Method for typing genes associated with folic acid metabolism and kit

Abstract

The invention discloses a method for typing genes associated with folic acid metabolism and a kit. The method comprises the following steps: amplifying: amplifying a sample DNA by using a PCR primer to obtain a PCR product and purifying the PCR product, wherein the PCR primer comprises nucleotide sequences as shown as SEQ ID NO: 1 to SEQ ID NO: 6; extending: performing single base extension on thepurified PCR product by using an extension primer SEQ ID NO: 9 for a 1298th site of MTHFR, an extension primer SEQ ID NO: 10 for a 677th site of the MTHFR and an extension primer SEQ ID NO: 11 for a66th site of MTRR and purifying an extension product; performing flight mass spectrometry: transferring the purified extension product to a mass spectrometry chip for mass spectrometry. According to the technical scheme, multiple detection of a reaction is achieved, the reagent cost and other cost such as manpower cost, disposable consumable cost and laboratory operation cost are greatly reduced,and the throughput of a detected sample is greatly increased.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a genotyping method and kit related to folic acid metabolism. Background technique [0002] Folic acid is a B vitamin, which is an essential substance for cell growth and tissue repair, and an indispensable nutrient for embryonic development. The human body cannot synthesize folic acid itself, and the folic acid ingested from food can only participate in many important biochemical metabolic reactions of the human body if it is reduced to tetrahydrofolate under the action of folate reductase. In addition to preventing fetal neural tube defects, the clinical function of folic acid can also reduce the incidence of pregnancy-induced hypertension, spontaneous abortion, intrauterine growth retardation, premature delivery, and low birth weight in newborns. [0003] my country is a country with a high incidence of birth defects. According to the data released by the former Ministry of Healt...

AI Technical Summary

Problems solved by technology

These methods either have limited detection capabilities, or are time-consuming and labor-intensive. More importantly, these methods cannot simultaneously perform high-throughput detection of multiple mutation sites in different genes.

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