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Application of scopoletin to preparation of medicine for inhibiting neuroinflammation

A technology of scopolamine and neuroinflammation, which is applied in the application field of scopolamine in the preparation of neuroinflammation-inhibiting drugs, can solve the problems of no scopolamine neuroinflammation, etc., and achieve significant neuroinflammation activity, high clinical application value and Development prospect, effect of suppressing neuroinflammation activity

Inactive Publication Date: 2018-06-22
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of scopolamine in the prevention and treatment of neuroinflammation-related diseases

Method used

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  • Application of scopoletin to preparation of medicine for inhibiting neuroinflammation
  • Application of scopoletin to preparation of medicine for inhibiting neuroinflammation
  • Application of scopoletin to preparation of medicine for inhibiting neuroinflammation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Griess colorimetric method to investigate the inhibitory effect of scopolamine on the release of nitric oxide (NO) from microglia induced by LPS;

[0032] Cell line: mouse microglial cell line BV-2;

[0033] Drugs: LPS; Scopolamine; Minocycline (MINO).

[0034] method:

[0035] (1) Culture of mouse microglial cell line BV-2:

[0036] The cell culture solution was prepared based on DMEM medium, containing 10% FBS, 100 U penicillin and 100 U streptomycin, and 50 μM 2-mercaptoethanol (all final concentrations). at 5% CO 2 , under the condition of 37 ℃, the BV-2 microglial cells were divided into about 4×10 5 The cell density of cells / ml was cultured in a cell culture incubator, and the growth of the cells was observed regularly. When the area of ​​the cell adherence accounted for about 50-60% of the bottom area of ​​the culture flask, the cells were digested with trypsin and passaged, and then continued to culture. Take 3-7 generations of cells for experiments.

[00...

Embodiment 2

[0044] DCFH oxidation was used to investigate the effect of scopolamine on the release of reactive oxygen species (ROS) from microglia induced by LPS;

[0045] Cell line: mouse microglial cell line BV-2;

[0046] Drugs: LPS; Scopolamine; MINO.

[0047] Experimental principle: DCFH-DA (2′,7′-dichlorodihydrofluororescein diacetate) can enter the cell and undergo a deacetylation reaction to generate DCFH. DCFH is a non-fluorescent substance, which can be oxidized by intracellular ROS to generate fluorescent substance DCF, and the amount of ROS release can be reflected by detecting the fluorescence intensity. The specific operation steps are as follows: DCFH-DA was dissolved in pure methanol to prepare a mother solution with a concentration of 10 mM, and the mother solution was diluted 500 times with Hank's balanced salt solution (HBSS) to a final concentration of 20 μM before use. Take the BV-2 cells after drug treatment, suck the supernatant, incubate with the 20 μM DCFH-DA so...

Embodiment 3

[0051] The effects of scopolamine on the release of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) from microglial cells induced by LPS were investigated by ELISA;

[0052] Cell line: mouse microglial cell line BV-2;

[0053] Drugs: LPS; Scopolamine; MINO.

[0054] Detection method: Take BV-2 cells in the logarithmic growth phase, inoculate the cells in a 6-well plate with fresh serum-free DMEM medium, and the cell density is 3×10 5 cells / ml, inoculum volume 600μl / well, placed in an incubator at 37°C, 5% CO 2 cultivated under conditions. After the cells adhered to the wall for 3 hours, the culture solution was carefully sucked out, and according to the experimental group, the cells were replaced with different drugs prepared in serum-free DMEM culture solution to incubate the cells. At the same time, a blank control group was set up, and each group was set with 3 replicate wells, and the drug addition to the cells continued. After culturing for 2-4 hours, collec...

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Abstract

The invention relates to application of scopoletin to inhibition of neuroinflammation and prevention and / or treatment of acute and chronic neurodegenerative diseases caused by the neuroinflammation, and in particular relates to application of the scopoletin to preparation of a medicine for inhibiting the neuroinflammation. The invention proves that the scopoletin has the remarkable neuroinflammation inhibition activity through a pharmacological experiment for the first time, and the scopoletin can be used for preparing the medicine for preventing and / or treating the neuroinflammation and related diseases, so that the scopoletin has relatively high clinical application value and development prospect. The invention provides novel application of the scopoletin to the inhibition of the neuroinflammation. The pharmacological experiment proves that the scopoletin can be used for remarkably inhibiting releasing of LPS (Lipopolysaccharide) induced microglial cell inflammation mediators NO, ROS(Reactive Oxygen Species), TNF-alpha (Tumor Necrosis Factor-alpha) and IL-1beta (Interleukin-1beta), and has the remarkable neuroinflammation inhibition activity. Therefore, the compound can be usedfor preparing the medicine for preventing and / or treating the neuroinflammation and related central nervous degenerative diseases.

Description

technical field [0001] The invention relates to the application of scopolamine in inhibiting neuroinflammation and preventing and / or treating acute and chronic neurodegenerative diseases caused by neuroinflammation, especially the application of scopolamine in the preparation of medicaments for inhibiting neuroinflammation. Background technique [0002] Neurodegenerative diseases (neurodegeneration disorders, ND) are a group of chronic progressive neurological diseases based on primary neuron degeneration, mainly including Alzheimer's disease (Alzheimer's disease, AD), Parkinson's disease (Parkinson's disease) , PD), multiple sclerosis (multiple sclerosis, MS), Huntington's disease (Huntington's disease, HD) and so on. With the aging of the world's population, the incidence of neurodegenerative diseases is on the rise. For example, the prevalence of AD in people over 60 years old in my country is 5.1%, and that in people over 85 years old is 30%. There are more than 8 millio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61P25/00A61P29/00A61P25/28A61P25/16A61P25/14
Inventor 李娟姚遥李玮琦
Owner NINGXIA MEDICAL UNIV
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