Cape jasmine lycoypene beta-cyclase b2 gene, protein encoded by same, gene optimized by same and application thereof

A technology of lycopene and gene encoding, which is applied in the field of gardenia lycopene beta-cyclase b2 gene and the protein encoded by it

Inactive Publication Date: 2018-05-08
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports on the primers and their nucleotide and protein sequences for amplifying the full-length gene of gardenia lycopene β-cyclase b2 at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cape jasmine lycoypene beta-cyclase b2 gene, protein encoded by same, gene optimized by same and application thereof
  • Cape jasmine lycoypene beta-cyclase b2 gene, protein encoded by same, gene optimized by same and application thereof
  • Cape jasmine lycoypene beta-cyclase b2 gene, protein encoded by same, gene optimized by same and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Materials and methods

[0031] Gardenia fruit was divided into three development stages. The first one is picked at three months of development, in the green fruit stage, the seeds are immature, the seed coat is very soft, the peel is green, and the flesh is light yellow; the second is picked at five months of development, in the yellow fruit stage, the seeds Slightly hard, the peel is orange-yellow, and the pulp is orange; the third is picked when it is seven months old, and it is in the red fruit stage. The peel and pulp are red, and the seeds are hard. The persistent calyx and fruit stalk of the three periods are green without reddening. Take the fruits of three stages, peel off the pulp and seeds, and take the leaves and stems of the third stage to detect the expression of genes in different tissues and different developmental stages of fruits.

[0032] RACE kit (Clontech SMARTerTM RACE cDNA Amplification Kit) and pMD18-T vector kit (No. D101A) were purchased f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biologics and relates to a cape jasmine lycoypene beta-cyclase b2 gene, a protein encoded by same, a gene optimized by same and application thereof. Thecape jasmine lycoypene beta-cyclase b2 gene can be applied to preparation of DNA (Deoxyribonucleic Acid) probes, can be transferred into bioreactors of plants and microorganisms, is capable of generating corresponding carotenoid metabolic components in modes such as expression or expression inhibition, and has great significances for regulation and control on saffron total glycoside biosyntheticpathways, or production of saffron total glycosides with bioreactors. The amino acid sequences and prokaryotic expression bacteria of the protein can be adopted to produce cape jasmine lycoypene beta-cyclase b2 or prepare antibodies.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to a gardenia lycopene β-cyclase b2 gene, its encoded protein, an optimized gene and applications thereof. Background technique [0002] The dried and mature fruits of Gardenia jasminoides Eills. contain crocin total glycosides, which have important pharmacological activities, including: antidepressant, prevention of retinal degeneration, treatment of neurodegenerative diseases, treatment of hypertension and hyperlipidemia, treatment of diabetes Wait. "Gardenia yellow" pigment with saffron glycosides as the main component is widely used in the production of food, beverage, medicine, cosmetics, etc. It is a popular natural food additive in the world, and the market demand is huge. [0003] It has been reported that the total glycosides of saffron are only synthesized in the fruit of Gardenia jasminoides, not in the leaves and stems, and the synthesis amount of the pulp is the highest; Fr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/61C12N9/90C12N15/11
CPCC12N9/90C12Y505/01019
Inventor 王晓云王鹏良徐旭
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap