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Extraction method and application of grub protein

An extraction method, grub technology, applied in the direction of peptide preparation method, application, chemical instrument and method, etc., can solve problems such as undiscovered, achieve the effects of reducing deterioration loss, improving protein quality and content, and increasing protein extraction rate

Inactive Publication Date: 2018-06-29
FOSHAN HUIMU CHEM TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Regarding the extraction and utilization of grub protein, no relevant reports have been found so far

Method used

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  • Extraction method and application of grub protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for extracting protein from grubs, comprising the steps of:

[0028] (1) Wash fresh grubs, add distilled water to grind and crush, the mass-to-volume ratio of clean grubs to distilled water is 1:15, and grub slurry is obtained;

[0029] (2) Get the grub slurry of step (1), add the pepsin whose enzyme activity is 55,000 U / g, and the addition amount is 1.5wt% of the grub slurry of step (1), stir evenly, adjust the pH to 2.5, and Carry out enzymatic hydrolysis at 40°C for 45 minutes;

[0030] (3) After the enzymolysis solution in step (2) is subjected to enzyme-inactivating treatment, adjust the pH to 7.5, add trypsin with an enzyme activity of 2500U / g and an addition amount of 1.5wt% of the grub slurry in step (1), and stir Evenly, adjust the pH to 7.5, and carry out enzymatic hydrolysis at 40°C for 45 minutes;

[0031] (4) The enzymolysis solution in step (3) is subjected to an enzyme-inactivating treatment, and then placed in a refrigerator for overnight stor...

Embodiment 2

[0034] A method for extracting protein from grubs, comprising the steps of:

[0035] (1) Wash fresh grubs, add distilled water to grind and crush, the mass volume ratio of clean grubs to distilled water is 1:20, and grub slurry is obtained;

[0036] (2) Get the grub slurry of step (1), add the pepsin whose enzyme activity is 60,000 U / g, and the addition amount is 2wt% of the grub slurry of step (1), stir evenly, adjust the pH to 3.0, at 50 Carry out enzymatic hydrolysis at ℃ for 30 minutes;

[0037] (3) After the enzymolysis solution in step (2) is subjected to enzyme-inactivating treatment, adjust the pH to 7.0, add trypsin with an enzyme activity of 2000U / g and an addition amount of 1wt% of the grub slurry in step (1), and stir evenly , adjust the pH to 7.0, and carry out enzymatic hydrolysis at 30°C for 60 minutes;

[0038] (4) The enzymolysis solution in step (3) is subjected to an enzyme-inactivating treatment, and then placed in a refrigerator for overnight storage, th...

Embodiment 3

[0041] A method for extracting protein from grubs, comprising the steps of:

[0042] (1) Wash fresh grubs, add distilled water to grind and crush, the mass volume ratio of clean grubs to distilled water is 1:10, and grub slurry is obtained;

[0043] (2) Get the grub slurry of step (1), add enzyme activity and be 50,000 U / g, add the pepsin that is 1wt% of step (1) grub slurry, stir, adjust pH to 2.0, at 30 Carry out enzymatic hydrolysis at ℃ for 60 minutes;

[0044] (3) After the enzymolysis solution in step (2) is subjected to enzyme-inactivating treatment, adjust the pH to 8.0, add trypsin with an enzyme activity of 3000U / g and an addition amount of 2wt% of the grub slurry in step (1), and stir evenly , adjust the pH to 8.0, and carry out enzymatic hydrolysis at 50°C for 30 minutes;

[0045] (4) The enzymolysis solution in step (3) is subjected to an enzyme-inactivating treatment, and then placed in a refrigerator for overnight storage, the enzymolysis solution is filtered,...

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Abstract

The invention discloses an extraction method and an application of a grub protein and provides a compound protein beverage. The extraction method of the grub protein mainly comprises the following steps of cleaning, grinding, enzymolysis treatment, deactivation and filtration. The fresh protein is extracted from live grubs by a two-enzyme method, and the compound protein that is higher in qualityand content can be obtained and used for supplementing various proteins required by an animal.

Description

technical field [0001] The invention relates to a method for extracting protein, in particular to a method for extracting protein from grubs. Background technique: [0002] Grubs, also known as cockroaches, soil silkworms, old mother worms, white time worms, etc., are hypertrophied, and they are commonly called scarabs or scarabs when they become adults, and more than half of their composition is fat and protein. As a protein source with rich ingredients, most of them are simply crushed and made into feed or other uses, and the effective utilization rate of protein is low. [0003] Insect protein is rich in components, which can comprehensively supplement the nutrition needed by animals, effectively promote the recovery of weak constitution and malnutrition, and help to further strengthen the immune defense function of the body. And adding it into the feed has just obtained the compound protein liquid feed. [0004] The main function of compound protein liquid feed is to s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A23K20/147
CPCA23K20/147C07K1/34C12P21/06
Inventor 不公告发明人
Owner FOSHAN HUIMU CHEM TECH CO LTD