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Stabilizer for α-hydroxybutyrate dehydrogenase assay kit and preparation method thereof

A technology of hydroxybutyrate dehydrogenase and stabilizer, which is applied in the measurement of color/spectral characteristics, chemical analysis by catalysis, instruments, etc. It can solve the problems of low accuracy and poor stability, and achieve low cost , to ensure accuracy, to prolong the effect of storage time

Active Publication Date: 2021-05-28
ZHONGSHAN CHUANGYI BIOCHEM ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current kits on the market have problems of poor stability and low accuracy

Method used

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  • Stabilizer for α-hydroxybutyrate dehydrogenase assay kit and preparation method thereof
  • Stabilizer for α-hydroxybutyrate dehydrogenase assay kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A stabilizer for α-hydroxybutyrate dehydrogenase kit, comprising the following components: 2.00g / L edetate disodium, 10.00g / L sucrose, 10.00g / L Triton X-100, 30.00g / LCCD, 10.00g / L glycerin and 2.00g / L liquid biological preservative Proclin300, the balance is water.

[0024] Weigh each component according to any of the above, add disodium edetate, glycerin, CCD and sucrose in sequence in the dissolution container, add an appropriate amount of water and stir to dissolve completely; then add Triton X-100 to fully emulsify After the emulsification is complete, add the liquid biological preservative Proclin300 and a sufficient amount of water, stir to dissolve it completely, filter, and subpackage to prepare a stabilizer for the α-hydroxybutyrate dehydrogenase kit.

[0025] Example 1 was applied to the α-hydroxybutyrate dehydrogenase assay kit, and evaluated from two aspects of long-term stability, validity period and usage amount.

[0026] (1) Stability, accuracy and preci...

Embodiment 2

[0051] A stabilizer for α-hydroxybutyrate dehydrogenase kit, comprising the following components: 2.50g / L edetate disodium, 15.00g / L sucrose, 12.00g / L Triton X-100, 40.00g / LCCD, 12.00g / L glycerin and 2.00g / L liquid biological preservative Proclin300, the balance is water.

[0052] Weigh each component according to any of the above, add disodium edetate, glycerin, CCD and sucrose in sequence in the dissolution container, add an appropriate amount of water and stir to dissolve completely; then add Triton X-100 to fully emulsify After the emulsification is complete, add the liquid biological preservative Proclin300 and a sufficient amount of water, stir to dissolve it completely, filter, and subpackage to prepare a stabilizer for the α-hydroxybutyrate dehydrogenase kit.

Embodiment 3

[0054] A stabilizer for α-hydroxybutyrate dehydrogenase kit, comprising the following components: 3.00g / L edetate disodium, 20.00g / L sucrose, 10.00g / L Triton X-100, 35.00g / LCCD, 10.00g / L glycerin and 2.00g / L liquid biological preservative Proclin300, the balance is water.

[0055] Weigh each component according to any of the above, add disodium edetate, glycerin, CCD and sucrose in sequence in the dissolution container, add an appropriate amount of water and stir to dissolve completely; then add Triton X-100 to fully emulsify After the emulsification is complete, add the liquid biological preservative Proclin300 and a sufficient amount of water, stir to dissolve it completely, filter, and subpackage to prepare a stabilizer for the α-hydroxybutyrate dehydrogenase kit.

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Abstract

The present invention disclosed a stabilizer and preparation method for α -hydroxyl acid dehydrogenase testing kit, which belongs to the field of in vitro diagnostic.The stabilizer includes the following percentage components: 0.1 ~ 0.5 % ethyleine tetharolytic acid sodium, 1 to 3 % sucrose, 1 to 2 % Triton X‑100, 3 to 5 % CCD, 1 to 2 % glycerin, 0.1 ~ 0.2 % liquid biochemical PROCLIN300, the remaining amount of water.The stabilizer of the present invention is used in the α -hydroxyl acid dehydrogenase testing kit, which ensures the accuracy of the test results of the kit, and makes the alpha -hydroxyl acid dehydrogenase test box more stable, which can effectively extend the reagent reagentThe preservation time of the box.In addition, the cost of the stabilizer is low, the preparation is simple, and it is worth further promotion.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, and in particular relates to a stabilizer used in an α-hydroxybutyrate dehydrogenase assay kit and a preparation method thereof. Background technique [0002] α-Hydroxybutyrate dehydrogenase (α-HBDH) is an enzyme in the myocardial enzyme spectrum, which is ubiquitous in mammals and mainly distributed in tissues or organs such as myocardial red blood cells, white blood cells and kidneys. α-Hydroxybutyrate dehydrogenase (α-HBDH) is not an independent specific enzyme, but a general term for lactate dehydrogenase isozymes LDH1 and LDH2 containing H subunits. Determination of α-hydroxybutyrate dehydrogenase actually reflects the activity of lactate dehydrogenase isoenzymes LDH1 and LDH2, which is of great significance for the diagnosis of myocardial disease and liver disease. Increased α-HBDH activity is common in acute myocardial infarction, skeletal muscle injury, acute hepatitis, leukemia and mal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N31/10G01N35/00
CPCG01N21/31G01N31/10G01N35/00
Inventor 黄小全崔海林曾晓君黄清媚
Owner ZHONGSHAN CHUANGYI BIOCHEM ENG
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