Method for screening rhizosphere biocontrol bacterium microorganisms of panax notoginseng
A technology of microorganisms and bio-control bacteria, which is applied in the measurement/inspection of microorganisms, biochemical equipment and methods, and the separation of microorganisms, etc., and can solve the problems of not being able to play a good role in prevention and control
Pending Publication Date: 2018-05-15
YUNNAN AGRICULTURAL UNIVERSITY
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[0004] The purpose of the present invention is to provide a method for screening from the soil of Panax notoginseng, which can not only utilize the secretion of Panax notoginseng root system, but also to solve the shortcomings that the beneficial microorganisms screened out by the in vitro inhibition zone method can not play a good control role in the rhizosphere of Panax notoginseng. The saponin is carried out as nutrition and can effectively antagonize the microbial method of root rot fungus
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[0024] (1) Extraction of crude saponins of metabolites from the roots of Panax notoginseng. Choose healthy three-year-old Panax notoginseng roots, wash the surface with tap water, and cut into 1-2cm slices, air-dried at room temperature, and then grind into powder with a mill and pass through a 100-mesh screen. Weigh 40g of Panax notoginseng powder into a 50mL Erlenmeyer flask (500ml), and ultrasonically extract with 200mL 80% methanol at 30°C for 40min. After extraction, centrifuge (12000rpm, 4°C, 5min), and collect the supernatant. The supernatant was concentrated under reduced pressure on a rotary evaporator at 40°C to remove methanol, then freeze-dried into a powder, and stored in a refrigerator at -20°C for later use.
[0025] (2) Use crude saponin to make selective medium. Weigh NaNO separately 3 3g, KH 2 PO 4 1g, KCl 0.5g, MgSO4·7H 2 O 0.5g, FeSO 4 ·7H 2 Dissolve 0.01g of O, 10g of crude saponin, and 15g of agar in a 1L glass beaker, mix well, and dilute to 1L, adjust ...
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The invention relates to a method for screening multifunctional beneficial microorganisms, belonging to the field of plant protection. The method aims at the shortcomings that beneficial microorganisms screened out by using an in-vitro bacteriostatic circle method can not play prevention and control roles at the rhizosphere of panax notoginseng; the invention provides a method for screening microorganisms, which can not only use saponins secreted by the root system of the panax notoginseng as nutrition for growing, but also effectively antagonize root rot bacteria, from the soil of the panax notoginseng. The method comprises the steps of firstly, using the root system of the panax notoginseng to extract metabolite crude saponins, then making a selective medium by using the extracted crudesaponins, using the selective culture medium to screen the microorganisms which use the saponins as the nutrition for growing, and finally screening the microorganisms having an antagonistic ability for the root rot bacteria by using a confrontation culture method. The microbial strains screened out by using the method not only can be well colonized at the rhizosphere of the panax notoginseng, butalso effectively antagonize the root rot bacteria, thus finally realizing the ecological prevention and control of root rot caused during the planting of the panax notoginseng.
Description
Technical field [0001] The invention relates to a method for screening multifunctional beneficial microorganisms in the field of plant protection, in particular to a method for screening microorganisms that can not only colonize the rhizosphere but also antagonize the root rot of notoginseng. Background technique [0002] Notoginseng (Panax notoginseng (Burk.) F.H. Chen) belongs to the genus Araliaceae and is a rare Chinese medicinal material unique to our country. It has the functions of dispelling blood stasis, stopping bleeding, reducing swelling and relieving pain, and has significant effects on the treatment of cardiovascular and cerebrovascular diseases. Yunnan is the main producing area and origin of Panax notoginseng. However, there is a serious problem of continuous cropping obstacles in the growth of Panax notoginseng, and the continuous cropping obstacles have seriously affected the yield and quality of Panax notoginseng due to large-scale single planting year after ye...
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Login to View More IPC IPC(8): C12N1/02C12Q1/04C12Q1/18
CPCC12N1/02C12Q1/045C12Q1/18
Inventor 杨敏罗丽芬朱书生袁也黄惠川刘屹湘何霞红朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY