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Spirulina culture solution for culture machine

A technology of spirulina and culture solution, which is applied in the direction of microorganisms, bacteria, biochemical equipment and methods, etc., can solve the problems of not being able to satisfy the growth of spirulina, achieve the effects of fast growth rate of spirulina, prolong the cultivation period, and reduce the dosage

Inactive Publication Date: 2020-03-20
深圳市太空微藻生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most Spirulina cultures are cultivated using the improved Zarrouk medium, but only relying on this formula during the cultivation process cannot meet the needs of Spirulina in the entire growth cycle of the culture machine, and some components in the trace element solution are very important. Necessary, so it is necessary to get the maximum dissolution of various nutrients in the culture medium, and use relatively few components to maximize the nutrient components in the cultivation process of Spirulina. The cultivation cycle of Spirulina is more conducive to the commercial cultivation of Spirulina

Method used

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  • Spirulina culture solution for culture machine
  • Spirulina culture solution for culture machine
  • Spirulina culture solution for culture machine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Measure 10L of distilled water and place it in the culture machine;

[0019] (2) by FeSO 4 ·7H 2 O-0.1g, EDTA-0.4g, CaCl 2 2H 2 O-0.2g, MgSO 4 ·7H 2 O-2g, NaCl-2g, K 2 SO 4 -5g, KNO 3 -20g, K 2 HPO 4 -5g, NaHCO 3 - 100g is dissolved in water one by one, stirring and / or blowing while putting in, each time after the previous component is dissolved, then put in the next component, and so on, until all the components are dissolved ;

[0020] (3) Add 25g of Spirulina algae species to the culture medium formed in (2), and then use an air pump to agitate the culture medium, light for 10 hours a day, the light intensity is about 50μmol / (㎡·S), and the temperature is controlled at 25 degrees Celsius ;

[0021] (4) Check the pH value, when the pH value is between 10-12, press H 3 BO 3 -20g, MnCl 2 4H 2 O-15g, ZnSO 4 ·7H 2 O-2g, CuSO 4 ·5H 2 O-0.4g, Mo 7 o 24 6(NH 4 )·4H 2 O-0.1g, Co(NO 3 ) 2 ·6H 2 O-40mg order to add trace element supplement solutio...

Embodiment 2

[0024] (1) Measure 10L of distilled water and place it in the culture machine;

[0025] (2) by FeSO 4 ·7H 2 O-0.1g, EDTA-0.4g, CaCl 2 2H 2 O-0.3g, MgSO 4 ·7H 2 O-3g, NaCl-2g, K 2 SO 4 -5g, KNO 3 -25g, K 2 HPO 4 -6g, NaHCO 3 - 120g is dissolved in water one by one, stirring and / or blowing while adding, each time after the previous component is dissolved, then put in the next component, and so on, until all the components are dissolved ;

[0026] (3) Add 25g of Spirulina algae species to the culture solution formed in (2), then use an air pump to agitate the culture solution, light for 10 hours a day, the light intensity is about 50μmol / (㎡ S), and the temperature is controlled at 25 degrees Celsius;

[0027] (4) Check the pH value, when the pH value is between 10-12, press H 3 BO 3 -25g, MnCl 2 4H 2 O-20g, ZnSO 4 ·7H 2 O-3g, CuSO 4 ·5H 2 O-0.5g, Mo 7 o 24 6(NH 4 )·4H 2 O-0.15g, Co(NO 3 ) 2 ·6H 2 O-45mg order to add trace element supplements;

[0028]...

Embodiment 3

[0030] (1) Measure 10L of distilled water and place it in the culture machine;

[0031] (2) by FeSO 4 ·7H 2 O-0.2g, EDTA-0.5g, CaCl 2 2H 2 O-0.4g, MgSO 4 ·7H 2 O-4g, NaCl-3g, K 2 SO 4 -6g, KNO 3 -30g, K 2 HPO 4 -8g, NaHCO 3 - 130g is dissolved in water one by one, stirring and / or blowing while adding, each time after the previous component is dissolved, then put in the next component, and so on, until all the components are dissolved ;

[0032] (3) Add 25g of Spirulina algae species to the culture solution formed in (2), then use an air pump to agitate the culture solution, light for 10 hours a day, the light intensity is about 50μmol / (㎡ S), and the temperature is controlled at 25 degrees Celsius;

[0033] (4) Check the pH value, when the pH value is between 10-12, press H 3 BO 3 -30g, MnCl 2 4H 2 O-25g, ZnSO 4 ·7H 2 O-4g, CuSO 4 ·5H 2 O-0.6g, Mo 7 o 24 6(NH 4 )·4H 2 O-0.2g, Co(NO 3 ) 2 ·6H 2 0-50mg sequence of adding trace element supplements;

[...

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Abstract

The invention discloses a spirulina culture solution for a culture machine. The spirulina culture solution comprises a No.1 culture solution, a trace element replenishing solution and a No.2 replenishing solution. When spirulina is cultured in a commercial culture machine, the spirulina has the characteristics of being high in survival rate and high in growth rate of the spirulina. The No.2 replenishing solution is used, so that the culture period of the spirulina can be effectively prolonged. According to a formula, unessential components are removed, the consumption of dissolving componentsin the culture solution is reduced, and environment pollution caused by waste liquid obtained by culturing is also reduced.

Description

technical field [0001] The invention relates to a spirulina culture solution, in particular to a spirulina culture solution for a cultivation machine. Background technique [0002] Spirulina (scientific name: Spirulina) is a class of lower organisms, prokaryotes, filaments composed of unicellular or multicellular. It can reduce the toxic and side effects of cancer radiotherapy and chemotherapy, improve immune function, and reduce blood lipids. Its health benefits include: lowering cholesterol: lowering cholesterol can effectively prevent the onset of heart disease and stroke. The gamma-linolenic acid in spirulina It can reduce the cholesterol contained in the human body, thereby effectively reducing high blood pressure and preventing heart disease. Regulate blood sugar: Spirulina contains spirulina polysaccharide, magnesium, chromium and other hypoglycemic substances, which can regulate blood sugar metabolism through various ways (such as promoting insulin secretion, slowin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 彭义雄
Owner 深圳市太空微藻生物科技有限公司
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