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Method for constructing artificial seeds with pleione protocorm as propagule

An artificial seed, protocorm-like technology, applied in gardening methods, botanical equipment and methods, gardening tools/equipment, etc., to facilitate storage, improve air permeability, and solve the effects of increasing depletion

Active Publication Date: 2018-05-18
普洱昆弘生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report about the artificial seeds of Suanlan orchid at home and abroad

Method used

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  • Method for constructing artificial seeds with pleione protocorm as propagule
  • Method for constructing artificial seeds with pleione protocorm as propagule
  • Method for constructing artificial seeds with pleione protocorm as propagule

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Embodiment Construction

[0033] The technical solution of the present invention will be further described below in conjunction with the embodiments.

[0034] The preparation method of solitary orchid artificial seed of the present invention, comprises the following steps:

[0035] (1) Induction of protocorm-like:

[0036] Inoculate the aseptic seedlings of Allium chinensis in protocorm-like induction medium to induce the formation of protocorm-like at the base;

[0037] (2) Proliferation of protocorms:

[0038] Transferring the induced protocorm-like to the proliferation medium, carrying out the proliferation culture for 30-35 days, and then transferring to the liquid medium to carry out the proliferation for 15-30 days;

[0039] (3) Production of artificial seeds:

[0040] Preparation of artificial endosperm;

[0041] Preparation of artificial seed coat;

[0042] The artificial endosperm and artificial seed coat were autoclaved at 121°C for 30 minutes respectively, and cooled for later use;

[...

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Abstract

The invention discloses a method for constructing artificial seeds with pleione protocorm as a material. The method comprises the steps: induction and multiplication of the pleione protocorm and production and sowing of the artificial seeds. The method is characterized in that a plant tissue culture technology is combined with an artificial seed technology, and protocorm-like bodies generated by inducing base parts of pleione seedlings are utilized and multiplied to serve as propagules of the artificial seeds, so that large-scale production can be realized. The method has the advantages of noinfluence from the season, short breeding cycle, low cost and convenience in storage; the problem of increasing depletion of pleione resources is effectively solved and a technical support is providedfor industrial development of pleione.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for making artificial seeds of orchid orchids and a sowing method thereof. Background technique [0002] The dried pseudobulb of the orchid plant Pleione bulbocodioides (Franch.) Rolfe is one of the main sources of the traditional Chinese medicine Arrowhead. The 2015 edition of "Chinese Pharmacopoeia" records that the main sources of Arrowhead are the orchid plants Rhododendron orchid (Cremastra appendiculata (D.Don) Makino), only garlic orchid (Pleione bulbocodioides (Franch.) Rolfe) or Yunnan single garlic orchid (Pleione yunnanensis ( Dried pseudobulbs of Rolfe) Rolfe). The former is commonly called "Mao Cigu", and the latter two are commonly called "Hockey Ball", both of which are used as Shan Cigu. It is sweet in taste, slightly pungent, cool in nature, has the effects of clearing away heat and detoxification, reducing swelling and dispelling stagnati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/006
Inventor 丁志山俞洁林明杨云红高永富
Owner 普洱昆弘生物科技有限公司
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