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Application of trigonelline in preparation of medicine or food for treating or preventing hypoxic injury

A technology of trigonelline and medicine, which is applied in the application field of trigonelline, medicine or food, and can solve problems such as limitations, obvious side effects, and difficulty in wide-scale promotion and use

Active Publication Date: 2019-09-20
杭州方回春堂健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are mainly chemical drugs and Chinese patent medicines for anti-hypoxic drugs. Chemical drugs such as acetazolamide, dexamethasone, etc., although they have good anti-hypoxic effects, are limited due to their obvious side effects; Chinese patent medicines include ginseng, rhodiola rosea, snow lotus, etc. Although they can effectively improve the body's hypoxia tolerance, they are difficult to be widely used due to limited resources and high prices.
However, there is no report on anti-hypoxic activity research

Method used

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  • Application of trigonelline in preparation of medicine or food for treating or preventing hypoxic injury
  • Application of trigonelline in preparation of medicine or food for treating or preventing hypoxic injury
  • Application of trigonelline in preparation of medicine or food for treating or preventing hypoxic injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Hypoxic nerve cell damage protection test.

[0028] experiment method:

[0029] 1) Inoculate 100 μL (about 5×10 3 a) dPC12 cells in a 96-well plate at 37°C, 5% CO 2 The cells were cultured in a normal incubator for 24h to make the cells adhere and grow normally.

[0030] 2) Carefully aspirate the medium, add the sample solution, and set up trigonelline low-dose group (0.1 μg / mL), middle-dose group (1.0 μg / mL), high-dose group (10.0 μg / mL), and hypoxia control and 5 duplicate wells of normal control group at 37℃, 5%CO 2 After incubation in the normal incubator for 3 h, except the normal control group, the culture plates of the other groups were transferred to 37 °C, 0.5% O 2 +5%CO 2 +94.5%N 2 cultured in a three-gas incubator for 36 h.

[0031] 3) Carefully add 10 μL of CCK-8 solution to each well in the ultra-clean workbench (be careful to avoid light), and mix well. Incubate in the incubator for 3h, and measure the absorbance at 450nm wavelength with a micropla...

Embodiment 2

[0039] Hypoxic cardiomyocyte injury protection test.

[0040] experiment method:

[0041] 1) Inoculate 100 μL (about 1×10 4 A) H9C2 cells in 96-well plates at 37°C, 5% CO 2 The cells were cultured in a normal incubator for 24h to make the cells adhere and grow normally.

[0042] 2) Carefully aspirate the medium, add the sample solution, and set up trigonelline low-dose group (0.1 μg / mL), middle-dose group (1.0 μg / mL), high-dose group (10.0 μg / mL), and hypoxia control and 5 duplicate wells of normal control group at 37℃, 5%CO 2 After incubation in the normal incubator for 3 h, except the normal control group, the culture plates of the other groups were transferred to 37 °C, 0.5% O 2 +5%CO 2 +94.5%N 2 cultured in a three-gas incubator for 24 h.

[0043] 3) Carefully add 10 μL of CCK-8 solution to each well in the ultra-clean workbench (be careful to avoid light), and mix well. Incubate in the incubator for 3h, and measure the absorbance at 450nm wavelength with a micropl...

Embodiment 3

[0049] Normobaric hypoxia tolerance mouse test.

[0050] 60 healthy Kunming male mice, weighing 18-22 g, were provided by the Animal Center of Zhejiang University and acclimated to the environment for 7 days before the experiment. Randomly divided into 4 groups according to body weight: blank control group (distilled water), positive control group (acetazolamide: 200mg / kg.bw / d), trigonelline low-dose group (100mg / kg.bw / d) and fenugreek Alkali high dose group (300mg / kg.bw / d), 15 in each group. The samples were fully dispersed and dissolved in distilled water, and after oral administration for 7 days, the normal pressure hypoxia tolerance mouse test was carried out. During the experiment, the mice were free to drink water and eat, and observe the food intake, physiological and mental status of the mice.

[0051] According to the test method for improving the function of hypoxia tolerance in the functional evaluation test method of "Technical Specifications for Functional Food ...

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Abstract

The invention discloses new application of gynesine, namely application of the gynesine in preparing a medicine or food for treating or preventing hypoxic damage, and the medicine or the food preparedfrom the gynesine and used for treating or preventing hypoxic damage. The research finds that the gynesine has a function of treating or preventing hypoxic damage, and can be used for preparing the medicine or the food for treating or preventing hypoxic damage; a cell experiment and an animal experiment prove that the gynesine can be beneficial to protecting hypoxic nerve cell damage, reducing myocardial apoptosis caused by hypoxia, prolonging the survival time of rats in a normal pressure anoxia tolerance test, and increasing the survival rate of the rats in an acute low pressure hypoxia test.

Description

technical field [0001] The invention relates to the technical field of medicine and food, in particular to the application of trigonelline in the preparation of medicine or food for treating or preventing hypoxic injury. Background technique [0002] Hypoxia is a pathological state in which the oxygen required for the body's life activities cannot be adequately supplied. With the change of people's way of life and the acceleration of life rhythm, hypoxia has gradually become a common pathological phenomenon, which seriously affects human health. High altitude hypobaric and hypoxic environment is one of the most common conditions for humans to experience hypoxia, which often causes acute altitude sickness such as chest tightness, vomiting, thirst, dizziness, difficulty breathing, strong fatigue, and purple face in people who rush to the plateau. It can even cause high altitude sickness, such as high altitude pulmonary edema, high altitude cerebral edema, etc., which are life...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/455A61P39/00A61P9/00A61P9/10A61P25/00A23L33/10
Inventor 楚秉泉何乐蔡海莺
Owner 杭州方回春堂健康科技有限公司
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