Culture method of cystoathyrium chinense through sporophyte propagation

A cultivation method and fern spore technology are applied in the cultivation field of fern sporophyte propagation, which can solve the problems of no sporophyte cultivation and slow cultivation speed, and achieve long cultivation period, strong survival adaptability and high survival rate. Effect

Inactive Publication Date: 2018-06-01
MIANYANG TEACHERS COLLEGE
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Problems solved by technology

[0003] In summary, the problems in the prior art are: the existing method for cultivating artificially propagated fern ramets, the speed of cultivation is slow; group cultivation of seedlings can only cultivate the prophyllum, and no one has studied how to cultivate the sporophyte , so there is no technical method for cultivating sporophytes

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  • Culture method of cystoathyrium chinense through sporophyte propagation
  • Culture method of cystoathyrium chinense through sporophyte propagation
  • Culture method of cystoathyrium chinense through sporophyte propagation

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Embodiment 1

[0050] The cultivation method of the fern sporophyte propagation provided by the embodiments of the present invention comprises the following steps:

[0051] (1) collecting the underground stem buds of Fern glabra as explant material;

[0052] (2) Disinfection and sterilization

[0053] Soak the underground stem buds of fern glabra collected in the field for 2 minutes in a washing powder solution with a concentration of 3 to 5 g / mL, gently clean the bud scales with a soft brush, rinse with running water for 2 hours, and use 0.1 After soaking the buds in % mercury chloride solution for 2-3 minutes, rinse them with sterile water for more than 5 times, dry the surface moisture of the explants with sterilized filter paper after cleaning, peel off all the scales and hairs of the buds with tweezers to obtain the buds, and then Use 75% ethanol + mercury chloride to process it, the specific process is as follows:

[0054] 75% ethanol + 0.01% mercury chloride combined with disinfecti...

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Abstract

The invention belongs to the technical field of culture of cystoathyrium chinense and discloses a culture method of cystoathyrium chinense through sporophyte propagation. Underground stems of cystoathyrium chinense are soaked in a washing powder solution with the concentration of 3-5 g / mL and treated with 75% ethanol and 0.1% mercury bichloride solution, disinfection is convenient, and damage to buds is low. A culture medium with the pH value of 5.8-6.0 is used for primary culture on the buds under 2,000-2,100 lx, the buds are promoted to rapidly germinate into GGBs (green globular bodies), the GGBs can be used for subinoculation and propagation. Compared with components of a strong seedling culture medium and a proliferation culture medium, gibberellin is added, the proportion of all components are adjusted, so that the strong seedling effect can be maximized. The method is adopted for hardening of seedlings, and the survival rate of cystoathyrium chinense seedlings can reach 80% or above.

Description

technical field [0001] The invention belongs to the technical field of fern glabra cultivation, in particular to a cultivation method for sporophyte propagation of fern glabra. Background technique [0002] The light-leaved fern is mainly distributed under the longgreen deciduous broad-leaved mixed forest in the Erlang Mountains of western Sichuan. The rhizome is short and recumbent, the leaves are near, the base is brown, slightly enlarged, the leaves are narrowly lanceolate, the pinnae are nearly opposite, flat, sessile. The sporangia are round, the cap is oval, filmy, gray-green, the spores are round-reniform, dark brown, opaque, with dense spinous protrusions on the surface. It is an endangered species of wild plants approved by the State Council of China on August 4, 1999 as a national first-class key protected wild plant. At present, this species has become a very small population species, and a large number of seedlings need to be produced in a short time to protect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 胡进耀别鹏飞向莉余凌帆王虹马月琴
Owner MIANYANG TEACHERS COLLEGE
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