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Method for quick selection of sheath blight resistant rice carrying qSBRLEYD10A gene

A technology for rapid selection and sheath blight, applied in plant genetic improvement, botany equipment and methods, biochemical equipment and methods, etc., can solve problems such as poor accuracy, low accuracy of inoculation identification results, and large error in results

Inactive Publication Date: 2018-06-19
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because most of the traditional inoculation identification is carried out under field planting conditions, the results of inoculation identification have large errors and poor accuracy, and there are differences in the inoculation results of different years, resulting in low accuracy of inoculation identification results

Method used

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  • Method for quick selection of sheath blight resistant rice carrying qSBRLEYD10A gene
  • Method for quick selection of sheath blight resistant rice carrying qSBRLEYD10A gene
  • Method for quick selection of sheath blight resistant rice carrying qSBRLEYD10A gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Example 1: Application of D1042 marker in F6 generation segregation population of rice variety Lemont and rice variety Yangdao No. 4 hybrid combination.

[0008] 1. The rice variety Lemont was used as the female parent, and the rice variety Yangdao No. 4 was used as the male parent to cross and construct the F6 generation segregation population (Lemont is a japonica rice variety from the United States, Yangdao No. 4 is an indica rice variety from Jiangsu, Lemont and Yangdao 4 was provided by the National Rice Germplasm Resource Bank of China Rice Research Institute), this F6 segregation population including 219 lines was sown in May 2013 at the China Rice Research Institute Experimental Field in Fuyang District, Hangzhou.

[0009] 2. Use the marker D1042 to perform PCR detection on each strain in the segregation population of 219 strains, and use the characteristics of the linkage between the marker D1042 and the gene qSBRLEYD10A to detect which strain carries the diseas...

Embodiment 2

[0013] Example 2: Application of D1042 marker in F9 generation segregation population of rice variety Lemont and rice variety Yangdao No. 4 hybrid combination.

[0014] 1. The rice variety Lemont was used as the female parent, and the rice variety Yangdao No. 4 was used as the male parent to cross and construct the F9 generation segregation population (Lemont is a japonica rice variety from the United States, Yangdao No. 4 is an indica rice variety from Jiangsu, Lemont and Yangdao 4 was provided by the National Rice Germplasm Resource Bank of China Rice Research Institute), this F9 segregation population including 219 lines was sown in May 2015 at the Experimental Farm of China Rice Research Institute in Fuyang District, Hangzhou.

[0015] 2. Use the marker D1042 to perform PCR detection on each strain in the segregation population of 219 strains, and use the characteristics of the linkage between the marker D1042 and the gene qSBRLEYD10A to detect which strain carries the dise...

Embodiment 3

[0019] Example 3: Application of the D1042 marker in the segregated population of the F11 generation of the hybrid combination of the rice variety Lemont and the rice variety Yangdao No. 4.

[0020] 1. The rice variety Lemont was used as the female parent, and the rice variety Yangdao No. 4 was used as the male parent to cross and construct the F11 generation segregation population (Lemont is a japonica rice variety from the United States, Yangdao No. 4 is an indica rice variety from Jiangsu, Lemont and Yangdao 4 was provided by the National Rice Germplasm Resource Bank of China Rice Research Institute), this F11 segregation population including 219 lines was sown in May 2016 at the Experimental Farm of China Rice Research Institute in Fuyang District, Hangzhou.

[0021] 2. Use the marker D1042 to perform PCR detection on each strain in the segregation population of 219 strains, and use the characteristics of the linkage between the marker D1042 and the gene qSBRLEYD10A to dete...

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Abstract

The invention discloses a method for quick selection of sheath blight resistant rice carrying qSBRLEYD10A gene. The method utilizes the D1042 marker for PCR amplification of different strains in a Lemont and Yangdao 4 combined recombinant inbred line segregation population, and selects strains with banding patterns consistent with that of the parent Lemont, thus obtaining strains carrying qSBRLEYD10A sheath blight resistant allelic genes. Compared with the traditional field inoculation identification method, the method provided by the invention is simpler and quicker, can select out a sheath blight resistant rice material carrying qSBRLEYD10A gene without inoculation identification, has selection accuracy higher than the field inoculation method, and can greatly improve the breeding efficiency.

Description

technical field [0001] The invention relates to a method for quickly selecting sheath blight-resistant rice carrying qSBRLEYD10A gene by using D1042 markers. The method can quickly select stable rice sheath blight-resistant strains by using D1042 markers. Background technique [0002] Rice is an important food crop related to my country's food security. The traditional methods of breeding sheath blight-resistant rice in my country's rice breeding community are all through phenotypic selection, that is, inoculating and identifying rice plants with the pathogenic bacteria of sheath blight at different rice developmental stages. Because most of the traditional inoculation identification is carried out under field planting conditions, the results of inoculation identification have large errors and poor accuracy, and the inoculation results of different years are different, resulting in low accuracy of inoculation identification results. The selection of rice sheath blight resis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895A01H1/02A01H1/04
CPCC12Q1/6895A01H1/02A01H1/04C12Q2600/13
Inventor 陈渊杨长登曾宇翔季芝娟梁燕
Owner CHINA NAT RICE RES INST