Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of delta-amyrone or extract with delta-amyrone as main component to preparation of medicine for protecting liver damage

A technology for balsam resin and extract, which is applied in the field of traditional Chinese medicine extracts, and achieves the effects of good protection, simple and easy preparation method, and small toxic and side effects.

Inactive Publication Date: 2018-06-08
SOUTHWEST UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about whether the low-polarity solvent of weeping grass, such as dichloromethane extract, has hepatoprotective activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of delta-amyrone or extract with delta-amyrone as main component to preparation of medicine for protecting liver damage
  • Application of delta-amyrone or extract with delta-amyrone as main component to preparation of medicine for protecting liver damage
  • Application of delta-amyrone or extract with delta-amyrone as main component to preparation of medicine for protecting liver damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of embodiment 1, weeping pot grass dichloromethane extract

[0027] Wash the dried whole herb of C. chinensis, soak it in ethanol with a volume fraction of 95% for one day, and then extract it by diafiltration three times. The medicinal liquids extracted in the three times are combined. Concentrate under pressure to obtain an extract, the extract is passed through a silica gel column, first eluted with petroleum ether until colorless, and then eluted with dichloromethane until colorless, collecting the dichloromethane eluate, and recovering the dichloromethane organic solution under reduced pressure, The concentrated extract is freeze-dried to obtain the dichloromethane extract of C. chinensis.

Embodiment 2

[0028] Embodiment 2, the preparation of compound δ-amyrone

[0029] Wash the dried whole herb of C. chinensis, soak it in ethanol with a volume fraction of 95% for one day, and then extract it by diafiltration three times. The medicinal liquids extracted in the three times are combined. Concentrate under pressure to obtain an extract, the extract is passed through a silica gel column, first eluted with petroleum ether until colorless, and then eluted with dichloromethane until colorless, collect the dichloromethane eluate, the eluate has precipitates, filter and discard Remove the filtrate, and the precipitate is the active ingredient.

[0030] δ-Amyrone: white powder; 13C NMR (100MHz, CDCl 3 )δ39.3(C-1), 34.1(C-2), 218.1(C-3), 47.2(C-4), 54.8(C-5), 19.8(C-6), 26.5(C-7 ),40.9(C-8),50.0(C-9),36.9(C-10),22.2(C-11),26.5(C-12),134.0(C-13),44.7(C-14) ,25.0(C-15),36.5(C-16),34.5(C-17),133.6(C-18),39.6(C-19), 33.3(C-20),35.4(C-21), 38.7 (C-22), 21.0 (C-23), 26.9 (C-24), 16.2 (C-...

Embodiment 3

[0032] Example 3, the research on the hepatoprotective effect of the dichloromethane extract of the weeping pot grass

[0033] 1. Animal grouping, modeling, and drug administration methods

[0034] Get 60 SD rats, after 3 days of laboratory adaptive feeding, divide them into 6 groups according to body weight: blank group, model group ANIT (70mg / kg), positive drug bifendate group (150mg / kg), low dose Drug group 100mg / kg, medium dose administration group 200mg / kg, high dose administration group 400mg / kg, after adaptive feeding for 3 days, start administration on the 4th day (note: before the first administration, fasting and drinking 24h), the animals in each group were intragastrically administered twice a day (once every 12h), for 5 consecutive days or a week, and 1 hour after the last administration, except the blank group, each group of rats was intraperitoneally injected with ANIT (70mg / kg) to prepare Rat liver injury model, 12 hours after administration of the model, bloo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
quality scoreaaaaaaaaaa
Login to View More

Abstract

The invention discloses application of delta-amyrone or an extract with the delta-amyrone as a main component to preparation of a medicine for protecting liver damage. The extract with the delta-amyrone as the main component is a dichloromethane extract of Sedum sarmentosum Bunge, and an extraction method thereof comprises the following steps: cleaning the dry whole Sedum sarmentosum Bunge, extracting with ethanol with the volume fraction of 95% according to the ethanol volume being equivalent to 6 times of the weight of a medicinal material during extraction, concentrating into an extract, enabling the extract to pass through a silica gel column, firstly eluting with petroleum ether until a colorless state, then eluting with dichloromethane until a colorless state, collecting a dichloromethane eluate, recycling an organic dichloromethane solution, and performing freeze drying on the concentrated extract to obtain the dichloromethane extract of the Sedum sarmentosum Bunge. The invention finds for the first time that the dichloromethane extract of the Sedum sarmentosum Bunge has hepatoprotective activity, which is of a great significance in further development and utilization of theSedum sarmentosum Bunge.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, specifically the application of delta-amyrone (delta-amyrone) or an extract with delta-amyrone as the main component in the preparation of a drug for protecting liver damage, with delta-amyrone as the main component The extract is prepared from Sedum genus plants. Background technique [0002] my country is a country with a high incidence of liver disease. Now various environmental factors and bad living habits make the incidence of various liver diseases such as hepatitis, liver cirrhosis, fatty liver, and liver cancer continue to rise. How to effectively prevent and treat liver disease has become an important issue. One of the challenges facing the medical profession. Many liver diseases have a common pathological state, which is liver damage. Chemical poisons, hepatotoxic drugs, viral infections, alcohol and other harmful factors may cause abnormal liver function and cause liver damag...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/56C07J63/00A61K36/41A61P1/16
CPCA61K31/56A61K36/41A61K2236/333A61K2236/51A61K2236/55C07J63/008
Inventor 王国伟张小龙陈敏
Owner SOUTHWEST UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com