A kind of tissue culture method for inducing the bulblets of Fritillaria japonicus

A technology of fritillary fritillaria and small bulbs, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of lack of research on rapid propagation technology, unfavorable heredity and genetic variation of fritillary fritillaria, and achieve shortened cultivation cycle, reducing the chance of genetic mutations, and the effect of good varieties

Active Publication Date: 2017-10-13
NANJING HAIYUAN CHINESE HERBAL PIECES CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are mainly two ways of in vitro rapid propagation of Fritillaria fritillaria: 1. Indirect organ generation: Callus induction by explants of Fritillaria fritillaria, and then induction of regenerated shoots is the most studied rapid propagation method at present, but Since this method needs to undergo cell dedifferentiation and redifferentiation to obtain tissue culture seedlings, there are a large number of genetic variations in this way, which is not conducive to the inheritance of the excellent quality of Fritillaria fritillaria.
2. The direct way of organ generation: this way does not go through the callus tissue, and the explants of Fritillaria fritillaria can directly induce adventitious buds or small bulbs, which can avoid the genetic variation caused by cell differentiation and redifferentiation, and is good for maintaining the germplasm of Fritillaria fritillaria. The traits have positive effects, but there is a lack of research on the rapid propagation of Fritillaria fritillaria through this method, and there are many deficiencies

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The present invention can be better understood from the following examples. However, those skilled in the art will readily understand that the specific material ratios, process conditions and results described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims . All technologies realized based on the above contents of the present invention belong to the scope of the present invention. Embodiment 1: A kind of method for inducing and cultivating the bulblets of Fritillaria japonica

[0022] (1) Explants are cut: select the bulbs of Fritillaria fritillaria without disease, peel off the scales, and cut the core buds of Fritillaria fritillaria from the base of the bulb;

[0023] (2) Sterilization: After rinsing with tap water for 15 minutes, put it into an ultra-clean workbench, sterilize it with 75% alcohol for 20 seconds, rinse it with sterile water for 3 times, and ...

Embodiment 2

[0027] Embodiment 2: A kind of new method of inducing and cultivating the bulblets of Fritillaria japonicus

[0028] (1) Explants are cut: select the bulbs of Fritillaria fritillaria without disease, peel off the scales, and cut the core buds of Fritillaria fritillaria from the base of the bulb;

[0029] (2) Sterilization: After rinsing with tap water for 15 minutes, put it into an ultra-clean workbench, sterilize it with 70% alcohol for 20 seconds, rinse it with sterile water for 5 times, and then sterilize it in 0.15% mercuric chloride for 15 minutes. Rinse with sterile water 6 times, dry the moisture on the explant surface with sterile filter paper;

[0030] (3) Seedling cultivation: Inoculate the sterilized explants in the seedling medium for cultivation, the seedling medium is MS+sucrose 30g / L+agar 6g / L, pH is 6.5, and the culture conditions are: temperature 25°C , the light intensity is 2500Lux, the light time is 14h, and the culture time is 25 days. After being cultiv...

Embodiment 3

[0033] Embodiment 3: A kind of new method of inducing and cultivating the bulblets of Fritillaria japonicus

[0034] (1) Explants are cut: select the bulbs of Fritillaria fritillaria without disease, peel off the scales, and cut the core buds of Fritillaria fritillaria from the base of the bulb;

[0035] (2) Sterilization: After rinsing with tap water for 15 minutes, put it into an ultra-clean workbench, sterilize it with 75% alcohol for 20 seconds, rinse it with sterile water for 4 times, and put it into 0.1% mercuric chloride to sterilize it for 10 minutes. Rinse 4 times with sterile water, and dry the moisture on the surface of the explants with sterile filter paper;

[0036] (3) Seedling cultivation: Inoculate the sterilized explants in the seedling medium for cultivation, the seedling medium is MS+sucrose 30g / L+agar 6g / L, pH is 6.5, and the culture conditions are: temperature 24°C , the light intensity is 3000Lux, the light time is 14h, and the culture time is 30 days. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention discloses a thunberg fritillary bulb bulblet inducement tissue culture method comprising the steps of cutting core buds of thunberg fritillary bulb to be used as explants, sterilization, seedling cultivation, induction culture, expansion cultivation and the like. For the first, the core buds of thunberg fritillary bulb are used as the explants for producing bulblets by induction after seedling by cultivation, the period for producing the bulblets of the thunberg fritillary bulb can be greatly shortened, the whole cultivation process is free of cell dedifferentiation and redifferentiation, the chance of genetic mutation is greatly reduced, and good quality can be guaranteed. The thunberg fritillary bulb bulblet inducement tissue culture method has rapid propagation speed, short culture period, and significant economic benefits popularization and application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for tissue culture and propagation of Fritillaria fritillaria, and is a new method for inducing and cultivating bulblets of Fritillaria fritillata. Background technique [0002] Fritillaria thunbergii (Fritillaria thunbergii) is one of the bulk of traditional Chinese medicinal materials commonly used in clinical practice. It is listed as the first of the "Eight Flavors of Zhejiang". At present, in the production of Fritillaria fritillaria, there are mainly 4 cultivars: narrow-leaf species, broad-leaf species, multi-seed species and small three sons. Among them, the narrow-leaf variety Fritillaria fritillaria has the most extensive planting area, and was identified as a new variety in 2007, named Zhebei No. 1; the wide-leaved variety Fritillaria fritillaria was identified as Zhebei No. 2 in 2014. Since the seeds of Fritillaria fritillaria are harvested, it takes 5 years for ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 蔡宝昌张彦南金俊杰秦昆明陈丹妮黄雨婷范孟雪郑艳萍陈林伟王彬
Owner NANJING HAIYUAN CHINESE HERBAL PIECES CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products