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Modified ccp antigen and use thereof, anti-ccp antibody detection kit and manufacturing method thereof

A technology of antibody detection and kit, applied in the field of anti-CCP antibody detection kit and its manufacture

Active Publication Date: 2020-07-14
SHENZHEN NEW INDS BIOMEDICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The existing technology still has higher requirements for the antigenic activity of the derivatives of the CCP antigen-linked protein carrier, and there are higher requirements for the precision and accuracy of anti-CCP antibody detection

Method used

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  • Modified ccp antigen and use thereof, anti-ccp antibody detection kit and manufacturing method thereof
  • Modified ccp antigen and use thereof, anti-ccp antibody detection kit and manufacturing method thereof
  • Modified ccp antigen and use thereof, anti-ccp antibody detection kit and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0110] The polypeptide sequence of the novel CCP antigen used in this example is: HQCHQE(R)ST-cit-GRSRGRCGRSGS, disulfide bond: C3=C16, wherein the R group is methoxy CH 3 O-.

[0111] The kit includes the following components:

[0112] 1) Component A: new CCP antigen derivative solution coated with magnetic balls, the new CCP antigen derivatives are formed by coupling the new CCP antigen and bovine IgG protein carrier, in which: the working concentration of the magnetic balls: 1mg / mL, new The working concentration of CCP antigen is 50ng / mL, and the concentration of bovine IgG protein carrier is 10ng / mL;

[0113] 2) Component B: mouse anti-human IgG secondary antibody-labeled ABEI solution, wherein the working concentration of ABEI is 30 ng / mL, and the working concentration of mouse anti-human IgG secondary antibody is 300 ng / mL.

[0114] 3) Calibrator solution: a low-concentration calibrator solution with a concentration of 3.91 U / mL and a high-concentration calibrator solu...

Embodiment 2

[0129] The R group of the novel CCP antigen used in this example is pentyloxy CH 3 (CH 2 ) 4 O-, all the other are identical with embodiment 1.

Embodiment 3

[0131] The R group of the novel CCP antigen used in this example is acetamide (CH3CHO-NH-), and the rest are the same as in Example 1.

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PUM

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Abstract

The invention belongs to the technical field of in vitro diagnoses and particularly relates to a modified CCP antigen. The modified CCP antigen has the sequence shown as a formula I, and the formula Iis shown in the description, wherein a carboxyl hydroxyl group of glutamic acid E in the formula I is substituted by an R group selected from the group consisting of C1-10 alkyloxy, C1-10 alkyl-CO-NH-yl and C1-10 alkyl-CO-NH-NH-yl; the C1-10 alkyl is substituted by any one or more substituent groups selected from the group consisting of C1-4 alkyl, C1-4 alkyloxy, hydroxy, amino, nitro and phenyl.The invention also relates to the use of the antigen for detecting an anti-CCP antibody, an anti-CCP antibody detection kit comprising the antigen and a manufacturing method of the kit. The modifiedCCP antigen and use thereof and the anti-CCP antibody detection kit and the manufacturing method thereof have the advantages that the antigenic activity of derivatives after the modified CCP antigen is connected to protein carriers is higher, and the precision and accuracy of anti-CCP antibody detection are higher.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis, and specifically relates to a modified CCP antigen, which is used for detecting anti-CCP antibodies, including an anti-CCP antibody detection kit for the antigen and a manufacturing method thereof. Background technique [0002] Rheumatoid Arthritis (RA) is a multisystem, inflammatory, autoimmune, and teratogenic disease mainly involving peripheral joints. The incidence rate of the disease is 1% in European and American countries, and 0.32% to 0.36% in my country. Irreversible bone and joint destruction can occur within 2 years of the onset of the disease, which is one of the main reasons for the loss of labor force in the population. [0003] Guo Dawen and others found that anti-cyclic citrullinated peptide antibodies have predictive value for the onset of rheumatoid arthritis (Guo Dawen, Wang Ye, Gao Honghua, et al. The predictive value of anti-cyclic citrullinated peptide antibodies...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/50G01N33/533
CPCG01N33/533G01N33/54326
Inventor 饶微魏亚庆袁锦云刘蕾李婷华许然
Owner SHENZHEN NEW INDS BIOMEDICAL ENG