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Application of black phosphorus nanoparticles as bio-safe two-photon contrast agent

A nanoparticle, biosafety technology, applied in the fields of biology and optics, can solve problems such as cytotoxicity, and achieve the effect of low excitation ability, easy preparation, and huge application potential

Inactive Publication Date: 2018-05-01
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing commonly used two-photon dyes, such as rhodamine, have certain cytotoxicity

Method used

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  • Application of black phosphorus nanoparticles as bio-safe two-photon contrast agent
  • Application of black phosphorus nanoparticles as bio-safe two-photon contrast agent
  • Application of black phosphorus nanoparticles as bio-safe two-photon contrast agent

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Two-photon cell imaging of black phosphorus nanoparticles, a novel biosafe two-photon contrast agent

[0024] The ground black phosphorus crystals are prepared into nanoscale particles by liquid phase exfoliation, and the prepared black phosphorus nanoparticles are suspended in a suitable solvent. The simplest and non-toxic solvent can choose ultrapure water,

[0025] Adjust the black phosphorus nanoparticle suspension concentration. The adjusted concentration of black phosphorus nanoparticle suspension was incubated with cells for an appropriate amount of time, and two-photon imaging was performed on living cells after the cells swallowed an appropriate amount of black phosphorus.

Embodiment 2

[0027] Two-photon animal imaging of biosafe two-photon contrast agent black phosphorus nanoparticles

[0028] Adjust the black phosphorus nanoparticle suspension to an appropriate concentration. The prepared mice were anesthetized, and after the mice lost consciousness, the black phosphorus nanoparticle suspension with adjusted concentration was intravenously injected into the mice. On the premise of not harming the mice, the injected mice were fixed on a two-photon microscope stage, and the ear blood vessels were imaged.

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Abstract

The invention discloses an application of black phosphorus nanoparticles as a bio-safe two-photon contrast agent, and belongs to the fields of biology and photology. The invention provides the application of the black phosphorus nanoparticles as the bio-safe two-photon contrast agent. The particle size range of the black phosphorus nanoparticles is 10-100nm; and the black phosphorus nanoparticlesare applicable to cells or organisms.

Description

technical field [0001] The invention belongs to the fields of biology and optics, and relates to a near-infrared two-photon contrast agent and a novel two-dimensional material and applications thereof, in particular to a two-photon contrast agent that can be used for biological imaging; the near-infrared two-photon contrast agent has low biological toxicity, Easy to degrade, high efficiency of single-photon and two-photon fluorescence. Background technique [0002] Due to the birth of femtosecond pulsed lasers, microscopy techniques that are more advantageous than confocal microscopy techniques, such as two-photon microscopy, emerged as the times require. The excitation light used in the two-photon technology is in the near-infrared band, and the light wavelength in the near-infrared band carries less energy, so it does little damage to biological tissues; and the near-infrared light (wavelength range of 780-3000nm) has strong penetrating power , so the near-infrared light ...

Claims

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Application Information

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IPC IPC(8): A61K49/00G01N21/84
CPCA61K49/0013G01N21/84
Inventor 王秀红孟小童
Owner BEIJING UNIV OF TECH
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