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A kind of compound lactic acid bacteria agent and its preparation method and application

A technology for compounding lactic acid bacteria and inoculants, applied in the biological field, can solve problems such as less research on microorganisms, and achieve the effects of shortening fruiting time, increasing yield, and simple and easy method.

Active Publication Date: 2021-01-05
金华银河生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are different opinions on the effect factors of covering soil. At present, the discussion is mainly from three aspects: the physical action, chemical action and microbial action of covering soil. In contrast, there are few studies on microorganisms.

Method used

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  • A kind of compound lactic acid bacteria agent and its preparation method and application
  • A kind of compound lactic acid bacteria agent and its preparation method and application
  • A kind of compound lactic acid bacteria agent and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0071] The preparation of embodiment 1 compound lactic acid bacteria inoculum

[0072] Step 1, inoculate the frozen preservation strains of Lactobacillus plantarum P-8, Lactobacillus acidophilus KT-La9 and Lactobacillus plantarum KT-Lp9 in MRS liquid medium respectively, and culture them at 37±1°C for 26 hours respectively , after being activated in this way for three generations, centrifuge and wash 3 times with sterile normal saline respectively to obtain the thallus, after adding 8wt% sterilized skim emulsion on each thalline sediment respectively, the three kinds of seed liquid thalline concentrations are adjusted to 1×10 8 ~1×10 9 CFU / mL, that is, the preparation of the seed solution is completed;

[0073] Step 2, the seed liquid of Lactobacillus plantarum P-8, Lactobacillus acidophilus KT-La9 and Lactobacillus plantarum KT-Lp9 obtained in step 1 is mixed according to the ratio of 3:0.5:1, wherein the amount of each thalline In terms of the number of viable bacteria ...

Embodiment 2

[0074] The preparation of embodiment 2 compound lactic acid bacteria inoculum

[0075] Step 1, inoculate the frozen preservation strains of Lactobacillus plantarum P-8, Lactobacillus acidophilus KT-La9 and Lactobacillus plantarum KT-Lp9 in MRS liquid medium respectively, and culture them at 37±1°C for 32 hours respectively After being activated for three generations in this way, wash them twice with sterile normal saline to obtain the thallus, add 10wt% sterilized skim emulsion to each thalline sediment, and adjust the concentration of the three kinds of seed liquid thallines to 1×10 8 ~1×10 9 CFU / mL, that is, the preparation of the seed solution is completed;

[0076] Step 2, mix the seed liquid of Lactobacillus plantarum P-8, Lactobacillus acidophilus KT-La9 and Lactobacillus plantarum KT-Lp9 obtained in step 1 according to the ratio of 2:1:1, wherein the amount of each thalline In terms of the number of viable bacteria therein, the composite bacteria seed solution was ...

Embodiment 3

[0077] The preparation of embodiment 3 compound lactic acid bacteria inoculum

[0078] Step 1, inoculate the frozen preservation strains of Lactobacillus plantarum P-8, Lactobacillus acidophilus KT-La9 and Lactobacillus plantarum KT-Lp9 in MRS liquid medium respectively, and culture them at 37±1°C for 20 hours respectively After being activated for three generations in this way, wash with sterile normal saline centrifuge 4 times respectively to obtain the thallus, after adding 12wt% sterilized skim emulsion respectively on each thalline sediment, adjust the thalline concentration of three kinds of seed liquids to 1×10 8 ~1×10 9 CFU / mL, that is, the preparation of the seed solution is completed;

[0079] Step 2, the seed liquid of Lactobacillus plantarum P-8, Lactobacillus acidophilus KT-La9 and Lactobacillus plantarum KT-Lp9 obtained in step 1 is mixed according to the ratio of 4:1:1, wherein the amount of each thalline Based on the number of live bacteria, the composite ...

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Abstract

The invention discloses a compound lactobacillus microbial inoculum. The compound lactobacillus microbial inoculum comprises bacterial manure of the compound lactobacillus microbial inoculum, the lactobacillus microbial inoculum and bacterial manure are used for shorting mushroom fruiting time and improving the mushroom yield. The compound lactobacillus microbial inoculum is prepared from lactobacillus plantarum P-8, lactobacillus acidophilus KT-La9 and lactobacillus plantarum KT-Lp9. The compound lactobacillus microbial inoculum or bacterial manure is mixed into a culture medium used for growing mushrooms, even though the fruiting time of agaricus bisporus is shortened by four days, the yield is improved by 10% or more. The invention further provides a preparation method of the compound lactobacillus microbial inoculum, the method is easy, convenient and practical, and the viable count in the prepared compound lactobicillus microbial inoculum is high.

Description

technical field [0001] The application belongs to the field of biotechnology, and in particular relates to a lactic acid bacteria agent and its preparation method and application. Background technique [0002] Agaricus bisporus (Agaricus bisporus) referred to as Agaricus bisporus, also known as mushrooms, mushrooms, world mushrooms, commonly known as mushrooms, belongs to the genus Agaricus Agaricus, has a certain nutritional and medical value, is currently the world's A kind of edible fungus with the largest cultivation scale and output, its output accounts for about 40% of the world's edible fungi output, and it is also my country's largest export-earning edible fungus. With the increase of market demand, Agaricus bisporus has entered the stage of facilities and large-scale cultivation. [0003] Agaricus bisporus is a kind of grass rot fungus, which has the characteristics of not covering the soil and not producing mushrooms. There are different opinions on the factors of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01G18/00C05F11/02C05F11/08C12R1/25C12R1/23
CPCC05F11/02C05F11/08C12N1/20
Inventor 高鹏飞贺润晶陈升富
Owner 金华银河生物科技有限公司
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