preventing and treating cardiac hypertrophy

A technology of myocardial hypertrophy and Gynostemma pentaphyllum, applied in the field of application of total flavonoids of Gynostemma pentaphyllum in the preparation of drugs for preventing and treating myocardial hypertrophy

Active Publication Date: 2017-07-21
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no research reports at home and abroad on the application of total flavonoids of Gynostemma pentaphyllum in the treatment of related diseases based on myocardial hypertrophy

Method used

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  • Application of gynostemma pentaphylla general flavone in preparation of medicines for preventing and treating cardiac hypertrophy
  • Application of gynostemma pentaphylla general flavone in preparation of medicines for preventing and treating cardiac hypertrophy
  • Application of gynostemma pentaphylla general flavone in preparation of medicines for preventing and treating cardiac hypertrophy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Preliminary study on the slowing down of myocardial hypertrophy and its mechanism by total flavonoids of Gynostemma pentaphyllum in spontaneously hypertensive rats

[0047] 1 material

[0048] 1.1 Raw materials and medicines Total flaveos of Gynostemm pentaphyllum (Thunb)

[0049] Makinor, TFG); Captopril (Captopril, Cap), specifications: 25mg / tablet, batch number 20151214, Shantou Jinshi Pharmaceutical General Factory, Guangdong.

[0050] 1.2 Reagents and instruments

[0051] 1.2.1 The main reagent iodine [ 125 I] Angiotensin II ( 125 I-AngiotensinⅡ, 125 I-AngⅡ) radioimmunoassay kit, lot number: 150132; Iodine [ 125 I] Aldosterone 125 Ⅰ-Aldosterone, 125 I-Ald) radioimmunoassay kit, batch number: 150084; both purchased from Beijing North Institute of Biotechnology; TRIzol: Invitrogen, batch number: 15596018; total RNA extraction kit and cDNA first-strand synthesis kit (Hangzhou Bioer Technology Co., Ltd. Company); fluorescent PCR kit (Dalian Baosheng ...

Embodiment 2

[0095] Example 2: TFG inhibits isoproterenol-induced myocardial hypertrophy in mice and its mechanism

[0096] 1 Materials and methods

[0097] 1.1 Experimental animals:

[0098] Clean-grade Kunming mice, both male and female, weighing (20±2) g, provided by Zhejiang Experimental Animal Center, certificate number: SCXK (Zhejiang) 2016-0013, in the barrier environment animal laboratory of Zhejiang Experimental Animal Center Feeding and experimenting.

[0099] 1.2 Drugs and reagents:

[0100] Gymostemma Pentaphyllum (Thunb) Mak (TFG), total flaveos of Gymostemma Pentaphyllum (Thunb) Mak, self-made (see Example 1 for the method); isoprenaline hydrochloride (isoprenaline, Iso) injection (Shanghai Hemu Pharmaceutical Co., Ltd., batch number 160008) ; Lactate dehydrogenase (LDH), malondialdehyde (MDA) and total superoxide dismutase (T-SOD) test box, protein quantitative test box were purchased from Nanjing Jiancheng Biological Engineering Co., Ltd. Rabbit anti-ERK1 / 2 antibody was ...

Embodiment 3

[0131] Example 3: Study on the protective effect and mechanism of TFG on myocardial hypertrophy induced by abdominal aortic constriction in rats.

[0132] lMaterials and methods

[0133] 1.1 Materials

[0134] 1.1.1 Main reagents and instruments

[0135] Total flaveos of Gymostemma Pentaphyllum (Thunb) Mak, TFG, self-made (see Example 1 for the method); Valsartan, China Resources Secco Pharmaceutical Co., Ltd., batch number: 201512008; intracytoplasmic calcium ion concentration Fluorescent detection kit (fura-3 / AM), Shanghai Baoman Biotechnology Co., Ltd.; goat anti-mouse atrial natriuretic peptide (atrial na triuretic peptide, ANP) and β-myosin heavy chain (β-myosin heavy chain , β-MHC) polyclonal antibody, horseradish enzyme-labeled rabbit anti-sheep IgG, Wuhan Boster Biotechnology Co., Ltd.; HP Sonos5500 color ultrasonic diagnostic instrument, Hewlett-Packard Company of the United States; rat sphygmomanometer, Shanghai Yuyan Scientific Instrument Co., Ltd.; Fluorescence ...

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Abstract

The invention discloses application of gynostemma pentaphylla general flavone in preparation of medicines for preventing and treating cardiac hypertrophy or diseases caused by cardiac hypertrophy. A spontaneously hypertensive rat cardiac hypertrophy model is utilized, isoprenaline which is a beta receptor stimulant induces mice cardiac muscles, and a mice cardiac hypertrophy model is copied; and rat cardiac hypertrophy is induced by coarctation of abdominal aorta, a rat cardiac hypertrophy model is copied, different dosages of a traditional Chinese medicine active ingredient which is the gynostemma pentaphylla general flavone is used, through research of pharmacodynamic integral animal experiment, a positive treatment effect is achieved, and a foundation is provided for screening of new medicines.

Description

[0001] (1) Technical field [0002] The invention relates to a new application of gynostemma total flavones, in particular to an application of gynostemma total flavonoids in the preparation of medicines for preventing and treating myocardial hypertrophy. [0003] (2) Background technology [0004] Gynostemma pentaphyllum, also known as Aescin, is mainly a plant of the genus Gynostemma cucurbitaceae. It has the effects of cold in nature, sweet in taste, calming the nerves, beneficial to qi, and lowering blood pressure. Therefore, among the folks, it is regarded as a magical "herb for longevity and longevity", and it is at the top of the precious Chinese medicinal materials. The results of basic pharmacodynamic studies show that it has obvious pharmacological effects such as anti-inflammatory, anti-immune, analgesic, sedative, antitussive, hypolipidemic, antiarrhythmic, and leukocytosis. [0005] The total flavonoids of Gynostemma pentaphyllum has a protective effect on hypoxia...

Claims

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Application Information

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IPC IPC(8): A61K36/424A61P9/00A61P9/12A61P9/10A61P7/06A61P29/00A61P11/00A61P5/16
CPCA61K36/424A61K2236/333A61K2236/51A61K2236/55
Inventor 李乐叶雨婷尚好
Owner ZHEJIANG UNIV OF TECH
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