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Syphilis detection kit containing CD14 detection reagent and application of reagent in syphilis detection

A detection kit and detection reagent technology, applied in the field of biomedicine, can solve problems such as low specificity

Active Publication Date: 2018-06-22
BEIJING DITAN HOSPITAL CAPITAL MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to reports, there are many markers that can reflect central nervous system damage, such as β-amyloid precursor protein and β-amyloid protein, myelin basic protein, neuron-specific enolase, microtubule-associated protein tau, etc. Drugs also have some changes in patients with neurosyphilis, but there are problems such as low specificity

Method used

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  • Syphilis detection kit containing CD14 detection reagent and application of reagent in syphilis detection
  • Syphilis detection kit containing CD14 detection reagent and application of reagent in syphilis detection
  • Syphilis detection kit containing CD14 detection reagent and application of reagent in syphilis detection

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Experimental program
Comparison scheme
Effect test

Embodiment

[0034] 1. General clinical data of the patient

[0035] Patients in this study were diagnosed with neurosyphilis or latent syphilis according to the WS 273-2007 diagnostic criteria for syphilis.

[0036] 2. Sample acquisition

[0037] According to the conditions of the enrolled patients, with the informed consent of the patients, 5ml of cerebrospinal fluid was collected by lumbar puncture, subpackaged in cryopreservation tubes, coded and marked, stored in a -80°C refrigerator, and finally tested uniformly to avoid batch errors in sample testing.

[0038] 3. Detection method

[0039] The detection methods used in the examples are all known. Among them, the detection of cerebrospinal fluid NF-L adopts (Neurofilament light) ELISA (Enzyme immunoassay for quantitative determination of human Neurofilament light (NF-L) protein in cerebrosphinal fluid) (LBL international GME) was detected according to the instructions. CSF pNF-H was detected using HumanPhosphorylated Neurofilamen...

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Abstract

The invention relates to application of CD14 in syphilis detection, and particularly provides a syphilis detection kit. The kit comprises a detection reagent such as a CD14-resistant antibody for detecting the CD14 level in a biological sample. The invention further provides application of the first detection reagent for detecting CD14 to preparation of the syphilis detection kit. The syphilis detection kit can be used for early-stage diagnosis, parting and staging, therapeutic evaluation and / or prognosis evaluation of syphilis especially neurosyphilis, and has the advantages of being reliable, sensitive, convenient to use and rapid.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of CD14 in the detection of neurosyphilis. Background technique [0002] CD14 is a member of the cell surface glycoprotein family and is a specific surface marker of monocytes, neutrophils and macrophages. It was first discovered by TODD from the surface of human monocytes in 1981. The human gene encoding CD14 has been cloned and sequenced. The gene is located at the long arm end 5q23-q31 of human autosome 5, and contains about 1338 nucleotide residues. From the 76th to the 1200th nucleotide, it encodes a polypeptide chain with 375 amino acid residues. [0003] CD14 can be shed into the blood under the action of endogenous proteolytic enzymes or phosphatases to become sCD14. sCD14 exists in normal human and animal serum, and the normal concentration in human serum is 2 to 5 mg / ml, accounting for 99% of the total CD14 content in blood. In addition, CD14 is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/571G01N33/577
CPCG01N33/571G01N33/577
Inventor 许东梅伦文辉
Owner BEIJING DITAN HOSPITAL CAPITAL MEDICAL UNIV
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