Method for preparing ganoderma lucidum mycelium fermentation liquid, yeast and application of liquid
A technology of Ganoderma lucidum mycelium and fermentation liquid, which is applied in the field of fermentation, and can solve the problems of time-consuming and labor-intensive artificial cultivation of fruit bodies, difficulty in ensuring product quality, and high cost, and achieve the effects of short cultivation period, stable product quality and easy breakage
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Embodiment 1
[0045] A method for preparing ganoderma lucidum mycelia fermentation liquid, said method comprising the following steps:
[0046] (1), preparation of culture medium
[0047] Plate medium (1000mL): 30g malt extract, 3g soybean peptone, 30g agar, dilute to 1L with water, pH5.6, sterilize at 121°C for 15min.
[0048] Malt extract powder liquid fermentation medium (1000mL): malt extract powder 20g, glucose 20g, peptone 1g, yeast extract 1g, dilute to 1L with water, sterilize at 115°C for 20min.
[0049] CYM medium (1000mL): glucose 20g, peptone 2g, yeast powder 2g, MgSO 4 ﹒ 7H2 O 0.5g, K 2 HPO 4 1g, KH 2 PO 4 0.46g, dilute to 1L with water.
[0050] (2) liquid fermentation culture method of ganoderma lucidum strain
[0051] Each Ganoderma lucidum strain (American Ganoderma lucidum, Chinese Ganoderma lucidum, Antler Ganoderma lucidum, and Black lucidum) was cultured in inclined test tubes, and one piece of 1cm 2 The mycelium pieces were inoculated into plate culture mediu...
Embodiment 2
[0053] Get the fermented liquid that contains mycelium, detect the ability of described fermented liquid to scavenge DPPH free radical, concrete detection method is as follows:
[0054] Determination of the ability to scavenge DPPH free radicals: Take 1ml of the solution to be tested and add 1ml of DPPH with a concentration of 0.2mmol / L. After standing at room temperature for 30 minutes, measure the change in absorbance at a wavelength of 517nm. Substitute the absorbance into the following formula to calculate the DPPH scavenging rate.
[0055] DPPH clearance rate (%) = [1-(A i -A j ) / A 0 ]×100%;
[0056] In the formula: A i Absorbance of 1ml of DPPH+1ml sample; A j Absorbance of 1ml solvent + 1ml sample; A 0 It is the absorbance of 1ml DPPH+1ml solvent.
[0057] The specific test results are shown in Table 1. The mycelium fermentation broth (American Ganoderma lucidum, Chinese red paper, Antler Ganoderma lucidum, and Black lucidum) obtained from malt extract powder liqu...
Embodiment 3
[0062] Get the fermented liquid that contains mycelium, detect described fermented liquid to remove the free ability of superoxide anion, concrete detection method is as follows:
[0063] (1) Take 5.7ml of 50mmol / L Tris-HCl buffer solution with pH 8.2 in a 10ml EP tube, add 0.2ml of sample solution into it, mix well and incubate at 25°C for 10min;
[0064] (2) Add 0.1ml of pyrogallol solution of 10mmol / L preheated at 25°C, the total volume is 6ml, shake it up quickly and record the increase value of its absorbance at 320nm wavelength within 1min with a UV-visible spectrophotometer (A s ).
[0065] (3) Calculate the increase in absorbance per minute in the linear range. Take another reagent as above, replace the sample liquid with an equal volume of water, and measure the increase in absorbance (A 0 ).
[0066] See Table 2 for specific test results. According to the test results described in Table 2, it can be seen that the efficiency of scavenging superoxide anion free ra...
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