Method for solid fermentation of wheat bran with cordyceps militaris

A technology of solid-state fermentation and Cordyceps militaris, which is applied in animal feed, animal feed, applications, etc., can solve the problems of artificial Cordyceps militaris being expensive, frequent degradation of strains, shortening fermentation time, etc. Oxidative activity and low operating environment requirements

Inactive Publication Date: 2018-07-17
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fruiting body has a long production cycle and is restricted by many factors such as climatic conditions, especially the frequent degradation of the strains during the production process, which makes the price of artificial Cordyceps militaris very expensive
Some domestic scholars try to concentrate the research direction of Cordyceps militaris on solid-state fermentation, so as not to allow its growth body to obtain the fungus of Cordyceps militaris, which not only shortens the fermentation time and reduces the cost, but also its rich mycelium ensures the unique value of Cordyceps militaris However, there are few foreign reports on the solid-state fermentation of Cordyceps militaris using agricultural and sideline products as fermentation substrates.

Method used

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  • Method for solid fermentation of wheat bran with cordyceps militaris

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Embodiment 1

[0033] Embodiment 1 A method of solid-state fermentation of wheat bran by Cordyceps militaris

[0034] A method for solid-state fermentation of wheat bran by Cordyceps militaris, comprising the following methods:

[0035] 1. Prepare slant medium and inoculate strains. Weigh 200g of peeled potatoes and add them to 1L of water, heat to boiling and maintain for 15min, filter and take the filtrate to replenish water. Add 20g of glucose, 1g of magnesium sulfate, 3g of potassium dihydrogen phosphate, and 5 tablets of VB to the filtrate 1 , agar 20g, sterilized at 121°C for 20min. Cultivate at 37° C. for 24 hours, and put the Cordyceps militaris strains on the culture medium without the growth of miscellaneous bacteria.

[0036] 2. Preparation of liquid strains Take two rings of slant strains and inoculate them in a 500mL Erlenmeyer flask (filling volume: 150mL), culture in a shaker in the dark, set the temperature at 25°C, and rotate at 100r / min, until uniform rice-grain-sized Th...

Embodiment 2

[0038] Embodiment 2 A method of solid-state fermentation of wheat bran by Cordyceps militaris

[0039] In embodiment 2, step (1) and (2) are with specific embodiment 1, and difference is in step (3), specifically as follows:

[0040] Adjust the moisture content of the wheat bran to 53%, sterilize at 121°C for 60 minutes, cool to room temperature, aseptically insert the cultured Cordyceps militaris liquid strain, the inoculation amount is 11%, put it in a 33cm bacteria bag, and use it at 5cm at both ends The cap (the diameter of the sterile gas-permeable membrane is 5cm) is tied, and the filling amount is 300g / bag. Set the culture temperature to 25°C, culture for 11 days to end the fermentation, measure the content of cordycepin and various nutrients in the fungus, and measure the antioxidant capacity.

Embodiment 3

[0041] Embodiment 3 A method of solid-state fermentation of wheat bran by Cordyceps militaris

[0042] In embodiment 2, step (1) and (2) are with specific embodiment 1, and difference is in step 3, specifically as follows:

[0043] Adjust the moisture content of wheat bran to 45%, sterilize at 121°C for 60 minutes, cool to room temperature, aseptically insert the cultured Cordyceps militaris liquid strain, the inoculum amount is 16%, put it in a 33cm bacteria bag, and use it at 5cm at both ends The cap (the diameter of the sterile gas-permeable membrane is 5cm) is tied, and the filling amount is 300g / bag. The culture temperature was set at 22°C, the fermentation was completed after 20 days of culture, and the content of cordycepin and various nutrients in the fungus was measured, and the antioxidant capacity was measured.

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Abstract

The invention relates to a solid fermentation method, in particular to a method for solid fermentation of wheat bran. In order to increase the edible and medical value of wheat bran, the invention provides a method for solid fermentation of wheat bran with cordyceps militaris. The method comprises the following steps of (1) preparing an agarslantculture-medium and performing inoculation with stains; (2) preparing liquid stains; and (3) performing wheat bran solid fermentation. According to the method disclosed by the invention, the content of soluble dietary fibers in the wheat bran is increased to 10.96% from 3.54%, the protein content is increased to 20.51% from 16.40%, the phyic acid content is reduced to 8mg/g from 26.3mg/g, the total phenol content achieves 2.95mg GAE/g from 1.81mg GAE, the cordycepic acid content achieves 260mg, and the cordyceps polysaccharide content achieves 17.57mg from 9.65mg. In addition, the oxidation resistance is also improved. According to the method for solid fermentation of wheat bran with cordyceps militaris, the drug effects of the cordyceps militaris and the nutrients of the wheat bran are organically combined, so that the edible and medical value of the wheat bran is notably increased, and medical components of the cordyceps militaris can be safe and convenient to use.

Description

technical field [0001] The invention relates to a method for solid-state fermentation, in particular to a method for solid-state fermentation of wheat bran by Cordyceps militaris. Background technique [0002] As a by-product of wheat processing, wheat bran is rich in starch, cellulose, protein, phenolic substances, various minerals and various trace elements. my country is a large wheat producing country, so wheat bran has a wide range of sources, sufficient reserves, and low price. However, due to the rough taste of wheat bran, there are few nutrients that can be directly used. At present, most of the wheat bran is used for feed processing, wine making, and vinegar making. . In recent years, with the deepening of research, the bioutilization value of various wheat bran has gradually been recognized, and the extraction of various nutrients from wheat bran, or the use of wheat bran for fermentation has gradually become a research hotspot. [0003] At present, the commonly u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/37A23K10/12
CPCA23K10/12A23K10/37Y02P60/87
Inventor 张锦丽侯汉学张宪省董海洲
Owner SHANDONG AGRICULTURAL UNIVERSITY
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