Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of clinical cord blood monocytes

A mononuclear cell and cell technology, applied in the biological field, can solve problems that do not meet clinical applications, and achieve the effects of avoiding acute renal failure, short time consumption, and low cost

Inactive Publication Date: 2018-06-22
重庆斯德姆生物技术有限公司
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, although various methods for preparing mesenchymal stem cells disclosed at present can obtain umbilical cord blood mononuclear cells (rich in hematopoietic stem cells), none of them meet the standards for clinical application. The process is clinically graded, unified, and systematically monitored to achieve clinical standard products of cord blood mononuclear cells (rich in hematopoietic stem cells)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of clinical cord blood monocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]This embodiment provides a method for preparing umbilical cord blood mononuclear cells for clinical use, which specifically includes the following steps:

[0039] Step 1: Preliminary screening and storage of umbilical cord blood: screening and collection of umbilical cord blood from healthy full-term maternal volunteers, and storing in blood bags at 7°C.

[0040] At the same time, a tube of maternal blood was collected for re-examination. The re-examination items were viral immunological detection and genetic detection of hepatitis B, hepatitis C, AIDS, and syphilis.

[0041] Step 2: Cultivation: put the umbilical cord blood sample in a culture dish containing RPMI-1640 medium, and incubate for 2 hours at 37° C. in a cell culture incubator containing 5% CO2.

[0042] Step 3: Absorption: After the monocytes adhere to the wall, discard the suspended cells in the upper layer, wash gently with PBS buffer twice, add a small amount of RPMI-1640 medium, and scrape off the adher...

Embodiment 2

[0048] This embodiment provides a method for preparing umbilical cord blood mononuclear cells for clinical use, which specifically includes the following steps:

[0049] Step 1: Preliminary screening and storage of umbilical cord blood: Screening and collection of umbilical cord blood from healthy full-term maternal volunteers, and storing in blood bags at 5°C.

[0050] At the same time, a tube of maternal blood was collected for re-examination. The re-examination items were viral immunological detection and genetic detection of hepatitis B, hepatitis C, AIDS, and syphilis.

[0051] Step 2: Cultivation: put the umbilical cord blood sample in a petri dish containing RPMI-1640 medium, and incubate for 3 hours at 37° C. in a cell culture incubator containing 6% CO2.

[0052] Step 3: Absorption: After the monocytes adhere to the wall, discard the suspended cells in the upper layer, wash gently with PBS buffer twice, add a small amount of RPMI-1640 medium, and scrape off the adhere...

Embodiment 3

[0058] This embodiment provides a method for preparing umbilical cord blood mononuclear cells for clinical use, which specifically includes the following steps:

[0059] Step 1: Preliminary screening and storage of umbilical cord blood: screening and collection of umbilical cord blood from healthy full-term maternal volunteers, and storing in blood bags at 6°C.

[0060] At the same time, a tube of maternal blood was collected for re-examination. The re-examination items were viral immunological detection and genetic detection of hepatitis B, hepatitis C, AIDS, and syphilis.

[0061] Step 2: Incubation: Place the cord blood sample in a petri dish containing RPMI-1640 medium at 37°C with 4% CO 2 Incubate for 1 h in a cell culture incubator.

[0062] Step 3: Absorption: After the monocytes adhere to the wall, discard the suspended cells in the upper layer, wash gently with PBS buffer twice, add a small amount of RPMI-1640 medium, and scrape off the adherent cells with a cell scr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of cord blood monocytes. The preparation method comprises the following steps: 1, conducting preliminary screening and preserving of umbilical cord blood;2, implementing cultivation; 3, implementing absorbing; 4, implementing collecting; 5, conducting cell culture; 6, freezing and detecting monocytes; and 7, using the monocytes. The preparation methodprovided by the invention tries to achieve unified and systematic monitoring over a whole process from raw material collection to completion of cell preparation, so that a standard product from separation and freezing of the cord blood monocytes is obtained.

Description

technical field [0001] The application relates to the field of biotechnology, in particular to a method for preparing umbilical cord blood mononuclear cells for clinical use. Background technique [0002] Monocytes can differentiate into dendritic cells and macrophages, and are important precursor cells for research related to dendritic cells and macrophages. Monocytes are differentiated from pluripotent hematopoietic stem cells, develop in the bone marrow, enter the blood, stay for 2 to 3 days, and then migrate to the surrounding tissues. Therefore, peripheral blood can be an important source of mononuclear cells. Typically, monocytes account for only 3% to 8% of peripheral blood cells, and independent cell lines are difficult to obtain. Currently, commonly used methods for isolating monocytes include wall-attachment method, immunomagnetic bead method and flow cytometry. Therefore, how to obtain ideal monocytes is the key to carry out related experimental research. [0...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0786A01N1/02
CPCC12N5/0645A01N1/0221
Inventor 赵翔李国焱唐玲熊华强
Owner 重庆斯德姆生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com