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Water-in-oil adjuvant for animal vaccine and preparation method and use thereof

A water-in-oil vaccine technology, applied in vaccines, veterinary vaccines, medical preparations containing active ingredients, etc., can solve the problems of difficult distribution and metabolism, difficult preparation technology, poor vaccine stability, etc., and achieve low price, Raw materials are readily available and the effect of reducing production costs

Active Publication Date: 2021-07-27
四川诺顺科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with non-ionic surfactants, ionic surfactants have the following defects: (1) easily ionized in the water phase, and precipitate with other substances, resulting in difficult distribution and metabolism; (2) certain cytotoxicity
However, its preparation technology is difficult and the vaccine stability is poor

Method used

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  • Water-in-oil adjuvant for animal vaccine and preparation method and use thereof
  • Water-in-oil adjuvant for animal vaccine and preparation method and use thereof
  • Water-in-oil adjuvant for animal vaccine and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0046] The raw and auxiliary materials, reaction tanks, filtering utensils used in the following examples and the utensils in direct contact with the raw materials and the resulting products all meet the medical requirements; the inspection of raw and auxiliary materials and packaging materials should meet the requirements; before the production operation, the reaction tank and Clean the appliances that directly contact raw materials and products; control the production environment in the process of adjuvant preparation to meet the requirements; after production, clean the reaction tank and the appliances that directly contact raw materials and products; The environment is clean to meet requirements.

Embodiment 1

[0048] The raw materials of water-in-oil adjuvant for animal vaccine prepared in this embodiment are calculated by volume fraction: 76.5% of mineral oil, 13.0% of Span 80, 2.0% of Tween 80, 3.0% of propylene glycol fatty acid ester, 3.5% of hydroxylated lanolin, Glyceryl monostearate 1.5%, polyoxyethylene (2EO) oleyl ether 0.5%. The mineral oil is white oil of injection grade, and the emulsifier is a pharmaceutical grade nonionic surfactant.

[0049] The water-in-oil adjuvant for animal vaccines in this example is prepared according to the volume fraction of the above raw material components through the following steps:

[0050] (1) Mineral oil is added in the reaction tank and stirred, the mineral oil is heated to 35 DEG C under stirring condition, the rotating speed of stirring is controlled as 90 rpm, and the stirring time is 30 minutes;

[0051] (2) Add the weighed Span 80, propylene glycol fatty acid ester, and hydroxylated lanolin successively into the reaction tank and...

Embodiment 2

[0055] The raw materials of water-in-oil adjuvant for animal vaccine prepared in this embodiment are calculated by volume fraction: 75.0% of mineral oil, 13.5% of Span 80, 2.5% of Tween 80, 3.0% of propylene glycol fatty acid ester, 3.0% of hydroxylated lanolin, Glyceryl monostearate 2.0%, polyoxyethylene (2EO) oleyl ether 1.0%. The mineral oil is white oil of injection grade, and the emulsifier is a pharmaceutical grade nonionic surfactant.

[0056] The water-in-oil adjuvant for animal vaccines in this example is prepared according to the volume fraction of the above raw material components through the following steps:

[0057] (1) Mineral oil is added in the reaction tank and stirred, the mineral oil is heated to 40 DEG C under stirring condition, the rotating speed of stirring is controlled as 120 rpm, and the stirring time is 20 minutes;

[0058] (2) Add the weighed Span 80, propylene glycol fatty acid ester, and hydroxylated lanolin successively into the reaction tank an...

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Abstract

The invention discloses a water-in-oil adjuvant for animal vaccines and its preparation method and application. The water-in-oil adjuvant for animal vaccines is mainly prepared from the following raw materials in terms of volume fraction: mineral oil: 74.5%-76.5% %; Span 80: 13.0%‑15.5%; Tween 80: 1.5%‑2.5%; Propylene Glycol Fatty Acid Ester: 2.5%‑3.0%; Hydroxylated Lanolin: 2.5%‑3.5%; Glyceryl Monostearate : 1.5%‑2.0%; polyoxyethylene (2EO) oleyl ether: 0.5%‑1.0%. Using the adjuvant provided by the present invention, a water-in-oil vaccine can be prepared by a one-step emulsification method, the production process is simple, the cost is low, and the obtained water-in-oil vaccine is safe, effective, low in viscosity, small in side effects, and good in stability. The safety inspection and efficacy inspection all meet the relevant requirements of the "Chinese Veterinary Pharmacopoeia".

Description

technical field [0001] The invention belongs to the field of biological product animal vaccines, and relates to a water-in-oil adjuvant for animal vaccines, a preparation method and application thereof. Background technique [0002] Oil-emulsion adjuvant for animal vaccines is a substance that is used prior to or simultaneously with antigens to enhance the body's non-specific immunity and the immunogenicity of antigens, but does not have antigenic properties itself. The oil-emulsion adjuvant vaccine is a vaccine that uses mineral oil and emulsifiers as immune enhancers. This type of adjuvant can make the antigen release continuously and stably at the injection site, provide a carrier for the antigen transport in the lymphatic system, increase the formation of monocytes and Aggregation, improving the immune efficacy of vaccines, is one of the most popular types of vaccines in the domestic and foreign vaccine markets. Oil-emulsion adjuvant vaccines can be divided into three do...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39
CPCA61K39/39A61K2039/552A61K2039/55511A61K2039/55566A61K2039/55588
Inventor 吴福文扶星星王永胜汪小芳
Owner 四川诺顺科技有限公司