Method for typing genes associated with folic acid metabolism and kit

A technology for genotyping and folic acid metabolism, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as inability to perform high-throughput detection, time-consuming and labor-intensive, and limited detection capabilities.

Inactive Publication Date: 2018-06-15
深圳中南医学检验实验室
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  • Abstract
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Problems solved by technology

These methods either have limited detection capabilities, or are time-consuming and labor-intensive. More importan...

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  • Method for typing genes associated with folic acid metabolism and kit
  • Method for typing genes associated with folic acid metabolism and kit
  • Method for typing genes associated with folic acid metabolism and kit

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Embodiment Construction

[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0033] In addition, the technical solutions of the various embodiments can be combined with each other, but it must be based on the realization of those skilled in the art. When the combination of technical solutions is contradictory or cannot be realized, it should be considered that the combination of technical solutions does not exist , nor within the scope of protection required by the present invention.

[0034] Two genes closely related to folat...

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Abstract

The invention discloses a method for typing genes associated with folic acid metabolism and a kit. The method comprises the following steps: amplifying: amplifying a sample DNA by using a PCR primer to obtain a PCR product and purifying the PCR product, wherein the PCR primer comprises nucleotide sequences as shown as SEQ ID NO: 1 to SEQ ID NO: 6; extending: performing single base extension on thepurified PCR product by using an extension primer SEQ ID NO: 9 for a 1298th site of MTHFR, an extension primer SEQ ID NO: 10 for a 677th site of the MTHFR and an extension primer SEQ ID NO: 11 for a66th site of MTRR and purifying an extension product; performing flight mass spectrometry: transferring the purified extension product to a mass spectrometry chip for mass spectrometry. According to the technical scheme, multiple detection of a reaction is achieved, the reagent cost and other cost such as manpower cost, disposable consumable cost and laboratory operation cost are greatly reduced,and the throughput of a detected sample is greatly increased.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a genotyping method and kit related to folic acid metabolism. Background technique [0002] Folic acid is a B vitamin, which is an essential substance for cell growth and tissue repair, and an indispensable nutrient for embryonic development. The human body cannot synthesize folic acid itself, and the folic acid ingested from food can only participate in many important biochemical metabolic reactions of the human body if it is reduced to tetrahydrofolate under the action of folate reductase. In addition to preventing fetal neural tube defects, the clinical function of folic acid can also reduce the incidence of pregnancy-induced hypertension, spontaneous abortion, intrauterine growth retardation, premature delivery, and low birth weight in newborns. [0003] my country is a country with a high incidence of birth defects. According to the data released by the former Ministry of Healt...

Claims

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Application Information

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IPC IPC(8): C12Q1/6858C12Q1/6883
CPCC12Q1/6858C12Q1/6883C12Q2600/156C12Q2600/16C12Q2531/113C12Q2533/101C12Q2537/143C12Q2565/627
Inventor 宁紫妍杨连兴朱峰王赫群
Owner 深圳中南医学检验实验室
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