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Separation and extraction method of amino acid in different forms of monocotyledons

A technology of monocotyledonous plants and extraction methods, applied in the field of amino acid separation and extraction, can solve the problems of effective separation of amino acids that cannot exist, and achieve far-reaching theoretical and practical application values

Active Publication Date: 2018-06-22
INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The embodiment of the present invention aims at the deficiency that existing extraction methods cannot effectively separate amino acids in various forms, and provides a method for separating and extracting amino acids in different forms of monocotyledonous plants

Method used

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  • Separation and extraction method of amino acid in different forms of monocotyledons
  • Separation and extraction method of amino acid in different forms of monocotyledons
  • Separation and extraction method of amino acid in different forms of monocotyledons

Examples

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Effect test

Embodiment 1

[0026] Step 1, take wheat leaves, cut off 0.5cm at both ends, then cut the wheat leaves into small sections of 3-4cm to obtain wheat leaf sections, weigh 5-10g of the leaf sections, and set aside. According to needs, 0.3-0.8cm can be cut off at both ends of the wheat leaf.

[0027] Step 2: Rinse the incisions of the leaf segments with ultrapure water 2-3 times, then collect them into bundles, and place them in a centrifuge tube. In this embodiment, a double-layer centrifuge tube is used, and the double-layer centrifuge tube includes a centrifuge tube body, which is arranged inside the centrifuge tube body and has a filter sleeve at the bottom. The sleeve is limited on the upper part of the centrifuge tube body, which is used to limit the blade segment bundle and prevent the blade segment bundle from tumbling upward during the vacuuming process. Add 4°C ultrapure water into the centrifuge tube body until it is 1 cm higher than the leaf section, and vacuum the centrifuge tube f...

Embodiment 2

[0048] According to the method of Example 1, the intercellular fluid and intracellular fluid of wheat leaves, the upper part, the middle part of the stem and the lower part of the stem were separated and extracted respectively, and amino acids in different forms were detected. The content of soluble free amino acids in the intracellular fluid is shown in Table 3.

[0049] Table 3 Comparison table of free amino acid content in intracellular fluid of different parts of wheat

[0050]

[0051] According to the results in Table 3, draw a histogram, see figure 1 . It can be seen from the figure that the proportions of free amino acids in the cells of different parts of wheat plants are different, and the levels of each amino acid in the intracellular fluid of leaves are relatively high.

[0052] In summary, the method of the embodiment of the present invention separates, extracts and detects amino acids in different forms in monocotyledonous plants. The method is accurate and ...

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Abstract

The invention discloses a separation and extraction method of amino acid in different forms of monocotyledons, and belongs to the technical field of separation and extraction of amino acid. The methodspecifically comprises the following steps: separating out intercellular washing fluid by carrying out water soaking, vacuumizing and centrifuging on stems or leaves of the monocotyledons; cutting the stems or the leaves from which the intercellular washing fluid is separated out into pieces, pulping in an ice bath and centrifuging the slurry to obtain intracellular fluid and solid residues; respectively detecting the content of soluble free amino acid in the intercellular washing fluid and soluble protein hydrolytic amino acid in the intracellular fluid; detecting the content of insoluble protein hydrolytic amino acid in the solid residues to obtain the content of amino acid in different forms. The method has the advantage that distribution and transportation of the amino acid in plantsare truly reflected, which has great theoretical and practical value for exploring physiology, metabolism and development of plants.

Description

technical field [0001] The invention relates to the technical field of separation and extraction of amino acids, in particular to a method for separation and extraction of amino acids in different forms of monocotyledonous plants. Background technique [0002] Amino acids are the basic units of biologically functional macromolecular proteins. They are synthesized in plant tissues and can also be obtained by proteolysis. They play an important role in the metabolism, growth, maintenance and repair of tissues. Amino acids mainly exist in five forms in plants: ① soluble free amino acids in the interstitial fluid; ② soluble proteolytic amino acids in the interstitial fluid; ③ soluble free amino acids in cells; ④ soluble protein in cells Hydrolyzed amino acids; ⑤ intracellular insoluble proteolytic amino acids. [0003] The functions that amino acids can play through metabolism include: ①synthesis of tissue proteins; ②transformation into acids, hormones, antibodies, creatine and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34
CPCG01N1/286G01N1/34G01N2001/2866
Inventor 唐晓东张丽蒋春志樊翠芹于翠红
Owner INST OF CEREAL & OIL CROPS HEBEI ACAD OF AGRI & FORESTRY SCI
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