Method for preparing slowly digestible starch by utilizing double enzymes

A slow-digesting starch and starch technology, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, metabolic diseases, etc., can solve problems that have not been reported in the literature, and achieve the goal of reducing the digestion rate, increasing the content, and promoting synergy Effect

Active Publication Date: 2018-07-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The catalytic mechanism of these two enzymes is beneficial to the formation of slow-digesting starch, and the method of synergistic modification of starch by the two enzymes has not been reported in the literature.

Method used

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  • Method for preparing slowly digestible starch by utilizing double enzymes
  • Method for preparing slowly digestible starch by utilizing double enzymes
  • Method for preparing slowly digestible starch by utilizing double enzymes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0025] Example 3: Effect of double enzyme synergistic modification on slow digestible starch content in starch

[0026] Dissolve cornstarch in water to obtain 5% starch milk, gelatinize in boiling water, add 3U / g starch cyclodextrin glucosyltransferase and 25U / g starch starch branching enzyme at the same time, treat at 45°C for 16h, boil water bath The reaction was terminated, and the modified starch was obtained by freeze-drying. Refer to Englyst in vitro simulated digestion method to determine the digestibility of modified starch, as shown in Table 1. The results showed that compared with Example 1, the content of slow digestible starch increased by 55.1%; compared with Example 2, the content of slow digestible starch increased by 2.1%, and the content of resistant starch increased by 13.8%.

Embodiment 4

[0027] Example 4: Effects of double-enzyme addition methods on slow-digestible starch content in starch

[0028] Dissolve corn starch in water to obtain 5% starch milk. After gelatinization in boiling water, add 3 U / g starch cyclodextrin glucosyltransferase at a constant temperature of 45°C for 8 hours, then add 25 U / g starch starch branching enzyme to continue the reaction After 8 hours, the reaction was terminated in a boiling water bath, and the modified starch was obtained by freeze-drying. Refer to Englyst in vitro simulated digestion method to determine the digestibility of modified starch, as shown in Table 1. The results showed that compared with the control group, the fast digestible starch content decreased by 47.2%, the slow digestible starch content increased by 119%, and the resistant starch content increased by 210%. Compared with Example 3, the fast digestible starch content was reduced by 13.2%, and the digestion rate of the modified sample was further reduced...

Embodiment 5

[0029] Embodiment 5: The effect of double-enzyme addition method on the content of slowly digestible starch in starch

[0030] Dissolve cornstarch in water to obtain 5% starch milk. After gelatinization in boiling water, add 25U / g starch starch branching enzyme at a constant temperature of 45°C for 8 hours, then add 3U / g starch cyclodextrin glucosyltransferase to continue the reaction After 8 hours, the reaction was terminated in a boiling water bath, and the modified starch was obtained by freeze-drying. Refer to Englyst in vitro simulated digestion method to determine the digestibility of modified starch, as shown in Table 1. The results showed that compared with Example 3, the content of fast digestible starch was reduced by 2.56%, and the effect was not significant.

[0031] A comprehensive comparison shows that the addition of enzymes will have different degrees of influence on the preparation of slow digestible starch. First cyclodextrin glucosyltransferase treatment 8...

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Abstract

The invention discloses a method for preparing slowly digestible starch by utilizing double enzymes and belongs to the field of biologically modified starch. According to the method disclosed by the invention, the starch is cooperatively treated by utilizing cyclodextrin glycosyltransferase and a starch branching enzyme; the cyclodextrin glycosyltransferase is catalyzed to generate cyclodextrin and a lot of short-chain segments are generated; a fine and long type starch molecule is converted into a short and fat structure which is a tighter branch structure under the action of the starch branching enzyme, so that the slow digestion of the starch is more remarkable. By changing an adding manner of the two enzymes, an enzyme adding amount and reaction time, a cooperative effect between the two enzymes is promoted, the content of the cyclodextrin and the branching degree of amylopectin are improved; the content of the slowly digestible starch and resisting starch is further increased andthe digestion speed of the starch is reduced; a novel concept is provided for preparing the slowly digestible starch through biological modification.

Description

technical field [0001] The invention relates to a method for preparing slow-digesting starch by using double enzymes, which belongs to the field of bio-modified starch. Background technique [0002] Starch is the main component of green plant fruits, seeds, tubers and roots, and is the most abundant storage polysaccharide on the earth. As the main source of energy for humans and most animals, it is often used in food, medicine, chemical and other industrial fields. In the diet of residents, the "quality" and "quantity" of starch directly affect the regulation of blood sugar. With the improvement of people's living standards, the incidence of chronic diseases such as diabetes has increased year by year in recent years. It is imperative to research and prepare slow-digesting starch and resistant starch that can release energy continuously, avoid violent fluctuations in blood sugar, and have adjuvant therapeutic effects on non-insulin-dependent diabetes and cardiovascular dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/18C12P19/04A61K31/724A61P3/10A61P9/00
CPCA61K31/724C12P19/04C12P19/18
Inventor 李兆丰顾正彪李阳李才明任俊彦程力洪雁
Owner JIANGNAN UNIV
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