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Application of ano5 in the preparation of drugs for treating pancreatic cancer

A technology of pancreatic cancer cells and drugs, applied in drug combinations, pharmaceutical formulations, anti-tumor drugs, etc., can solve the problems of unreported effects on tumor cells

Active Publication Date: 2021-06-01
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] ANO5 is widely expressed in the epithelial cells of the digestive tract, but its expression in normal pancreatic tissue and pancreatic cancer cells and its effect on tumor cells have not been reported

Method used

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  • Application of ano5 in the preparation of drugs for treating pancreatic cancer
  • Application of ano5 in the preparation of drugs for treating pancreatic cancer
  • Application of ano5 in the preparation of drugs for treating pancreatic cancer

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Experimental program
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Embodiment 1

[0025] 1. Materials and methods

[0026] 1.1 Materials: Human pancreatic cancer tissue was purchased from Xi'an Alina Biotechnology Co., Ltd.; human pancreatic cancer cells HPAC cells, PANC-1 cells, BxPC-3 cells, HEK293 cells, human normal pancreatic ductal epithelial cells (HPDE6-C7 cells) Purchased from Wuhan University Cell Bank, China Cell Resource Bank.

[0027] 1.2 Main reagents: ANO5 antibody, American Biorbyt Company, immunohistochemical staining kit (SPN-9001)

[0028] 1.3 Experimental method

[0029] 1.3.1 Immunohistochemistry

[0030] Tissue sections were deparaffinized with xylene, hydrated with graded ethanol, and 3% HO 2 o 2 Block at room temperature for 10 minutes to eliminate endogenous peroxidase, citrate antigen repair solution for microwave heat repair, normal goat serum for 1 hour at room temperature, drop ANO5 antibody (1:100) and incubate overnight at 4°C, rewarm and drop Add reagent B in the immunohistochemical staining kit, add reagent C in the imm...

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Abstract

The invention relates to the application of ANO5 (Anoctamin5) in the preparation of pancreatic cancer diagnostic markers and drugs for treating pancreatic cancer. The present invention utilizes CCK‑8 experiment and scratch experiment to detect the effect of transfection of ANO5siRNA on the proliferation and migration of pancreatic cancer cells. ANO5 can inhibit the proliferation and migration ability of pancreatic cancer cells, so ANO5 can become a new target for pancreatic cancer treatment, and ANO5 siRNA can be used to prepare drugs for treating pancreatic cancer.

Description

[0001] (1) Technical field [0002] The invention relates to the application of ANO5 (Anoctamin5) in the preparation of pancreatic cancer diagnostic markers and drugs for treating pancreatic cancer. [0003] (2) Background technology [0004] Pancreatic cancer is a malignant tumor with high morbidity and mortality, which seriously threatens human health. The mechanism of its occurrence and development has always been a hot spot for researchers. In recent years, the relationship between ion channels and tumors has received more and more attention. The TMEM16 transmembrane protein family (TMEM16A~K), also known as the anoctamin family (anoctamin1-10), is a calcium ion-activated chloride channel protein family. Studies have shown that , and its multiple family members play an important role in the occurrence of tumors and the phenotype maintenance of tumor cells. [0005] ANO5 is widely expressed in the epithelial cells of various parts of the digestive tract, but its expression ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886A61K31/7105A61P35/00
CPCA61K31/7105C12Q1/6886C12Q2600/178
Inventor 宋海岩张毅敏周立连辉王志勇
Owner XINXIANG MEDICAL UNIV