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STED parallel microscopic imaging system based on structural light illumination

A technology of structured light illumination and microscopic imaging, which is applied in the direction of microscope, optics, optical components, etc., can solve the problems of lower effective fluorescence signal-to-noise ratio, higher complexity of imaging system, and influence on imaging resolution, etc., to expand the field of view Effect of range and imaging speed

Active Publication Date: 2018-06-05
SUZHOU GUOKE MEDICAL TECH DEV CO LTD +1
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Problems solved by technology

However, due to the addition of two-dimensional structured light, the complexity of the imaging system becomes higher, and the excitation light in this technology does not use structured light mode but uses wide-field excitation, which significantly reduces the signal-to-noise ratio of effective fluorescence and affects the imaging resolution.

Method used

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  • STED parallel microscopic imaging system based on structural light illumination
  • STED parallel microscopic imaging system based on structural light illumination
  • STED parallel microscopic imaging system based on structural light illumination

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Embodiment Construction

[0029] Please refer to figure 1 , is a STED parallel microscopic imaging system 100 based on structured light illumination provided by an embodiment of the present invention, an illumination module 110 , a detection module 120 , a control module 130 and an image reconstruction module 140 . in:

[0030] The illumination module 110 includes: an excitation light laser 111, a loss light laser 112, a liquid crystal spatial light modulator 113, a Wollaston prism 114, a first lens 115, a through-hole mask 116, a second lens 117, a first Half-wave plate 118, second half-wave plate 119, mirror 1110, first dichroic mirror 1111, second dichromatic mirror 1112, tube lens 1113, objective lens 1114 and three-dimensional nano-displacement stage 1115, described three-dimensional nano-displacement stage 1115 It can move in the XYZ three-dimensional direction, and the three-dimensional nano-displacement platform carries the sample to be detected.

[0031] The detection module 120 includes a f...

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Abstract

The invention provides an STED parallel microscopic imaging system based on structural light illumination. Through designing an illuminating module, one laser beam which is transmitted from an existing laser is divided into two coherent light beams; one loss light beam which is transmitted from a loss light laser is divided into two coherent light beams; exciting structural light and loss structural light which are uniformly distributed are respectively obtained through interference; the exciting structural light and the loss structural light respectively perform excitation and loss on a sample; STED parallel microscopic imaging is performed; then image reconstruction is performed through an STED coordinate positioning method and an SIM frequency domain frequency spectrum graph fusion method, thereby realizing super-resolution imaging and facilitating improvement of visual field range and imaging speed of an STED microscopic system.

Description

technical field [0001] The invention relates to the field of design and manufacture of microscopic detection instruments, in particular to a STED parallel microscopic imaging system based on structured light illumination. Background technique [0002] Super-resolution microscopic imaging technology is a cutting-edge technology used in biological and other research fields. It is mainly divided into two types of microscopic imaging methods: coordinate positioning and coordinate random. Among them, the typical representative of the coordinate positioning microscopic imaging method is stimulated radiation depletion (STED) microscopic imaging. STED microscopic imaging directly uses optical methods to scan the sample point by point to achieve super-resolution imaging. However, limited by the single-point scanning method, the imaging speed of STED microscopic imaging is relatively slow. [0003] In recent years, in order to improve the imaging speed of STED microscopic imaging tec...

Claims

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Application Information

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IPC IPC(8): G02B21/00G02B21/06
CPCG02B21/0032G02B21/06
Inventor 唐玉国张运海肖昀
Owner SUZHOU GUOKE MEDICAL TECH DEV CO LTD
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