Application of atherosclerotic renal artery stenosis diagnosis molecular marker

A technique for atherosclerosis and molecular markers, applied in the application field of molecular markers for the diagnosis of atherosclerotic renal artery stenosis

Active Publication Date: 2018-06-12
GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no data showing that there are specific markers in patients with renal artery ...

Method used

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  • Application of atherosclerotic renal artery stenosis diagnosis molecular marker
  • Application of atherosclerotic renal artery stenosis diagnosis molecular marker
  • Application of atherosclerotic renal artery stenosis diagnosis molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024]Example 1 (screening and identification of lncRNA specifically expressed in human renal artery tissue)

[0025] Use "LncPath TM Human Cardiovascular Disease Pathway” (Shanghai Kangcheng Biological Co., Ltd.) screened 4 pairs of human renal artery tissues with renal artery stenosis and non-renal artery stenosis, and screened out significantly differentially expressed LncRP11-387H17.6, BC080653, RP1 through chip screening technology -32B1.4, GHRLOS, RP5-1068H6.3 and XLOC_009769. Such as figure 1 Shown, (A) Scatter plot of lncRNAs expression in renal artery stenosis tissue and non-renal artery stenosis tissue. The X-axis represents the data of the non-renal artery stenosis group. The Y axis represents the data of renal artery stenosis tissue. Points above and below the green line are multiples of difference ≥ 1.5, "Test" represents renal artery stenosis tissue; "Ctrol" represents non-renal artery stenosis tissue. (B) Heat map of expression of lncRNAs analyzed by microa...

Embodiment 2

[0027] 1. Sample source:

[0028] From January 2015 to October 2016, the peripheral blood of patients undergoing coronary angiography and renal angiography in Shenyang Military Region General Hospital was collected, and 18 cases of renal artery stenosis and 18 cases of non-renal artery stenosis were randomly selected as training samples; 99 cases of renal artery stenosis were selected as training samples. , 49 cases of non-renal artery stenosis were used as validation samples. The peripheral blood of 18 healthy volunteers was taken from the physical examination center as training samples, and the peripheral blood of 50 healthy volunteers was used as verification samples. Extract peripheral blood from different individuals, collect it in EDTA anticoagulant tubes, centrifuge at 3000g at 4°C for 10 minutes to remove plasma, add 5ml of erythrocyte lysate, shake well, let stand for 20 minutes, then centrifuge at 3000g at 4°C for 10 minutes , discarded to remove cell debris, the ab...

Embodiment 4

[0063] The expression distribution of leukocyte lncRNA in the peripheral blood of embodiment 4

[0064] As a good disease diagnostic marker, the first prerequisite is that it is highly expressed in people with renal artery stenosis, and it cannot be detected or exists relatively stably in the peripheral blood of healthy people. Therefore, the present invention first detects the renal artery tissue of 4 patients with renal artery stenosis and non-renal artery stenosis by chip method, wherein there are about 1150 lncRNAs that can be detected by the lncRNA chip in the renal artery stenosis tissue, according to the difference > 3 times, P figure 2 shown.

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Abstract

The invention relates to the technical field of molecular biological medical treatment, in particular to application of an atherosclerotic renal artery stenosis diagnosis molecular marker. The molecular marker is PR11-387H17.6, and the nucleotide sequence of the molecular marker is shown in SEQ ID No.1. The purpose is to provide the atherosclerotic renal artery stenosis diagnosis molecular markerPR11-387H17.6 aiming at the fact that currently and clinically, detection of atherosclerotic renal artery stenosis is lack of a diagnosis index with high sensitivity and high specificity. The application is significant in finding the atherosclerotic renal artery stenosis diagnosis molecular marker with high sensitivity and high specificity and developing a corresponding assisting diagnosis reagentor kit.

Description

technical field [0001] The invention relates to the technical field of molecular biomedicine, in particular to the application of molecular markers for the diagnosis of atherosclerotic renal artery stenosis. Background technique [0002] Long non-coding RNA (LncRNA) is a kind of non-coding RNA molecule with a transcript length of more than 200nt in eukaryotic cells, which has no or little protein coding function and is commonly transcribed in mammalian genomes. LncRNAs were once considered to be by-products of the transcription process and have no biological function. At present, more and more studies have confirmed that LncRNA has complex biological functions, participates in important links in signal transduction processes such as genome imprinting, transcription control, post-transcriptional regulation, and protein function regulation, and is closely related to the occurrence of human diseases. relation. [0003] LncRNAs are distributed in the cytoplasm, nucleus, and or...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 王效增傅文霞张效林张权宇
Owner GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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