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Application of gynesine in preparing medicine or food for treating or preventing hypoxic damage

A technology of trigonelline and medicine, which is applied in the application field of trigonelline, medicine or food, and can solve problems such as obvious side effects, no anti-hypoxic activity, and limited resources

Active Publication Date: 2018-05-11
杭州方回春堂健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are mainly chemical drugs and Chinese patent medicines for anti-hypoxic drugs. Chemical drugs such as acetazolamide, dexamethasone, etc., although they have good anti-hypoxic effects, are limited due to their obvious side effects; Chinese patent medicines include ginseng, rhodiola rosea, snow lotus, etc. Although they can effectively improve the body's hypoxia tolerance, they are difficult to be widely used due to limited resources and high prices.
However, there is no report on anti-hypoxic activity research

Method used

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  • Application of gynesine in preparing medicine or food for treating or preventing hypoxic damage
  • Application of gynesine in preparing medicine or food for treating or preventing hypoxic damage
  • Application of gynesine in preparing medicine or food for treating or preventing hypoxic damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Hypoxic nerve cell damage protection test.

[0028] experiment method:

[0029] 1) Inoculate 100 μL (about 5×10 3 each) dPC12 cells in 96-well plate, at 37°C, 5% CO 2 Conditioned normal incubator for 24h to make the cells adhere to the wall and grow normally.

[0030] 2) Carefully suck out the medium, add the sample solution, and set up a low-dose trigonelline group (0.1 μg / mL), a medium-dose group (1.0 μg / mL), a high-dose group (10.0 μg / mL), and a hypoxic control group and the normal control group, each with 5 replicate wells, at 37°C, 5% CO 2 After incubating for 3 hours in a normal incubator with normal conditions, except for the normal control group, the culture plates of the other groups were transferred to 37°C, 0.5% O 2 +5%CO 2 +94.5%N 2 Cultivate for 36 hours under the conditions of the three-gas incubator.

[0031] 3) Carefully add 10 μL of CCK-8 solution to each well in an ultra-clean workbench (protect from light), and mix well. Incubate in an incubat...

Embodiment 2

[0039] Hypoxic cardiomyocyte injury protection assay.

[0040] experiment method:

[0041] 1) Inoculate 100 μL (about 1×10 4 each) H9C2 cells in a 96-well plate at 37°C, 5% CO 2 Conditioned normal incubator for 24h to make the cells adhere to the wall and grow normally.

[0042] 2) Carefully suck out the medium, add the sample solution, and set up a low-dose trigonelline group (0.1 μg / mL), a medium-dose group (1.0 μg / mL), a high-dose group (10.0 μg / mL), and a hypoxic control group and the normal control group, each with 5 replicate wells, at 37°C, 5% CO 2 After incubating for 3 hours in a normal incubator with normal conditions, except for the normal control group, the culture plates of the other groups were transferred to 37°C, 0.5% O 2 +5%CO 2 +94.5%N 2 Cultivate for 24 hours in a trigas incubator.

[0043] 3) Carefully add 10 μL of CCK-8 solution to each well in an ultra-clean workbench (protect from light), and mix well. Incubate in an incubator for 3 h, and measur...

Embodiment 3

[0049] Atmospheric tolerance to hypoxia mouse test.

[0050] Take 60 healthy Kunming male mice, weighing 18-22g, provided by the Animal Center of Zhejiang University, and adapt to the environment for 7 days before the experiment. Randomly divided into 4 groups according to body weight: blank control group (distilled water), positive control group (acetazolamide: 200mg / kg.bw / d), low-dose trigonelline group (100mg / kg.bw / d) and trigonelline Alkaline high dose group (300mg / kg.bw / d), 15 rats in each group. Each sample was fully dispersed and dissolved in distilled water, and after oral gavage administration for 7 days, the normal-pressure hypoxia-resistant mouse test was carried out. During the experiment, the mice were allowed to drink water and eat freely, and the mice's food intake, physiological and mental conditions were observed.

[0051] According to the test method for improving the hypoxia tolerance function in the functional evaluation test method of "Functional Food Te...

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Abstract

The invention discloses new application of gynesine, namely application of the gynesine in preparing a medicine or food for treating or preventing hypoxic damage, and the medicine or the food preparedfrom the gynesine and used for treating or preventing hypoxic damage. The research finds that the gynesine has a function of treating or preventing hypoxic damage, and can be used for preparing the medicine or the food for treating or preventing hypoxic damage; a cell experiment and an animal experiment prove that the gynesine can be beneficial to protecting hypoxic nerve cell damage, reducing myocardial apoptosis caused by hypoxia, prolonging the survival time of rats in a normal pressure anoxia tolerance test, and increasing the survival rate of the rats in an acute low pressure hypoxia test.

Description

technical field [0001] The invention relates to the technical field of medicine and food, in particular to the application of trigonelline in the preparation of medicine or food for treating or preventing hypoxic injury. Background technique [0002] Hypoxia refers to a pathological state when the oxygen required by the body's vital activities cannot be supplied adequately. With the change of people's lifestyle and the quickening pace of life, hypoxia has gradually become a common pathological phenomenon, seriously affecting human health. Plateau low-pressure hypoxic environment is one of the most common conditions for human beings to experience hypoxia. People who rush to the plateau often experience acute altitude sickness such as chest tightness, vomiting, thirst, dizziness, dyspnea, strong fatigue, and purple face. Sometimes even cause the occurrence of high altitude sickness, such as high altitude pulmonary edema, high altitude cerebral edema, etc., threatening life. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/455A61P39/00A61P9/00A61P9/10A61P25/00A23L33/10
Inventor 楚秉泉何乐蔡海莺
Owner 杭州方回春堂健康科技有限公司
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