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Real-time fluorescence quantitative RT-PCR detection kit for mRNA of human BCSG1 (breast cancer-specific gene 1)

A technology of real-time fluorescence quantification and detection kit, applied in the field of biomedicine, can solve the problems of low positive rate of circulating breast tumor cells and no clinical application value, and achieve good stability

Inactive Publication Date: 2018-06-01
任秀敏
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Problems solved by technology

[0005] Since the current real-time fluorescent quantitative PCR technology is used to detect the expression of human breast cancer specific gene 1 (BCSG1) mRNA to judge the positive rate of circulating breast tumor cells is low, which has no clinical application value. Therefore, it is necessary to develop a high specificity, Sensitivity, accuracy and quality control BCSG1 mRNA real-time fluorescent quantitative RT-PCR detection kit is very necessary

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  • Real-time fluorescence quantitative RT-PCR detection kit for mRNA of human BCSG1 (breast cancer-specific gene 1)

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Embodiment Construction

[0021] In order to further understand the present invention, the preferred embodiments of the present invention are described below in conjunction with examples, but it should be understood that these descriptions are only to further illustrate the features and advantages of the present invention, rather than limiting the claims of the present invention.

[0022] 1. Human breast cancer specific gene 1 mRNA real-time fluorescent quantitative RT-PCR detection kit of the present invention

[0023] The kit of the present invention comprises erythrocyte lysate (made of pH7.6 0.01 mol / L Tris-HCl, 0.01 mol / L NaCl and 0.005 mol / L MgCl 2 RNase-free water, RNA extraction solution, dNTP mixture, reverse transcriptase storage solution, reverse transcription buffer, PCR reaction buffer, UNG enzyme storage solution, primer pairs, probes, standards (using BCSG1 plasmid TE solution was dissolved at a concentration of 2 × 10 7 copy number / μL), positive quality control (total RNA containing BC...

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Abstract

The invention belongs to the technical field of biomedicine and relates to a real-time fluorescence quantitative RT-PCR detection kit for mRNA of a human BCSG1 (breast cancer-specific gene 1). The real-time fluorescence quantitative RT-PCR detection kit for mRNA of the human BCSG1 comprises a red blood cell lysis buffer, RNase-free water, RNA extract, a dNTP mixture, a reverse transcriptase storage liquid, a reverse transcription buffer, a PCR buffer, a UNG (Uracil-N-Glycosylase) storage liquid, a primer pair, a probe, a standard, a positive quality control material and a negative quality control material. The real-time fluorescence quantitative RT-PCR detection kit for mRNA of the human BCSG1 has the advantages of higher specificity, sensitivity, accuracy, quality control and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a real-time fluorescent quantitative RT-PCR detection kit for human breast cancer specific gene 1mRNA. Background technique [0002] Breast cancer is a common malignant tumor that seriously threatens women's life and health. In recent years, the incidence of breast cancer in my country has increased significantly. [0003] At present, the incidence rate of breast cancer in my country is 42.55 / 100,000, ranking first among female malignant tumors. At present, there are many treatment methods for breast cancer, such as surgery, radiotherapy, chemotherapy, and biological therapy. Among them, surgical resection of breast cancer is the main treatment measure, but postoperative recurrence and metastasis have become the main reasons hindering the improvement of patient survival. Hematogenous dissemination is one of the main ways of tumor metastasis. Due to natural or invasive iatroge...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158
Inventor 任秀敏
Owner 任秀敏