10-hcpt-loaded phase-change lipid nanoparticles modified by HA-mediated cpps and preparation method thereof

Abstract

The invention discloses HA mediated CPPs modified 10-HCPT carried phase change lipid nanoparticles. The nanoparticles include a phospholipid shell membrane, the phospholipid shell membrane carries anti-tumor drugs 10-HCPT, DC-cholesterol is modified on the phospholipid shell membrane, cysteine cell-penetrating peptides are connected to the two sides, and hyaluronic acid is adsorbed to the outer layer of the phospholipid shell membrane. A preparation method of the HA mediated CPPs modified 10-HCPT carried phase change lipid nanoparticles has the advantages that the nanoparticles can achieve specific active targeting and enter deep liver cancer tissue, liquid-gas phase transformation can be performed under in-vitro LIFU radiation, and real-time development and positioned release of anti-tumor drugs can be achieved at the same time.

Description

technical field [0001] The invention relates to the field of ultrasound imaging, in particular to a preparation method and application of HA-mediated CPPs-loaded phase-change lipid nanoparticles loaded with 10-HCPT. Background technique [0002] Hepatocellular carcinoma (HCC) is a malignant tumor with the fifth highest incidence rate and the third highest mortality rate, which greatly endangers the physical and mental health of patients. At present, the best treatment for HCC is still radical surgical resection, but most HCC patients are diagnosed at an advanced stage or have distant metastasis, so the treatment is difficult and the prognosis is poor. Because primary liver cancer is less sensitive to existing systemic chemotherapy drugs, its chemotherapy effect is not good. Therefore, there is an urgent need to explore new targets, new measures and develop new drugs for the treatment of primary liver cancer. [0003] As an ultrasound contrast agent, ultrasonic microbubbles...

AI Technical Summary

Problems solved by technology

[0003] As an ultrasound contrast agent, ultrasonic microbubbles have been widely used in the diagnosis of clinical diseases, especially in the diagnosis and differential diagnosis of liver diseases. In addition, as a tool for carrying anti-tumor drugs or genes, they are also used in the treatment of diseases. Widely used in experimental research, but ultrasonic microbubbles cannot pass through the vascular endothelial space (380-780nm) inside the tumor tissue due to their large particle size, making it difficult to achieve imaging and treatment of extravascular tumor tissue

In order to solve this problem, lipid nanoparticles can penetrate the vascular endothelial space of tumor tissue to reach the tumor tissue with a nano-scale particle size, and can also be used as an anti-tumor drug carrier to transport chemotherapy drugs to the tumor site for anti-cancer treatment. Nanoparticles enhance backscatter visualization in aggregated form, which is less effective than ultrasound microbubbles

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